Anthony P. Pugsley

Anthony P. Pugsley's AcademicInfluence.com Rankings

Anthony P. Pugsley

Biology

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Microbiology

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#496

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Biology

Anthony P. Pugsley's Degrees

PhD Microbiology Stanford University
Bachelors Biology University of California, Berkeley

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Anthony P. Pugsley's Published Works

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Total number of citations to an author for the works they published in a given year. This highlights publication of the most important work(s) by the author

Published Works

The complete general secretory pathway in gram-negative bacteria. (1993) (1851)
Type II protein secretion and its relationship to bacterial type IV pili and archaeal flagella. (2003) (377)
Insertion of an outer membrane protein in Escherichia coli requires a chaperone‐like protein. (1996) (259)
Pilus formation and protein secretion by the same machinery in Escherichia coli (2000) (242)
Export and secretion of proteins by bacteria (1985) (238)
Secretin PulD: association with pilot PulS, structure, and ion-conducting channel formation. (1999) (215)
Cloning and expression in Escherichia coli of the Klebsiella pneumoniae genes for production, surface localization and secretion of the lipoprotein pullulanase. (1987) (211)
Testing the ‘+2 rule’ for lipoprotein sorting in the Escherichia coli cell envelope with a new genetic selection (1999) (207)
The ins and outs of colicins. Part I: Production, and translocation across membranes. (1984) (177)
Secretion by numbers: protein traffic in prokaryotes (2006) (159)
Structural Insights into the Secretin PulD and Its Trypsin-resistant Core* (2005) (149)
Colicin E2 release: lysis, leakage or secretion? Possible role of a phospholipase. (1984) (149)
Genetics of extracellular protein secretion by gram-negative bacteria. (1990) (147)
Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion (2000) (146)
Multiple Interactions between Pullulanase Secreton Components Involved in Stabilization and Cytoplasmic Membrane Association of PulE (2000) (142)
Domain structure of secretin PulD revealed by limited proteolysis and electron microscopy (2000) (142)
Extracellular pullulanase of Klebsiella pneumoniae is a lipoprotein (1986) (140)
Protein secretion by gram-negative bacteria. Characterization of two membrane proteins required for pullulanase secretion by Escherichia coli K-12. (1989) (139)
Major heat-modifiable outer membrane protein in gram-negative bacteria: comparison with the ompA protein of Escherichia coli (1980) (138)
Methods for studying colicins and their plasmids (1987) (135)
Type IV-Like Pili Formed by the Type II Secreton: Specificity, Composition, Bundling, Polar Localization, and Surface Presentation of Peptides (2003) (131)
Bacterial outer membrane secretin PulD assembles and inserts into the inner membrane in the absence of its pilotin (2006) (129)
Identification of three genes controlling production of new outer membrane pore proteins in Escherichia coli K-12 (1978) (128)
Molecular characterization of PulE, a protein required for pullulanase secretion (1994) (126)
Molecular genetics in mycobacteria. (1987) (125)
Outer Membrane Proteins of Escherichia coli VII. Evidence That Bacteriophage-Directed Protein 2 Functions as a Pore (1978) (125)
The secretin‐specific, chaperone‐like protein of the general secretory pathway: separation of proteolytic protection and piloting functions (1996) (119)
Recent progress and future directions in studies of the main terminal branch of the general secretory pathway in Gram-negative bacteria--a review. (1997) (117)
Identification, mapping, cloning and characterization of a gene (sbmA) required for microcin B17 action on Escherichia coli K12. (1986) (117)
Pullulanase secretion in Escherichia coli K‐12 requires a cytoplasmic protein and a putative polytopic cytoplasmic membrane protein (1992) (115)
Structure and assembly of the pseudopilin PulG (2004) (110)
Escherichia coli K12 strains for use in the identification and characterization of colicins. (1985) (108)
Transcription and expression analysis, using lacZ and phoA gene fusions, of Mycobacterium fortuitumβ‐lactamase genes cloned from a natural isolate and a high‐level β‐lactamase producer (1994) (107)
The ins and outs of colicins. Part II. Lethal action, immunity and ecological implications. (1984) (104)
High-molecular-weight protein 2 of Yersinia enterocolitica is homologous to AngR of Vibrio anguillarum and belongs to a family of proteins involved in nonribosomal peptide synthesis (1993) (103)
Depletion of Apolipoprotein N-Acyltransferase Causes Mislocalization of Outer Membrane Lipoproteins in Escherichia coli* (2005) (103)
The C‐terminal domain of the secretin PulD contains the binding site for its cognate chaperone, PulS, and confers PulS dependence on pIVf1 function (1997) (103)
Klebsiella pneumoniae pulS gene encodes an outer membrane lipoprotein required for pullulanase secretion (1989) (100)
Translocation of a folded protein across the outer membrane in Escherichia coli. (1992) (100)
Processing and methylation of PulG, a pilin‐like component of the general secretory pathway of Klebsiella oxytoca (1993) (97)
Genetic Dissection of the Outer Membrane Secretin PulD: Are There Distinct Domains for Multimerization and Secretion Specificity? (1999) (96)
Characterization of group B colicin-resistant mutants of Escherichia coli K-12: colicin resistance and the role of enterochelin (1976) (95)
Minor pseudopilin self‐assembly primes type II secretion pseudopilus elongation (2012) (94)
The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins (1996) (93)
Characterization and expression of the structural gene for pullulanase, a maltose-inducible secreted protein of Klebsiella pneumoniae (1985) (93)
Iron uptake in colicin B-resistant mutants of Escherichia coli K-12 (1976) (87)
Identification of two regions of Klebsiella oxytoca pullulanase that together are capable of promoting β‐lactamase secretion by the general secretory pathway (1996) (86)
The BtuB Group Col Plasmids and Homology Between the Colicins They Encode (1982) (86)
Microcin E492, a low-molecular-weight peptide antibiotic which causes depolarization of the Escherichia coli cytoplasmic membrane (1985) (79)
Export and secretion of the lipoprotein Pullulanase by Klebsiella pneumoniae (1987) (79)
Five genes at the 3′ end of the Klebsiella pneumoniae pulC operon are required for pullulanase secretion (1990) (79)
Factors affecting the electrophoretic mobility of the major outer membrane proteins of Escherichia coli in polyacrylamide gels. (1979) (78)
An enzyme with type IV prepilin peptidase activity is required to process components of the general extracellular protein secretion pathway of Klebsiella oxytoca (1992) (75)
Remodeling a DNA-binding protein as a specific in vivo inhibitor of bacterial secretin PulD (2007) (75)
PpdD Type IV Pilin of Escherichia coliK-12 Can Be Assembled into Pili in Pseudomonas aeruginosa (2000) (73)
Two distinct steps in pullulanase secretion by Escherichia coli K12 (1991) (72)
Novel Inner Membrane Retention Signals in Pseudomonas aeruginosa Lipoproteins (2008) (72)
The general protein‐export pathway is directly required for extracellular pullulanase secretion in Escherichia coli k12 (1991) (71)
Energy requirement for pullulanase secretion by the main terminal branch of the general secretory pathway (1997) (71)
Signal Recognition Particle-Dependent Inner Membrane Targeting of the PulG Pseudopilin Component of a Type II Secretion System (2006) (71)
Nucleotide sequencing of the structural gene for colicin N reveals homology between the catalytic, C‐terminal domains of colicins A and N (1987) (70)
In vitro multimerization and membrane insertion of bacterial outer membrane secretin PulD. (2008) (70)
The normally periplasmic enzyme β‐lactamase is specifically and efficiently translocated through the Escherichia coli outer membrane when it is fused to the cell‐surface enzyme pullulanase (1990) (69)
Direct Visualization of Red Fluorescent Lipoproteins Indicates Conservation of the Membrane Sorting Rules in the Family Enterobacteriaceae (2006) (69)
Type III Protein Translocase (2003) (68)
Secretins take shape (2006) (66)
Molecular characterization of pulA and its product, pullulanase, a secreted enzyme of Klebsielia pneumoniae UNF5023 (1990) (63)
Molecular characterization of malQ, the structural gene for the Escherichia coli enzyme amylomaltase (1988) (62)
Type III protein translocase: HrcN is a peripheral ATPase that is activated by oligomerization. (2003) (61)
The general secretory pathway of Klebsiella oxytoca: no evidence for relocalization or assembly of pilin‐like PulG protein into a multiprotein complex (1993) (60)
YaeT‐independent multimerization and outer membrane association of secretin PulD (2007) (59)
Sorting of an integral outer membrane protein via the lipoprotein‐specific Lol pathway and a dedicated lipoprotein pilotin (2011) (59)
Defective growth functions in mutants of Escherichia coli K12 lacking a major outer membrane protein. (1977) (59)
Multimers of the precursor of a type IV pilin‐like component of the general secretory pathway are unrelated to pili (1996) (59)
Towards the Identification of Type II Secretion Signals in a Nonacylated Variant of Pullulanase from Klebsiella oxytoca (2005) (57)
Uptake of ferrienterochelin by Escherichia coli: energy dependent stage of uptake (1977) (53)
Membrane Association and Multimerization of Secreton Component PulC (1999) (53)
The role of colicin receptors in the uptake of ferrienterochelin by Escherichia coli K-12. (1977) (51)
Type II Secretion System Secretin PulD Localizes in Clusters in the Escherichia coli Outer Membrane (2008) (50)
Expression of a gene in a 400-base-pair fragment of colicin plasmid ColE2-P9 is sufficient to cause host cell lysis (1983) (50)
Secretion of the cell surface lipoprotein pullulanase in Escherichia coli. Cooperation or competition between the specific secretion pathway and the lipoprotein sorting pathway. (1991) (49)
In vivo effects of local anesthetics on the production of major outer membrane proteins by Escherichia coli. (1980) (47)
Analysis of the subcellular location of pullulanase produced by Escherichia coli carrying the pulA gene from Klebsiella pneumoniae strain UNF5023 (1990) (46)
Neisseria gonorrhoeae prepilin export studied in Escherichia coli (1991) (46)
PuIO, a component of the pullulanase secretion pathway of Klebsiella oxytoca, correctly and efficiently processes gonococcal type IV prepilin in Escherichia coli (1992) (45)
Getting out: protein traffic in prokaryotes (2004) (45)
Genetic analysis of ColN plasmid determinants for colicin production, release, and immunity (1984) (44)
Molecular characterisation of the colicin E2 operon and identification of its products (2004) (44)
Coupled cell-free synthesis and lipid vesicle insertion of a functional oligomeric channel MscL MscL does not need the insertase YidC for insertion in vitro. (2011) (44)
Artificial binding proteins (Affitins) as probes for conformational changes in secretin PulD. (2008) (44)
Outer Membrane Targeting of Secretin PulD Protein Relies on Disordered Domain Recognition by a Dedicated Chaperone*♦ (2011) (44)
Release of colicin E2 from Escherichia coli (1981) (43)
Outer Membrane Targeting of Pseudomonas aeruginosa Proteins Shows Variable Dependence on the Components of Bam and Lol Machineries (2011) (43)
A gene for a new lipoprotein in the dapA-purC interval of the Escherichia coli chromosome (1991) (42)
The ChiA (YheB) protein of Escherichia coli K‐12 is an endochitinase whose gene is negatively controlled by the nucleoid‐structuring protein H‐NS (2000) (41)
Increased production of the outer membrane receptors for colicins B, D and M by Escherichia coli under iron starvation. (1976) (40)
Disulfide Bond Formation in Secreton Component PulK Provides a Possible Explanation for the Role of DsbA in Pullulanase Secretion (2001) (40)
Stable periplasmic secretion intermediate in the general secretory pathway of Escherichia coli. (1993) (40)
Measure for measure in the control of type III secretion hook and needle length (2005) (39)
Protein secretion in Escherichia coli K-12: dead or alive? (1998) (39)
Highly efficient uptake of a rifamycin derivative via the FhuA-TonB-dependent uptake route in Escherichia coli. (1987) (39)
The role of the lipoprotein sorting signal (aspartate +2) in pullulanase secretion (1993) (38)
Chemical heterogeneity of major outer membrane pore proteins of Escherichia coli (1979) (38)
Pilotin–secretin recognition in the type II secretion system of Klebsiella oxytoca (2011) (38)
The conserved tetracysteine motif in the general secretory pathway component PulE is required for efficient pullulanase secretion. (1997) (38)
An unmodified form of the ColE2 lysis protein, an envelope lipoprotein, retains reduced ability to promote colicin E2 release and lysis of producing cells. (1987) (35)
Microcin-E492-insensitive mutants of Escherichia coli K12. (1986) (35)
A second prepilin peptidase gene in Escherichia coli K‐12 (1998) (34)
ActA is a dimer. (1997) (34)
Structural similarity of secretins from type II and type III secretion systems. (2014) (33)
Extracellular secretion of pullulanase is unaffected by minor sequence changes but is usually prevented by adding reporter proteins to its N- or C-terminal end (1995) (32)
Type IV prepilin peptidase gene of Neisseria gonorrhoeae MS11: presence of a related gene in other piliated and nonpiliated Neisseria strains (1994) (30)
The requirement for DsbA in pullulanase secretion is independent of disulphide bond formation in the enzyme (1998) (30)
Changes in host cell phospholipid composition of φX174 gene E product (1985) (29)
Correlation Between the Expression of an Escherichia coli Cell Surface Protein and the Ability of the Protein to Bind to Lipopolysaccharide (1980) (28)
Five additional genes in thepulC-O operon of the gram-negative bacteriumKlebslella oxytoca UNF5023 which are required for pullulanase secretion (1990) (28)
A gene fusion approach to the study of pullulanase export and secretion in Eschenchia coli (1987) (27)
Multimerization-defective variants of dodecameric secretin PulD. (2011) (27)
15 – Protein Secretion across the Outer Membrane of Gram-Negative Bacteria (1988) (27)
Structural Basis of Pullulanase Membrane Binding and Secretion Revealed by X-Ray Crystallography, Molecular Dynamics and Biochemical Analysis. (2016) (27)
Shiga toxin. (1986) (26)
Green Fluorescent Chimeras Indicate Nonpolar Localization of Pullulanase Secreton Components PulL and PulM (2006) (25)
An intramolecular disulphide bond reduces the efficacy of a lipoprotein plasma membrane sorting signal (2003) (24)
Comparison of Colicins B-K260 and D-CA23: Purification and Characterization of the Colicins and Examination of Colicin Immunity in the Producing Strains (1977) (24)
The complete general secretory pathway in gram-negative bacteria (1993) (20)
Mode of action of diptericin A, a bactericidal peptide induced in the hemolymph of Phormia terranovae larvae (1989) (20)
A genetic approach to the study of mitomycin-induced lysis of Escherichia coli K-12 strains which produce colicin E2 (2004) (20)
Obligatory coupling of colicin release and lysis in mitomycin-treated Col+ Escherichia coli. (1983) (20)
Translocation of proteins with signal sequences across membranes. (1990) (18)
Sequential Steps in the Assembly of the Multimeric Outer Membrane Secretin PulD* (2013) (18)
Bacterial Secretins Form Constitutively Open Pores Akin to General Porins (2013) (18)
A simple technique for the differentiation of Escherichia coli in water examination (1973) (17)
The Haloprotease CPI Produced by the Moderately Halophilic Bacterium Pseudoalteromonas ruthenica Is Secreted by the Type II Secretion Pathway (2009) (15)
Chromosomal location of the attachment site for the PA-2 prophage in Escherichia coli K-12 (1979) (15)
Outer Membrane Proteins of Escherichia coli (15)
The immunity and lysis genes of ColN plasmid pCHAP4 (1988) (15)
Pullulanase: Model protein substrate for the general secretory pathway of gram-negative bacteria (2008) (15)
Colicin E2 production and release by Escherichia coli K12 and other Enterobacteriaceae. (1985) (14)
A Single Amino Acid Substitution Changes the Self-Assembly Status of a Type IV Piliation Secretin (2012) (14)
OmpF porin synthesis in Escherichia coli strains B and K-12 carrying heterologous ompB and/or ompF loci (1983) (14)
Autoinduced synthesis of colicin E2 (2004) (14)
Klebsiella pneumoniae strain K21: evidence for the rapid secretion of an unacylated form of pullulanase (1989) (13)
Independent domain assembly in a trapped folding intermediate of multimeric outer membrane secretins. (2014) (13)
Lipids assist the membrane insertion of a BAM-independent outer membrane protein (2015) (13)
Coliphage which requires either the LamB protein or the OmpC protein for adsorption to Escherichia coli K-12 (1981) (13)
Colicin N forms voltage- and pH-dependent channels in planar lipid bilayer membranes (2006) (13)
beta-Galactosidase and alkaline phosphatase do not become extracellular when fused to the amino-terminal part of colicin N. (1986) (12)
The targeting, docking and anti-proteolysis functions of the secretin chaperone PulS. (2013) (11)
Prepore Stability Controls Productive Folding of the BAM-independent Multimeric Outer Membrane Secretin PulD* (2016) (11)
Traffic spotting: poles apart (2004) (11)
Colicin E4-CT9 is proteolytically degraded after discharge from producing cells in liquid cultures. (1983) (10)
A fluorescent antibody technique for the enumeration of faecal streptococci in water. (1975) (10)
CHAPTER II – Basic principles and techniques (1989) (9)
On the effect of ompR on colicin E2 production (1983) (9)
A new regulatory locus of the maltose regulon in Klebsiella pneumoniae strain K21 identified by the study of pullulanase secretion mutants. (1989) (9)
CHAPTER IX – Applications of protein targeting (1989) (8)
CHAPTER III – Early stages in the secretory pathway (1989) (8)
Transcription regulation of colicin Ib synthesis (2004) (7)
Map location of the cbr gene coding for production of the outer membrane receptor for ferrienterochelin and colicins B and D in Escherichia coli K-12 (1977) (7)
Gene encoding a hybrid OmpF-PhoE pore protein in the outer membrane of Escherichia coli K12 (2004) (7)
Outer membrane translocation of the extracellular enzyme pullulanase in Escherichia coli K12 does not require a fatty acylated N-terminal cysteine. (1991) (7)
Yersinia spp. HMWP2, a cytosolic protein with a cryptic internal signal sequence which can promote alkaline phosphatase export (1995) (7)
On remaining cytoplasmic. (1990) (6)
The Use of Hybrid Proteins in the Study of Protein Targeting Signals (1988) (6)
Structural investigations of outer membrane proteins from Escherichia coli. (1982) (6)
Immunofluorescence identification of faecal streptococci using commercially-available antisera (1974) (6)
The two-component response regulator PprB modulates quorum-sensing signal production and global gene expression in Pseudomonas aeruginosa. (2008) (6)
Protein Traffic in Bacteria (1998) (6)
A mutation in the dsbA gene coding for periplasmic disulfide oxidoreductase reduces transcription of the Escherichia coli ompF gene (1993) (6)
HrcN IS A PERIPHERAL MEMBRANE ATPASE THAT IS ACTIVATED BY OLIGOMERIZATION (2003) (4)
Transformation by electroporation (1988) (4)
Bacterial toxins deliver the goods. (1996) (3)
A kit generation (1990) (3)
Investigation of the regulation of the Escherichia coli btuB gene using operon fusions. (1986) (3)
Regulated OmpF porin synthesis in Escherichia coli K-12 (1983) (3)
CHAPTER IV – Later stages in the prokaryotic secretory pathway (1989) (2)
Genes affecting the major outer membrane proteins of Escherichia coli K-12: Mutations at nmpA and nmpB (2004) (2)
Pullulanase: A New Specific Secretion Pathway in Escherichia Coli (1988) (2)
CHAPTER VII – The targeting of nuclear proteins (1989) (2)
Identification ofThreeGenesControlling Production ofNew OuterMembranePoreProteins inEscherichia coli K-12 (1978) (2)
Not as simple as ABC. (1988) (2)
Immunofluorescence as a method for the detection of Escherichia coli in water (1974) (2)
CHAPTER VI – The targeting of mitochondrial, chloroplast, and peroxisomal proteins (1989) (1)
Lysosomes and vacuoles. (1987) (1)
Characterization andExpression oftheStructural Genefor Pullulanase, a Maltose-Inducible Secreted Protein of (1985) (1)
Later stages in the eukaryotic secretory pathway (1989) (1)
If you'll pardon the expression. (1987) (1)
Why study Escherichia coli? (2012) (1)
Multiple Pathways of Exoprotein Secretion in Gram-Negative Bacteria (2001) (0)
Livres reçusExtracellular enzymes of microorganisms: Chaloupka, J. & Krumphazil, V. 1 vol. (17 × 25,5 cm), 216 + ix pages. Plenum Press, New York, London, 1987. (1988) (0)
Imrnunofluorescence as a method for the detection of Escherichia coli in water1 (1974) (0)
An age-old old age problem. (1986) (0)
Genetic approaches to the study of protein targeting (1990) (0)
The NAT L. Sternberg Thesis Prize (2001) (0)
Genetic instability in streptomycetes. (1987) (0)
Lighten our darkness. (1987) (0)
One hundred years of Pasteur and his Institute. (1987) (0)
Commentary Bacterial toxins deliver the goods (2005) (0)
Progress towards the identification of secretion signals in a protein transported in a folded state across a lipid bilayer (1996) (0)
Two new pepsin proteases of microbial origin. (1988) (0)
Low resolution structure of two components of the type II pullulanase secretion machinery of Klebsiella oxytoca (2005) (0)
Subcellular organelle biogenesis: turning protein export outside-in. (1986) (0)
Crystal structure of the type 2 secretion system pilotin from Klebsiella Oxytoca (2011) (0)
Attractive propositions: microbial responses to chemical signals. (1987) (0)
Taking toxins to pieces. (1987) (0)
Lipoprotein Sorting in Escherichia Coli (1998) (0)
Crystal structure of N-terminal truncated pseudopilin PulG (2004) (0)
CHAPTER VIII – Endocytosis (1989) (0)
Changing the message. (1987) (0)
Editorial Announcement (2022) (0)
New rules for regulation (4)--Recent advances in the study of transcription attenuation. (1987) (0)
New rules for regulation. (5). Proteases as regulatory proteins. (1987) (0)
Release ofColicin E2fromEscherichia coli (1981) (0)
Editorial (2008) (0)
Some like it hot. (1986) (0)
BLA BLA and TET à TET. (1986) (0)
Quicker progress on slow diseases. (1986) (0)
Transposons are not hopping mad. (1986) (0)
Crystal structure of PulA from Klebsiella oxytoca (2013) (0)
The 8th International Biotechnology Symposium, Paris, July 17-22, 1988 (1988) (0)
Editorial (2008) (0)
Gram -ve bacteria secrete proteins via an extended general export pathway (1990) (0)
CHAPTER I – An overview of protein targeting (1989) (0)
A GeneforaNewLipoprotein inthedapA-purC Interval ofthe Escherichia coli Chromosome (1991) (0)
MicroMeeting Getting out : protein traffic in prokaryotes (2004) (0)
Greasy proteins. (1988) (0)
Comparison ofColicins B-K260 andD-CA23: Purification and Characterization oftheColicins andExamination ofColicin Immunity intheProducing Strains (1977) (0)
The Structure, Genesis and Dynamics of Biological Membranes (1992) (0)

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