Andreas Plückthun
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Why Is Andreas Plückthun Influential?
(Suggest an Edit or Addition)According to Wikipedia, Andreas Plückthun is a scientist whose research is focused on the field of protein engineering. Andreas Plückthun is the director of the department of biochemistry at the University of Zurich. Plückthun was appointed to the faculty of the University of Zurich as a Full professor of biochemistry in 1993. Plückthun was group leader at the Max Planck Institute of Biochemistry , Germany . He was elected to the European Molecular Biology Organization in 1992, and named a member of the German National Academy of Science in 2003. He is cofounder of the biotechnology companies Morphosys Molecular Partners AG and G7 Therapeutics. .
Andreas Plückthun's Published Works
Number of citations in a given year to any of this author's works
Total number of citations to an author for the works they published in a given year. This highlights publication of the most important work(s) by the author
Published Works
- In vitro selection and evolution of functional proteins by using ribosome display. (1997) (1011)
- Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. (1988) (968)
- Induction and Exhaustion of Lymphocytic Choriomeningitis Virus–specific Cytotoxic T Lymphocytes Visualized Using Soluble Tetrameric Major Histocompatibility Complex Class I–Peptide Complexes (1998) (775)
- Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides. (2000) (733)
- Engineering novel binding proteins from nonimmunoglobulin domains (2005) (636)
- High-affinity binders selected from designed ankyrin repeat protein libraries (2004) (630)
- Stability engineering of antibody single-chain Fv fragments. (2001) (573)
- In vitro generated antibodies specific for telomeric guanine-quadruplex DNA react with Stylonychia lemnae macronuclei (2001) (571)
- Reproducibility: Standardize antibodies used in research (2015) (510)
- Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins. (2003) (509)
- Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. (1997) (508)
- A comparison of strategies to stabilize immunoglobulin Fv-fragments. (1990) (463)
- Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display (2000) (359)
- New protein engineering approaches to multivalent and bispecific antibody fragments. (1997) (328)
- Biophysical properties of human antibody variable domains. (2003) (325)
- In-vitro protein evolution by ribosome display and mRNA display. (2004) (324)
- BIACORE analysis of histidine-tagged proteins using a chelating NTA sensor chip. (1997) (320)
- Sequence statistics reliably predict stabilizing mutations in a protein domain. (1994) (317)
- Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy. (2015) (312)
- Miniantibodies: use of amphipathic helices to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli. (1992) (302)
- Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries. (1998) (293)
- Competition BIAcore for measuring true affinities: large differences from values determined from binding kinetics. (1996) (280)
- Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target (2007) (276)
- Stable one-step technetium-99m labeling of His-tagged recombinant proteins with a novel Tc(I)–carbonyl complex (1999) (275)
- Designed to be stable: Crystal structure of a consensus ankyrin repeat protein (2003) (273)
- A label-free immunosensor array using single-chain antibody fragments. (2005) (264)
- Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments (1998) (260)
- Domain interactions in the Fab fragment: a comparative evaluation of the single-chain Fv and Fab format engineered with variable domains of different stability. (2005) (258)
- Antibody scFv fragments without disulfide bonds made by molecular evolution. (1998) (255)
- Engineered turns of a recombinant antibody improve its in vivo folding. (1995) (242)
- ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome (2007) (241)
- Disrupting the hydrophobic patches at the antibody variable/constant domain interface: improved in vivo folding and physical characterization of an engineered scFv fragment. (1997) (239)
- The Functional Expression of Antibody Fv Fragments in Ischhuchia coli: Improved Vectors and a Generally Applicable Purification Technique (1991) (237)
- A designed ankyrin repeat protein evolved to picomolar affinity to Her2. (2007) (236)
- Antigen binding forces of individually addressed single-chain Fv antibody molecules. (1998) (233)
- Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis. (1994) (231)
- Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display. (2008) (230)
- Tailoring in vitro evolution for protein affinity or stability. (2001) (224)
- Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular size. (2010) (221)
- Acyl and phosphoryl migration in lysophospholipids: importance in phospholipid synthesis and phospholipase specificity. (1982) (219)
- Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering. (2004) (217)
- DARPins and other repeat protein scaffolds: advances in engineering and applications. (2011) (216)
- High thermal stability is essential for tumor targeting of antibody fragments: engineering of a humanized anti-epithelial glycoprotein-2 (epithelial cell adhesion molecule) single-chain Fv fragment. (1999) (215)
- Functional and dynamic polymerization of the ALS-linked protein TDP-43 antagonizes its pathologic aggregation (2017) (208)
- Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display (2006) (204)
- Selection for a periplasmic factor improving phage display and functional periplasmic expression (1998) (204)
- Yet another numbering scheme for immunoglobulin variable domains: an automatic modeling and analysis tool. (2001) (204)
- Selection for improved protein stability by phage display. (1999) (203)
- The disulfide bonds in antibody variable domains: effects on stability, folding in vitro, and functional expression in Escherichia coli. (1992) (198)
- Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli (2014) (198)
- Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells (2015) (197)
- The Escherichia coli heat shock proteins GroEL and GroES modulate the folding of the beta‐lactamase precursor. (1990) (196)
- Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity (2008) (192)
- Ribosome display: an in vitro method for selection and evolution of antibodies from libraries. (1999) (192)
- Selecting proteins with improved stability by a phage-based method (1998) (192)
- Protein folding in the periplasm of Escherichia coli (1994) (191)
- Directed in Vitro Evolution and Crystallographic Analysis of a Peptide-binding Single Chain Antibody Fragment (scFv) with Low Picomolar Affinity* (2004) (183)
- Covalently circularized nanodiscs for studying membrane proteins and viral entry (2016) (180)
- Design of multivalent complexes using the barnase·barstar module (2003) (180)
- Functional antibody production using cell-free translation: Effects of protein disulfide isomerase and chaperones (1997) (175)
- Folding and unfolding mechanism of highly stable full-consensus ankyrin repeat proteins. (2008) (174)
- In vitro display technologies: novel developments and applications. (2001) (171)
- Filamentous phage infection: crystal structure of g3p in complex with its coreceptor, the C-terminal domain of TolA. (1999) (170)
- Biophysical properties of camelid V(HH) domains compared to those of human V(H)3 domains. (2002) (169)
- An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments. (1994) (165)
- The Periplasmic Escherichia coli Peptidylprolyl cis,trans-Isomerase FkpA (2000) (162)
- High volumetric yields of functional dimeric miniantibodies in Escherichia coli, using an optimized expression vector and high-cell-density fermentation under non-limited growth conditions (1996) (160)
- Factors influencing the dimer to monomer transition of an antibody single-chain Fv fragment. (1998) (160)
- Improving in vivo folding and stability of a single-chain Fv antibody fragment by loop grafting. (1997) (158)
- Circular β‐lactamase: stability enhancement by cyclizing the backbone (1999) (157)
- The Periplasmic Escherichia coli Peptidylprolyl cis,trans-Isomerase FkpA (2000) (154)
- PIP2 stabilises active states of GPCRs and enhances the selectivity of G-protein coupling (2018) (153)
- Recent advances in producing and selecting functional proteins by using cell-free translation. (1998) (151)
- Direct identification of ligand-receptor interactions on living cells and tissues (2012) (146)
- Engineered proteins as specific binding reagents. (2005) (143)
- A novel strategy to design binding molecules harnessing the modular nature of repeat proteins (2003) (142)
- Structure of a kinesin–tubulin complex and implications for kinesin motility (2013) (141)
- Model and simulation of multivalent binding to fixed ligands. (1998) (140)
- Destabilization of the complete protein secondary structure on binding to the chaperone GroEL (1994) (137)
- Correlation between in Vitro Stability and in Vivo Performance of Anti-GCN4 Intrabodies as Cytoplasmic Inhibitors* (2000) (136)
- Different equilibrium stability behavior of ScFv fragments: identification, classification, and improvement by protein engineering. (1999) (136)
- Cyclic Green Fluorescent Protein Produced in Vivo Using an Artificially Split PI-PfuI Intein from Pyrococcus furiosus * (2001) (135)
- Crystal Structure of the Dimeric C-terminal Domain of TonB Reveals a Novel Fold* (2001) (135)
- Tetravalent miniantibodies with high avidity assembling in Escherichia coli. (1995) (134)
- Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism. (2007) (133)
- An Escherichia coli protein consisting of a domain homologous to FK506-binding proteins (FKBP) and a new metal binding motif. (1994) (131)
- The Escherichia coli SlyD Is a Metal Ion-regulated Peptidyl-prolyl cis/trans-Isomerase* (1997) (130)
- Antibody Engineering: Advances From the Use of Escherichia coli Expression Systems (1991) (130)
- Mono‐ and Bivalent Antibody Fragments Produced in Escherichia coli: Engineering, Folding and Antigen Binding (1992) (130)
- A natural antibody missing a cysteine in VH: consequences for thermodynamic stability and folding. (1997) (130)
- Designing repeat proteins: modular leucine-rich repeat protein libraries based on the mammalian ribonuclease inhibitor family. (2003) (129)
- Expression of functional antibody Fv and Fab fragments in Escherichia coli. (1989) (129)
- Improved Bivalent Miniantibodies, with Identical Avidity as Whole Antibodies, Produced by High Cell Density Fermentation of Escherichia coli (1993) (128)
- Intracellular Kinase Inhibitors Selected from Combinatorial Libraries of Designed Ankyrin Repeat Proteins* (2005) (128)
- Mutual stabilization of VL and VH in single-chain antibody fragments, investigated with mutants engineered for stability. (1998) (127)
- A recombinant immunotoxin derived from a humanized epithelial cell adhesion molecule-specific single-chain antibody fragment has potent and selective antitumor activity. (2003) (127)
- Antigen recognition by conformational selection (1999) (126)
- A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end (2012) (126)
- Docking small ligands in flexible binding sites (1998) (125)
- Protein PEGylation Decreases Observed Target Association Rates via a Dual Blocking Mechanisms⃞ (2005) (125)
- An intrinsically stable antibody scFv fragment can tolerate the loss of both disulfide bonds and fold correctly (1998) (122)
- Beyond binding: using phage display to select for structure, folding and enzymatic activity in proteins. (1999) (121)
- Designed ankyrin repeat proteins (DARPins) from research to therapy. (2012) (121)
- Designed armadillo repeat proteins as general peptide-binding scaffolds: consensus design and computational optimization of the hydrophobic core. (2008) (120)
- The structural basis of phage display elucidated by the crystal structure of the N-terminal domains of g3p (1998) (114)
- Selection and Characterization of Her2 Binding-designed Ankyrin Repeat Proteins* (2006) (110)
- DARPins recognizing the tumor-associated antigen EpCAM selected by phage and ribosome display and engineered for multivalency. (2011) (110)
- Ribosome display: a perspective. (2012) (110)
- PEGylation and Multimerization of the Anti-p185HER-2 Single Chain Fv Fragment 4D5 (2006) (110)
- Reprogramming Bacteriophage Host Range through Structure-Guided Design of Chimeric Receptor Binding Proteins. (2019) (109)
- The Effect of Folding Catalysts on the In Vivo Folding Process of Different Antibody Fragments Expressed in Escherichia coli (1993) (106)
- Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. (1997) (106)
- DARPins: an efficient targeting domain for lentiviral vectors. (2011) (106)
- Structural basis for eliciting a cytotoxic effect in HER2-overexpressing cancer cells via binding to the extracellular domain of HER2. (2013) (105)
- Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach. (2003) (104)
- Selecting and evolving functional proteins in vitro by ribosome display. (2000) (103)
- Effects of overexpressing folding modulators on the in vivo folding of heterologous proteins in Escherichia coli. (1995) (102)
- A heterodimeric coiled-coil peptide pair selected in vivo from a designed library-versus-library ensemble. (2000) (102)
- Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation. (2006) (99)
- Bispecific Designed Ankyrin Repeat Proteins (DARPins) Targeting Epidermal Growth Factor Receptor Inhibit A431 Cell Proliferation and Receptor Recycling* (2011) (98)
- Engineered proteins with desired specificity: DARPins, other alternative scaffolds and bispecific IgGs. (2014) (98)
- Direct in Vivo Screening of Intrabody Libraries Constructed on a Highly Stable Single-chain Framework* (2002) (96)
- Secretion and in vivo folding of the Fab fragment of the antibody McPC603 in Escherichia coli: influence of disulphides and cis-prolines. (1991) (95)
- Consensus Design of Repeat Proteins (2004) (95)
- Tumor Targeting of Mono-, Di-, and Tetravalent Anti-p185HER-2 Miniantibodies Multimerized by Self-associating Peptides* (2001) (92)
- Antibodies from Escherichia coli (1990) (92)
- Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries (2012) (92)
- Multivalent antibody fragments with high functional affinity for a tumor-associated carbohydrate antigen. (1996) (90)
- Antitumor activity of an epithelial cell adhesion molecule–targeted nanovesicular drug delivery system (2007) (90)
- Novel fold and capsid-binding properties of the λ-phage display platform protein gpD (2000) (90)
- Epithelial cell adhesion molecule-targeted drug delivery for cancer therapy (2013) (87)
- Functional antibody single-chain fragments from the cytoplasm of Escherichia coli: influence of thioredoxin reductase (TrxB). (1995) (85)
- Affinity-matured recombinant antibody fragments analyzed by single-molecule force spectroscopy. (2007) (85)
- Characterization of the linker peptide of the single‐chain Fv fragment of an antibody by NMR spectroscopy (1993) (85)
- Off-target-free gene delivery by affinity-purified receptor-targeted viral vectors (2015) (85)
- In vitro selection for catalytic activity with ribosome display. (2002) (83)
- Novel fold and capsid-binding properties of the lambda-phage display platform protein gpD. (2000) (83)
- Activation, aggregation, and product inhibition of cobra venom phospholipase A2 and comparison with other phospholipases. (1985) (83)
- From DARPins to LoopDARPins: novel LoopDARPin design allows the selection of low picomolar binders in a single round of ribosome display. (2013) (83)
- Stepwise unfolding of ankyrin repeats in a single protein revealed by atomic force microscopy. (2006) (82)
- Stabilizing membrane proteins through protein engineering. (2013) (82)
- Tandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins--evaluation of two anti-His-tag monoclonal antibodies. (1998) (82)
- Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations (2012) (81)
- A Novel Fusion Toxin Derived from an EpCAM-Specific Designed Ankyrin Repeat Protein Has Potent Antitumor Activity (2010) (81)
- EpCAM-targeted delivery of nanocomplexed siRNA to tumor cells with designed ankyrin repeat proteins (2009) (80)
- High-resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist (2018) (79)
- The precursor of beta‐lactamase: purification, properties and folding kinetics. (1989) (79)
- The first constant domain (CH1 and CL) of an antibody used as heterodimerization domain for bispecific miniantibodies (1998) (79)
- Co‐selection of cognate antibody‐antigen pairs by selectively‐infective phages (1995) (78)
- Inclusion of an upstream transcriptional terminator in phage display vectors abolishes background expression of toxic fusions with coat protein g3p. (1996) (78)
- An in vivo library-versus-library selection of optimized protein–protein interactions (1999) (77)
- Evolution of three human GPCRs for higher expression and stability. (2011) (77)
- Adenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control (2018) (77)
- Allosteric inhibition of aminoglycoside phosphotransferase by a designed ankyrin repeat protein. (2005) (77)
- Correctly folded T-cell receptor fragments in the periplasm of Escherichia coli. Influence of folding catalysts. (1994) (77)
- Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library (2003) (75)
- Development of the designed ankyrin repeat protein (DARPin) G3 for HER2 molecular imaging (2014) (75)
- A dimeric bispecific miniantibody combines two specificities with avidity (1998) (74)
- Characterization and further stabilization of designed ankyrin repeat proteins by combining molecular dynamics simulations and experiments. (2008) (74)
- Atomic force microscopy detects changes in the interaction forces between GroEL and substrate proteins. (1998) (74)
- High enzymatic activity and chaperone function are mechanistically related features of the dimeric E. coli peptidyl-prolyl-isomerase FkpA. (2001) (74)
- Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters (2013) (70)
- Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells. (2013) (70)
- DARPin-targeting of measles virus: unique bispecificity, effective oncolysis, and enhanced safety. (2013) (70)
- Rapid selection of specific MAP kinase-binders from designed ankyrin repeat protein libraries. (2006) (69)
- Glycine to alanine substitutions in helices of glyceraldehyde-3-phosphate dehydrogenase: effects on stability. (1990) (68)
- Thermodynamic partitioning model for hydrophobic binding of polypeptides by GroEL. I. GroEL recognizes the signal sequences of beta-lactamase precursor. (1994) (67)
- PtdIns(4,5)P2 stabilizes active states of GPCRs and enhances selectivity of G-protein coupling (2018) (66)
- The importance of framework residues H6, H7 and H10 in antibody heavy chains: experimental evidence for a new structural subclassification of antibody V(H) domains. (2001) (65)
- Helix-stabilized Fv (hsFv) antibody fragments: substituting the constant domains of a Fab fragment for a heterodimeric coiled-coil domain. (2001) (65)
- Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module. (2010) (64)
- Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding (2016) (64)
- The influence of the framework core residues on the biophysical properties of immunoglobulin heavy chain variable domains. (2009) (63)
- Stabilizing ionic interactions in a full-consensus ankyrin repeat protein. (2008) (62)
- Her2-specific multivalent adapters confer designed tropism to adenovirus for gene targeting. (2011) (61)
- Designed ankyrin repeat proteins (DARPins) as novel isoform-specific intracellular inhibitors of c-Jun N-terminal kinases. (2012) (61)
- Efficient cell-specific uptake of binding proteins into the cytoplasm through engineered modular transport systems. (2015) (61)
- Ribosome display: a technology for selecting and evolving proteins from large libraries. (2011) (60)
- Refined crystal structure of a recombinant immunoglobulin domain and a complementarity-determining region 1-grafted mutant. (1993) (59)
- Rapid selection of high-affinity binders using ribosome display. (2012) (59)
- In vitro selection and characterization of DARPins and Fab fragments for the co-crystallization of membrane proteins: The Na(+)-citrate symporter CitS as an example. (2007) (59)
- Domain interactions in antibody Fv and scFv fragments: effects on unfolding kinetics and equilibria (1999) (59)
- Direct selection of antibodies from complex libraries with the protein fragment complementation assay. (2006) (58)
- Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry (2014) (58)
- Selection based on the folding properties of proteins with ribosome display (2003) (58)
- Comparison of Escherichia coli and rabbit reticulocyte ribosome display systems (1999) (58)
- Comparison of in vivo selection and rational design of heterodimeric coiled coils. (2002) (58)
- Fast selection of antibodies without antigen purification: adaptation of the protein fragment complementation assay to select antigen-antibody pairs. (2001) (58)
- Thermodynamic partitioning model for hydrophobic binding of polypeptides by GroEL. II. GroEL recognizes thermally unfolded mature beta-lactamase. (1994) (57)
- Facile double-functionalization of designed ankyrin repeat proteins using click and thiol chemistries. (2012) (57)
- Removal of the conserved disulfide bridges from the scFv fragment of an antibody: effects on folding kinetics and aggregation. (1999) (57)
- Mapping and modification of an antibody hapten binding site: a site-directed mutagenesis study of McPC603. (1991) (57)
- Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution. (2012) (57)
- Lung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor (2018) (54)
- Receptor-Targeted Nipah Virus Glycoproteins Improve Cell-Type Selective Gene Delivery and Reveal a Preference for Membrane-Proximal Cell Attachment (2016) (54)
- In vitro selection and evolution of proteins. (2000) (54)
- Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution. (2010) (54)
- Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts (2016) (53)
- A quantitative comparison of cytosolic delivery via different protein uptake systems (2017) (53)
- The influence of the buried glutamine or glutamate residue in position 6 on the structure of immunoglobulin variable domains. (2001) (53)
- The ErbB4 CYT2 variant protects EGFR from ligand-induced degradation to enhance cancer cell motility (2014) (52)
- Modular peptide binding: from a comparison of natural binders to designed armadillo repeat proteins. (2014) (52)
- Getting to reproducible antibodies: the rationale for sequenced recombinant characterized reagents. (2015) (51)
- Cryo-EM structure of an activated GPCR–G protein complex in lipid nanodiscs (2020) (50)
- An antibody library for stabilizing and crystallizing membrane proteins – selecting binders to the citrate carrier CitS (2004) (50)
- The consequences of stepwise deletions from the signal-processing site of beta-lactamase. (1987) (50)
- Folding and assembly of an antibody Fv fragment, a heterodimer stabilized by antigen. (1999) (50)
- Orthogonal assembly of a designed ankyrin repeat protein-cytotoxin conjugate with a clickable serum albumin module for half-life extension. (2013) (49)
- Knowledge-based design of a biosensor to quantify localized ERK activation in living cells. (2013) (49)
- Viral escape at the molecular level explained by quantitative T-cell receptor/peptide/MHC interactions and the crystal structure of a peptide/MHC complex. (2000) (49)
- Stabilization and humanization of a single-chain Fv antibody fragment specific for human lymphocyte antigen CD19 by designed point mutations and CDR-grafting onto a human framework. (2009) (49)
- Crystal Structure and Function of a DARPin Neutralizing Inhibitor of Lactococcal Phage TP901-1 (2009) (48)
- Crystal structures of the human neurokinin 1 receptor in complex with clinically used antagonists (2019) (47)
- Designed ankyrin repeat proteins: a novel tool for testing epidermal growth factor receptor 2 expression in breast cancer (2010) (47)
- Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly (2001) (47)
- The INNs and outs of antibody nonproprietary names (2015) (46)
- Circular beta-lactamase: stability enhancement by cyclizing the backbone. (1999) (45)
- Isolation of Intracellular Proteinase Inhibitors Derived from Designed Ankyrin Repeat Proteins by Genetic Screening* (2006) (45)
- Comprehensive analysis of heterotrimeric G-protein complex diversity and their interactions with GPCRs in solution (2015) (44)
- Liposome functionalization with copper-free "click chemistry". (2015) (44)
- Engineering aggregation resistance in IgG by two independent mechanisms: lessons from comparison of Pichia pastoris and mammalian cell expression. (2012) (44)
- Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen. (2002) (43)
- Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5 (2005) (43)
- Folding of a designed simple ankyrin repeat protein (2004) (43)
- Selection, characterization and x-ray structure of anti-ampicillin single-chain Fv fragments from phage-displayed murine antibody libraries. (2001) (42)
- Forces and energetics of hapten-antibody dissociation: a biased molecular dynamics simulation study. (2001) (42)
- Antibody-Drug Conjugates for Tumor Targeting-Novel Conjugation Chemistries and the Promise of non-IgG Binding Proteins. (2015) (42)
- Chemosensitization of carcinoma cells using epithelial cell adhesion molecule–targeted liposomal antisense against bcl-2/bcl-xL (2006) (41)
- Structural and dynamic properties of the Fv fragment and the single-chain Fv fragment of an antibody in solution investigated by heteronuclear three-dimensional NMR spectroscopy. (1994) (41)
- Designed Armadillo repeat proteins: library generation, characterization and selection of peptide binders with high specificity. (2012) (41)
- Transfer of engineered biophysical properties between different antibody formats and expression systems. (2012) (41)
- Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity (2017) (40)
- Kinetic stability and crystal structure of the viral capsid protein SHP. (2004) (39)
- Transmission electron microscopy of GroEL, GroES, and the symmetrical GroEL/ES complex. (1994) (39)
- Selectively infective phages (SIP). (1997) (39)
- The rate‐limiting steps for the folding of an antibody scFv fragment (1997) (39)
- Self-immobilizing recombinant antibody fragments for immunoaffinity chromatography: generic, parallel, and scalable protein purification. (2002) (38)
- Selection and characterization of DARPins specific for the neurotensin receptor 1. (2009) (38)
- Non-repetitive single-chain Fv linkers selected by selectively infective phage (SIP) technology. (1998) (38)
- Intermolecular biparatopic trapping of ErbB2 prevents compensatory activation of PI3K/AKT via RAS–p110 crosstalk (2016) (38)
- Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation. (2013) (38)
- beta-Lactamase binds to GroEL in a conformation highly protected against hydrogen/deuterium exchange. (1996) (38)
- Animal-free alternatives and the antibody iceberg (2020) (37)
- Cleavage of procaryotically expressed human immunodeficiency virus fusion proteins by factor Xa and application in western blot (immunoblot) assays (1989) (37)
- Trapped! A Critical Evaluation of Methods for Measuring Total Cellular Uptake versus Cytosolic Localization. (2019) (37)
- Selectively infective phage (SIP) technology: scope and limitations. (1999) (37)
- Crystal structure of a consensus‐designed ankyrin repeat protein: Implications for stability (2006) (36)
- Folding nuclei of the scFv fragment of an antibody. (1996) (36)
- The nature of antibody heavy chain residue H6 strongly influences the stability of a VH domain lacking the disulfide bridge. (1998) (36)
- Flavonol‐mediated stabilization of PIN efflux complexes regulates polar auxin transport (2020) (36)
- Engineering Af1521 improves ADP-ribose binding and identification of ADP-ribosylated proteins (2020) (36)
- Folding, heterodimeric association and specific peptide recognition of a murine alphabeta T-cell receptor expressed in Escherichia coli. (1999) (35)
- Monovalent single‐chain Fv fragments and bivalent miniantibodies bound to vesicular stomatitis virus protect against lethal infection (1996) (35)
- The hierarchy of mutations influencing the folding of antibody domains in Escherichia coli. (1999) (35)
- Optimization of designed armadillo repeat proteins by molecular dynamics simulations and NMR spectroscopy (2012) (35)
- Conformation-Dependent Recognition of HIV gp120 by Designed Ankyrin Repeat Proteins Provides Access to Novel HIV Entry Inhibitors (2013) (35)
- Improving the apo-state detergent stability of NTS1 with CHESS for pharmacological and structural studies. (2014) (34)
- Increasing the Antitumor Effect of an EpCAM-Targeting Fusion Toxin by Facile Click PEGylation (2013) (34)
- Secretion of heterologous proteins in Escherichia coli. (1990) (34)
- Structure‐based optimization of designed Armadillo‐repeat proteins (2012) (34)
- Identification of a functional epitope of the Nogo receptor by a combinatorial approach using ribosome display. (2005) (34)
- Residue-resolved stability of full-consensus ankyrin repeat proteins probed by NMR. (2010) (34)
- Modern Antibody Technology: The Impact on Drug Development (2008) (32)
- The scFv fragment of the antibody hu4D5-8: evidence for early premature domain interaction in refolding. (2001) (32)
- Reproducing the natural evolution of protein structural features with the selectively infective phage (SIP) technology. The kink in the first strand of antibody kappa domains. (1998) (32)
- Selectively infective phage (SIP) technology: A novel method for in vivo selection of interacting protein–ligand pairs (1997) (32)
- Multispecific Targeting with Synthetic Ankyrin Repeat Motif Chimeric Antigen Receptors (2019) (31)
- Role of monomeric activators in cobra venom phospholipase A2 action. (1982) (30)
- GroE prevents the accumulation of early folding intermediates of pre-beta-lactamase without changing the folding pathway. (1992) (30)
- Affinity and folding properties both influence the selection of antibodies with the selectively infective phage (SIP) methodology (1997) (30)
- Short-chain phosphatidylethanolamines: physical properties and susceptibility of the monomers to phospholipase A2 action. (1985) (29)
- Two-dimensional crystals of the molecular chaperone GroEL reveal structural plasticity. (1993) (29)
- A structural model of a Ras–Raf signalosome (2020) (28)
- Crystallization and preliminary X-ray studies of the VL domain of the antibody McPC603 produced in Escherichia coli. (1990) (28)
- Systemic analysis of tyrosine kinase signaling reveals a common adaptive response program in a HER2-positive breast cancer (2019) (28)
- A kinetic trap is an intrinsic feature in the folding pathway of single-chain Fv fragments. (2002) (27)
- Rigidly connected multispecific artificial binders with adjustable geometries (2017) (27)
- Signal sequence mutants of beta-lactamase. (1985) (27)
- Changes to International Nonproprietary Names for antibody therapeutics 2017 and beyond: of mice, men and more (2017) (27)
- Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin (2016) (27)
- Structural changes of the Escherichia coli GroEL-GroES chaperonins upon complex formation in solution: a neutron small angle scattering study. (1998) (27)
- Multiple cycles of global unfolding of GroEL-bound cyclophilin A evidenced by NMR. (1997) (27)
- DARPin-Based Crystallization Chaperones Exploit Molecular Geometry as a Screening Dimension in Protein Crystallography. (2016) (27)
- Strategies for the expression of antibody fragments in (1991) (26)
- The CHASE domain: a predicted ligand-binding module in plant cytokinin receptors and other eukaryotic and bacterial receptors (2001) (26)
- Facile promoter deletion in Escherichia coli in response to leaky expression of very robust and benign proteins from common expression vectors (2009) (26)
- Construction and characterization of protein libraries composed of secondary structure modules (2002) (25)
- A Library-Based Screening Strategy for the Identification of DARPins as Ligands for Receptor-Targeted AAV and Lentiviral Vectors (2018) (25)
- Structure and Energetic Contributions of a Designed Modular Peptide-Binding Protein with Picomolar Affinity. (2016) (25)
- Assessment of ab initio models of protein complexes by molecular dynamics (2018) (25)
- Phase behavior of a designed cyclopropyl analogue of monoolein: implications for low-temperature membrane protein crystallization. (2015) (25)
- CHAPTER 14 – Phosphorus-31 NMR of Phospholipids in Micelles (1984) (24)
- Ligand binding of a ribosome-displayed protein detected in solution at the single molecule level by fluorescence correlation spectroscopy (2002) (24)
- Crystal structure and function of a DARPin neutralizing inhibitor of lactococcal phage TP901-1: comparison of DARPin and camelid VHH binding mode (2010) (24)
- A versatile and highly repressible Escherichia coli expression system based on invertible promoters: expression of a gene encoding a toxic product. (1993) (23)
- Engineering of antibodies with a known three-dimensional structure. (1987) (23)
- Complexes of the neurotensin receptor 1 with small-molecule ligands reveal structural determinants of full, partial, and inverse agonism (2021) (23)
- Optimizing the anti-tumor efficacy of protein-drug conjugates by engineering the molecular size and half-life. (2020) (23)
- Folding in vitro and transport in vivo of pre‐β‐lactamase are SecB independent (1991) (23)
- Antibodies: validate recombinants once (2015) (23)
- Ribosome display of mammalian receptor domains. (2005) (23)
- A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution (2016) (23)
- Knowledge-based design of reagentless fluorescent biosensors from a designed ankyrin repeat protein. (2010) (22)
- Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools. (2016) (22)
- Hemolytic assay for venom phospholipase A2. (1981) (22)
- Comparison of the amide proton exchange behavior of the rapidly formed folding intermediate and the native state of an antibody scFv fragment (1997) (22)
- Ligand Discovery for a Peptide-Binding GPCR by Structure-Based Screening of Fragment- and Lead-Like Chemical Libraries. (2017) (21)
- Direct screening for phosphatase activity by turnover-based capture of protein catalysts. (2002) (21)
- Structural Model for the Interaction of a Designed Ankyrin Repeat Protein with the Human Epidermal Growth Factor Receptor 2 (2013) (21)
- Design and Characterization of Modular Scaffolds for Tubulin Assembly* (2012) (21)
- Influence of size and charge of unstructured polypeptides on pharmacokinetics and biodistribution of targeted fusion proteins. (2019) (21)
- Amyloid-β Peptide-specific DARPins as a Novel Class of Potential Therapeutics for Alzheimer Disease* (2014) (21)
- NMR solution structure of the monomeric form of the bacteriophage λ capsid stabilizing protein gpD (2005) (21)
- Receptor-targeted lentiviral vectors are exceptionally sensitive toward the biophysical properties of the displayed single-chain Fv. (2015) (21)
- Zooming in on the hydrophobic ridge of H-2D(b): implications for the conformational variability of bound peptides. (2001) (20)
- Direct Screening for Phosphatase Activity by Turnover-Based Capture of Protein Catalysts This work was supported by BBSRC studentships (to J.B. and H.D.), by an EPSRC studentship (to J.H.R.), and by an EC TMR studentship (to S.C.-T.). (2002) (20)
- Novel prodrug-like fusion toxin with protease-sensitive bioorthogonal PEGylation for tumor targeting. (2014) (20)
- A mutation designed to alter crystal packing permits structural analysis of a tight‐binding fluorescein–scFv complex (2005) (20)
- Identification of the binding surface on beta-lactamase for GroEL by limited proteolysis and MALDI-mass spectrometry. (1998) (20)
- SPR-based fragment screening with neurotensin receptor 1 generates novel small molecule ligands (2017) (20)
- Determinants of Ligand Subtype-Selectivity at α1A-Adrenoceptor Revealed Using Saturation Transfer Difference (STD) NMR. (2018) (20)
- Animal-derived-antibody generation faces strict reform in accordance with European Union policy on animal use (2020) (19)
- AFM structural study of the molecular chaperone GroEL and its two-dimensional crystals: an ideal "living" calibration sample. (2002) (19)
- Individual filamentous phage imaged by electron holography (2011) (19)
- Production of recombinant human beta2-microglobulin for scintigraphic diagnosis of amyloidosis in uremia and hemodialysis. (2000) (19)
- Two conformational states of beta-lactamase bound to GroEL: a biophysical characterization. (1998) (19)
- Parallel pathways in the folding of a short-term denatured scFv fragment of an antibody. (1998) (18)
- In vitro selection methods for screening of peptide and protein libraries. (1999) (18)
- Directed evolution of G-protein-coupled receptors for high functional expression and detergent stability. (2013) (18)
- Enhanced lysis by bispecific oncolytic measles viruses simultaneously using HER2/neu or EpCAM as target receptors (2016) (18)
- Escherichia coli producing recombinant antibodies. (1994) (18)
- Generation of ordered protein assemblies using rigid three-body fusion (2020) (18)
- Rate-determining step in phospholipase A2 mechanism. 18O isotope exchange determined by 13C NMR. (1986) (17)
- Chaperone-assisted structure elucidation with DARPins. (2020) (17)
- Spontaneous self-assembly of engineered armadillo repeat protein fragments into a folded structure. (2014) (17)
- Computationally Designed Armadillo Repeat Proteins for Modular Peptide Recognition. (2016) (17)
- Improving Expression of scFv Fragments by Co-expression of Periplasmic Chaperones (2001) (17)
- Construction and characterization of a kappa opioid receptor devoid of all free cysteines. (2004) (16)
- Molecular dynamics study of the stabilities of consensus designed ankyrin repeat proteins (2006) (16)
- G-quadruplexes are specifically recognized and distinguished by selected designed ankyrin repeat proteins (2014) (16)
- GroEL walks the fine line: the subtle balance of substrate and co-chaperonin binding by GroEL. A combinatorial investigation by design, selection and screening. (2006) (16)
- A cleavable ligand column for the rapid isolation of large quantities of homogeneous and functional neurotensin receptor 1 variants from E. coli. (2015) (16)
- Characterizing the functionality of recombinant T-cell receptors in vitro: a pMHC tetramer based approach. (2000) (16)
- The Antibody Society’s antibody validation webinar series (2020) (16)
- Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation (2018) (16)
- Imaging the native structure of the chaperone protein GroEL without fixation using atomic force microscopy (2001) (16)
- Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W. (2014) (15)
- iMATCH: an integrated modular assembly system for therapeutic combination high-capacity adenovirus gene therapy (2021) (15)
- Crystal structures of designed armadillo repeat proteins: Implications of construct design and crystallization conditions on overall structure (2014) (14)
- Ribosome Display: In Vitro Selection of Protein-Protein Interactions (2006) (14)
- Reproducibility: bypass animals for antibody production (2020) (14)
- Engineering an anti-HER2 biparatopic antibody with a multimodal mechanism of action (2021) (14)
- Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc (2020) (14)
- Protein-fold evolution in the test tube. (2001) (14)
- Effect of single point mutations in citrate synthase on binding to GroEL (1996) (14)
- Personalised proteome analysis by means of protein microarrays made from individual patient samples (2017) (14)
- Insight into microtubule nucleation from tubulin-capping proteins (2019) (13)
- High-Throughput Quantification of Surface Protein Internalization and Degradation. (2019) (13)
- Advances in the design and engineering of peptide-binding repeat proteins (2017) (13)
- Structures of neurokinin 1 receptor in complex with Gq and Gs proteins reveal substance P binding mode and unique activation features (2021) (13)
- Analysis of IgG kinetic stability by differential scanning calorimetry, probe fluorescence and light scattering (2017) (13)
- Inhibition of the MET Kinase Activity and Cell Growth in MET-Addicted Cancer Cells by Bi-Paratopic Linking. (2019) (13)
- Combinatorial Approaches To Novel Proteins (2004) (13)
- Probing the Conformation States of Neurotensin Receptor 1 Variants by NMR Site‐Directed Methyl Labeling (2020) (12)
- Cobra venom phospholipase A2: A review of its action toward lipid/water interfaces (1981) (12)
- Direct evidence by H/D exchange and ESI‐MS for transient unproductive domain interaction in the refolding of an antibody scFv fragment (2008) (12)
- Advanced analyses of kinetic stabilities of iggs modified by mutations and glycosylation (2015) (12)
- Curvature of designed armadillo repeat proteins allows modular peptide binding. (2017) (12)
- SPRi-MALDI MS: characterization and identification of a kinase from cell lysate by specific interaction with different designed ankyrin repeat proteins (2017) (11)
- The SHREAD gene therapy platform for paracrine delivery improves tumor localization and intratumoral effects of a clinical antibody (2021) (11)
- Structural Basis for the Selective Inhibition of c-Jun N-Terminal Kinase 1 Determined by Rigid DARPin-DARPin Fusions. (2017) (11)
- Corrigendum: ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome (2007) (11)
- Structural features of the McPC603 Fab fragment not defined in the X‐ray structure (1990) (11)
- Optimizing the α1B-adrenergic receptor for solution NMR studies. (2020) (11)
- Electron Microscopy of the GroEL-GroES Filament (1995) (11)
- Rigidity of the extracellular part of HER2: Evidence from engineering subdomain interfaces and shared‐helix DARPin‐DARPin fusions (2017) (11)
- Engineering and functional immobilization of opioid receptors. (2005) (11)
- Rigid fusions of designed helical repeat binding proteins efficiently protect a binding surface from crystal contacts (2019) (11)
- Unravelling Receptor and RGD Motif Dependence of Retargeted Adenoviral Vectors using Advanced Tumor Model Systems (2019) (11)
- Comparison of the Fv Fragments of Different Phosphorylcholine Binding Antibodies Expressed in Escherichia coli (1991) (10)
- Modification of the kinetic stability of immunoglobulin G by solvent additives (2018) (10)
- Structural analysis of biological targets by host:guest crystal lattice engineering (2019) (10)
- An Interface-Driven Design Strategy Yields a Novel, Corrugated Protein Architecture. (2018) (10)
- Lactoferrin-Hexon Interactions Mediate CAR-Independent Adenovirus Infection of Human Respiratory Cells (2020) (10)
- Structures of designed armadillo-repeat proteins show propagation of inter-repeat interface effects (2016) (10)
- Strategies for Selection from Protein Libraries Composed of de Novo Designed Secondary Structure Modules (2004) (10)
- High-resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist (2018) (9)
- Folding intermediates of β‐lactamase recognized by GroEL (1997) (9)
- Recombinant Antibodies for Immunotherapy: Alternative Scaffolds: Expanding the Options of Antibodies (2009) (9)
- HIV: Antibodies with a split personality (2010) (9)
- Structures of designed armadillo repeat proteins binding to peptides fused to globular domains (2017) (9)
- Improving the apostate detergent stability of NTS 1 with CHESS for pharmacological and structural studies (2014) (9)
- Targeted delivery and endosomal cellular uptake of DARPin-siRNA bioconjugates: Influence of linker stability on gene silencing. (2019) (9)
- Rotational symmetry of the structured Chip/LDB-SSDP core module of the Wnt enhanceosome (2019) (8)
- PIN-bodies: a new class of antibody-like proteins with CD4 specificity derived from the protein inhibitor of neuronal nitric oxide synthase. (2006) (8)
- The Rationality of Random Screening—Efficient Methods of Selection of Peptides and Oligonucleotide Ligands (1991) (8)
- NK cells with tissue-resident traits shape response to immunotherapy by inducing adaptive antitumor immunity (2022) (8)
- Structure of cyclized green fluorescent protein. (2002) (8)
- Crystal structures of the human neurokinin 1 receptor in complex with clinically used antagonists (2019) (8)
- Peptide-Guided Assembly of Repeat Protein Fragments. (2018) (8)
- The membrane attack complex of complement and its precursor proteins lack phospholipase activity. (1983) (8)
- Malignant tissues produce divergent antibody glycosylation of relevance for cancer gene therapy effectiveness (2020) (8)
- Insight into odorant perception: the crystal structure and binding characteristics of antibody fragments directed against the musk odorant traseolide. (1999) (8)
- High-Throughput Fluorescence Polarization Assay to Identify Ligands Using Purified G Protein-Coupled Receptor (2019) (8)
- The design of evolution and the evolution of design (2007) (7)
- Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation (2020) (7)
- DARPins recognize mTFP1 as novel reagents for in vitro and in vivo protein manipulations (2018) (7)
- A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteins. (2017) (7)
- A combined NMR and computational approach to investigate peptide binding to a designed Armadillo repeat protein. (2015) (7)
- A Mouse Ig κ Domain of Very Unusual Framework Structure Loses Function when Converted to the Consensus (*) (1995) (7)
- Directed Evolution with Fast and Efficient Selection Technologies (2001) (7)
- Directed evolution for high functional production and stability of a challenging G protein-coupled receptor (2021) (7)
- A Universal Approach to Prepare Reagents for DNA-Assisted Protein Analysis (2014) (7)
- Structure-guided design of a peptide lock for modular peptide binders. (2020) (7)
- Membrane-bound beta-lactamase forms in Escherichia coli. (1988) (7)
- Animal- versus in vitro-derived antibodies: avoiding the extremes (2021) (7)
- Selectively infective phage technology. (2000) (7)
- High-Throughput Generation of Bispecific Binding Proteins by Sortase A–Mediated Coupling for Direct Functional Screening in Cell Culture (2019) (6)
- Mutations in sigma 70 transcription factor improves expression of functional eukaryotic membrane proteins in Escherichia coli (2019) (6)
- Crystal structure of the α1B-adrenergic receptor reveals molecular determinants of selective ligand recognition (2022) (6)
- International nonproprietary names for monoclonal antibodies: an evolving nomenclature system (2022) (6)
- Wie rational ist Zufalls‐Screening? ‐ Effiziente Methoden der Selektion von Peptid‐ und Oligonucleotid‐Liganden (1991) (6)
- Apoptosis-inducing anti-HER2 agents operate through oligomerization-induced receptor immobilization (2021) (6)
- Facile Site-Specific Multiconjugation Strategies in Recombinant Proteins Produced in Bacteria. (2019) (6)
- In vivo assembly and large-scale purification of a GPCR - Gα fusion with Gβγ, and characterization of the active complex (2019) (6)
- Structural basis of adenylyl cyclase 9 activation (2021) (6)
- A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins (2019) (6)
- Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol (2020) (6)
- Pharmacokinetic properties of bivalent miniantibodies and comparison to other immunoglobulin forms (1995) (6)
- Crystal structure of a truncated version of the phage λ protein gpD (2004) (6)
- Reengineering anthrax toxin protective antigen for improved receptor-specific protein delivery (2020) (6)
- Erratum to “In-vitro protein evolution by ribosome display and mRNA display” [J. Immunol. Methods. 290 (2003) 51–67] (2004) (5)
- Monovalent antibody scFv fragments selected to modulate T-cell activation by inhibition of CD86-CD28 interaction. (2007) (5)
- An Approach for the Real-Time Quantification of Cytosolic Protein-Protein Interactions in Living Cells. (2021) (5)
- Distinct conformations of the HIV-1 V3 loop crown are targetable for broad neutralization (2021) (5)
- Structure of the recombinant antibody Fab fragment f3p4. (2008) (4)
- Modulation of the Kinetic Stability of Immunoglobulin G by Solvent Additives (2016) (4)
- An automated iterative approach for protein structure refinement using pseudocontact shifts (2021) (4)
- Programmable DARPin-based receptors for the detection of thrombotic markers (2022) (4)
- A Library-Based Screening Strategy for the Identification of DARPins as Ligands for Receptor-Targeted AAV and Lentiviral Vectors (2021) (4)
- Antibody engineering. (1991) (4)
- Mechanism of Interaction of Phospholipase A2 with Phospholipid Substrates and Activators (1986) (4)
- Engineering single pan-specific ubiquibodies for targeted degradation of all forms of endogenous ERK protein kinase (2021) (4)
- Site-Selective Enzymatic Labeling of Designed Ankyrin Repeat Proteins Using Protein Farnesyltransferase. (2019) (4)
- Engineering Proteins for Stability and Efficient Folding (2008) (4)
- FORCE MICROSCOPY OF CELLS TO MEASURE BIOADHESION (1999) (4)
- Rate-determining Step in Phospholipase A2 Mechanism (1986) (3)
- Peptide binding affinity redistributes preassembled repeat protein fragments (2018) (3)
- Wege zu neuen Enzymen: Protein Engineering und Katalytische Antikörper (1990) (3)
- Letter to the Editor: Backbone HN, N, Cα, C′ and Cβ chemical shift assignments and secondary structure of FkpA, a 245-residue peptidyl-prolyl cis/trans isomerase with chaperone activity (2004) (3)
- Antibody engineering and therapeutics conference (2014) (3)
- Computational Modeling of Designed Ankyrin Repeat Protein Complexes with Their Targets. (2019) (3)
- Purification of MBP fusion proteins using engineered DARPin affinity matrix. (2021) (3)
- Rapid Selection of High-Affinity Antibody scFv Fragments Using Ribosome Display. (2018) (3)
- Modular peptide binders – development of a predictive technology as alternative for reagent antibodies (2022) (3)
- Disrupting the HDAC6-ubiquitin interaction impairs infection by influenza and Zika virus and cellular stress pathways (2022) (3)
- Crystal structure of a truncated version of the phage lambda protein gpD. (2004) (3)
- Engineering Integral Membrane Proteins for Expression and Stability (2011) (3)
- Half-life extension of efficiently produced DARPin serum albumin fusions as a function of FcRn affinity and recycling. (2021) (3)
- New views into class B GPCRs from the crystal structure of PTH1R (2019) (3)
- Engineering of Challenging G Protein-Coupled Receptors for Structure Determination and Biophysical Studies (2021) (3)
- Synthetic Antibodies with a Known Three-Dimensional Structure (1988) (2)
- Proteomics' framework for success? (2007) (2)
- The RGD-binding integrins αvβ6 and αvβ8 are receptors for mouse adenovirus-1 and -3 infection (2021) (2)
- Cover Picture: Consensus Design of Repeat Proteins (ChemBioChem 2/2004) (2004) (2)
- Passive and catalytic antibodies and drug delivery (2004) (2)
- Antibody Engineering and Therapeutics Conference (2013) (2)
- Structural basis for the activation and ligand recognition of the human oxytocin receptor (2022) (2)
- Antibody Engineering to Study Protein-Ligand Interactions and Catalysis: The Phosphorylcholine Binding Antibodies (1993) (2)
- Properties of FV and Fab fragments of the antibody McPC603 expressed in E. coli. (1990) (2)
- Erratum to “Designed Armadillo Repeat Proteins: Library Generation, Characterization and Selection of Peptide Binders with High Specificity” [J. Mol. Biol. (2012) 424, 68–87] (2013) (2)
- Thermodynamic Stability Is a Strong Predictor for the Delivery of DARPins to the Cytosol via Anthrax Toxin (2021) (2)
- Letter to the Editor: Assignments of 1H and 15N resonances of the bacteriophage λ capsid stabilizing protein gpD (2004) (2)
- Studying Protein Structure and Function by Directed Evolution (1998) (2)
- Modular Adapters Utilizing Binders of Different Molecular Types Expand Cell-Targeting Options for Adenovirus Gene Delivery. (2022) (1)
- Universal platform for the generation of thermostabilized GPCRs that crystallize in LCP (2022) (1)
- Conformational changes and spatial arrangement of the E. coli chaperones GroEL and GroES (1997) (1)
- Biotechnological aspects of antibody production in E. coli (1991) (1)
- Conformation of fatty acyl chains in α- and β-phosphatidylcholine and phosphatidylethanolamine derivatives in sonicated vesicles (1986) (1)
- Conformation of fatty acyl chains in alpha- and beta-phosphatidylcholine and phosphatidylethanolamine derivatives in sonicated vesicles. (1986) (1)
- Thermal Shift Assay for Small GTPase Stability Screening: Evaluation and Suitability (2022) (1)
- Folding in vitro and transport in vivo of pre-beta-lactamase are SecB independent. (1991) (1)
- Folding intermediates of beta-lactamase recognized by GroEL. (1997) (1)
- A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology (2022) (1)
- Designed Ankyrin Repeat Proteins as a tool box for analyzing p63 (2022) (1)
- Labeling surface proteins with high specificity: Intrinsic limitations of phosphopantetheinyl transferase systems (2019) (1)
- Correction: DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations (doi:10.1242/bio.036749) (2018) (1)
- T-cell receptor signal sequences. (1995) (1)
- Catalytic antibodies: contributions from engineering and expression in Escherichia coli. (1991) (1)
- Kirchhammer et al. Successful IL-12 cancer immunotherapy requires NK cell-derived CCL5 for anti-tumor DC-T cell crosstalk (2021) (1)
- Designed Ankyrin Repeat Proteins as Novel Binders for Ultrasound Molecular Imaging. (2021) (1)
- Antibody reproducibility challenges: the solution lies in the sequence (2016) (1)
- Improved Repeat Protein Stability by Combined Consensus and Computational Protein Design. (2022) (1)
- BACTERIOPHAGE LAMBDA HEAD PROTEIN D (2000) (0)
- fi c uptake of binding proteins into the cytoplasm through engineered modular transport systems (2015) (0)
- EngBF DARPin Fusion 9b 3G124 (2019) (0)
- Designed Armadillo Repeat protein Lock1 bound to (KR)4KLSF target (2020) (0)
- Mutations in sigma 70 transcription factor improves expression of functional eukaryotic membrane proteins in Escherichia coli (2019) (0)
- A Vaccinia-based system for directed evolution of GPCRs in mammalian cells (2022) (0)
- Designed Armadillo repeat proteins serve as scaffolds for the rational assembly of peptide binders with picomolar affinities (2015) (0)
- CHITIN BINDING DOMAINS FOR IMMOBILIZING ANTIBODY FRAGMENTS IN IMMUNAFFINITY CHROMATOGRAPHY (2002) (0)
- Designed Ankyrin Repeat Proteins for detecting prostate-specific antigen expression in vivo (2023) (0)
- Process for the preparation of genetically engineered antibodies. (1988) (0)
- Recombinant antibodies produced in E. coli : prospects for catalysis (1990) (0)
- Method for the stabilization of the hybrid immunoglobulins or immunoglobulin fragments and anti-EGP-2 scFv fragments stabilized. (2000) (0)
- Structural Analysis of a Tight-binding Fluorescein-scFv; apo form (2005) (0)
- Bispezifische HER2-Liganden zur Krebstherapie (2012) (0)
- Le ribosome display (2001) (0)
- Sortase-Mediated Site-Specific Conjugation and 89Zr-Radiolabeling of Designed Ankyrin Repeat Proteins for PET. (2022) (0)
- DARPins against the amyloid-beta petide selected via ribosome display (2010) (0)
- New developments in combinatorial libraries. (1997) (0)
- Targeting Oncolytic Adenoviruses to Cancer Cells Using a Designed Ankyrin Repeat Protein (DARPin) Lipocalin-2 (LCN2) Fusion Protein. (2023) (0)
- Capsid stabilizing protein GPD, NMR, 20 Structures (2005) (0)
- Three-Dimensional Structure Of a Single Chain Fv Fragment Complexed With The peptide GCN4(7P-14P). (2004) (0)
- Abstract A75: Designed Ankyrin Repeat Proteins (DARPins) as recognition motifs in chimeric antigen receptors (2017) (0)
- Crystal structures of HER3 extracellular domain 4 in complex with the designed ankyrin-repeat protein D5 (2021) (0)
- Paracrine delivery of therapeutic biologics for cancer (2019) (0)
- Escherichia coil Producing Recombinant Aodtodies (2020) (0)
- Crystal structure of the α1B-adrenergic receptor reveals molecular determinants of selective ligand recognition (2022) (0)
- Reengineering anthrax toxin protective antigen for improved receptor-specific protein delivery (2020) (0)
- Crystal structure of Aminoglycoside Phosphotransferase APH(3')-IIIa in complex with the inhibitor AR_3a (2005) (0)
- Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity (2017) (0)
- Nuclear myosin VI maintains replication fork stability (2022) (0)
- Abstract 2126: Development of a precision chordoma research program (2019) (0)
- An in vivo "library-to-library-selektion" of optimized protein-protein interactions (2000) (0)
- Editorial overview: Protein design and evolution-new protein architectures, evolutionary fine-tuning and analysis. (2015) (0)
- Author Correction: Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc (2022) (0)
- Three-dimensional structure of anti-ampicillin single chain Fv fragment from phage-displayed murine antibody libraries (2001) (0)
- Rigidly connected multispecific artificial binders with adjustable geometries (2017) (0)
- Symposium 3: Non-enzymatic biocatalysts in nature and biotechnology (1990) (0)
- Backbone HN, N, C alpha, C' and C beta chemical shift assignments and secondary structure of FkpA, a 245-residue peptidyl-prolyl cis/trans isomerase with chaperone activity. (2004) (0)
- Reproducibility: bypass animals for antibody production (2020) (0)
- MAX randomisation: designed, non-degenerate saturation mutagenesis of armadillo repeat proteins. (2018) (0)
- Adenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control (2018) (0)
- Biofunctional nanodot arrays in living cells uncover synergistic co-condensation of Wnt signalodroplets (2022) (0)
- Dimerization domain of Xenopus laevis LDB1 in complex with darpin 10 (2019) (0)
- Design and application of crystallization aids comprising DARPin domains (2017) (0)
- Ultraschall, ein Tätigkeitsfeld für MTRA? Das Berliner Modell in Theorie und Praxis (2005) (0)
- Ligand binding of a ribosome-displayed protein detected in solution at the single molecule level by fluorescence correlation spectroscopy (2002) (0)
- Designed armadillo repeat protein with 4 internal repeats, 2nd generation C-cap and 3rd generation N-cap. (2016) (0)
- Crystal structure of anti-mTFP1 DARPin 1238_E11 (2018) (0)
- A process for the detection of nucleic acid molecules encoding (poly) peptides that interact with target molecules (1998) (0)
- A quantitative comparison of cytosolic delivery via different protein uptake systems (2017) (0)
- Crystal structure of the Neurokinin 1 receptor in complex with the small molecule antagonist CP-99,994 (2019) (0)
- Crystal structure of a designed selected Ankyrin Repeat protein in complex with the MAP kinase ERK2 (2012) (0)
- Phage display by co-translational translocation fusion polypeptide. (2006) (0)
- FAP-retargeted Ad5 enables in vivo gene delivery to stromal cells in the tumor microenvironment (2022) (0)
- Crystal Structure of scFv against peptide GCN4 (2004) (0)
- Functional and dynamic polymerization of the ALS-linked protein TDP-43 antagonizes its pathologic aggregation (2017) (0)
- DARPins recognizing mTFP1 as novel reagents forin vitroandin vivoprotein manipulations. (2018) (0)
- Three-dimensional structure of anti-ampicillin single chain Fv fragment complexed with the hapten. (2001) (0)
- Crystal Structure Of The LCMV Peptidic Epitope Gp276 In Complex With The Murine Class I Mhc Molecule H-2Db (2001) (0)
- Beyond antibodies: advances from engineered binding proteins (2012) (0)
- Adaptive anti-tumor immunity is orchestrated by a population of CCL5-producing tissue-resident NK cells (2021) (0)
- Modification of Adenovirus Receptor Specificity by Designed Multivalent Targeting Adapters (2010) (0)
- Inhibition of the Cell Growth in M Cells by Bi-Para (2019) (0)
- BBRC PIN-bodies : A new class of antibody-like proteins with CD 4 specificity derived from the protein inhibitor of neuronal nitric oxide synthase q (2006) (0)
- Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation (2018) (0)
- ParaMAX: non-degenerative saturation mutagenesis applied to an alpha helical repeat protein (2019) (0)
- Saturation mutagenesis and precision libraries: the numbers game (2019) (0)
- Selection and evaluation of designed ankyrin repeat proteins (DARPins) reactive with carcinoembryonic antigen (CEA) (2007) (0)
- PRe-ART: Predictive Reagent Antibody Replacement Technology (2019) (0)
- A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins (2019) (0)
- Crystal structure of a mycobacterial hsp60 epitope with the murine class I MHC molecule H-2Db (2003) (0)
- Structure-based engineering of designed armadillo repeat proteins. (2014) (0)
- Crystal structure of a designed selected Ankyrin Repeat protein in complex with the phosphorylated MAP kinase ERK2 (2012) (0)
- Structural basis of adenylyl cyclase 9 activation (2022) (0)
- Designed Armadillo repeat protein (YIIIM3AIII) (2012) (0)
- s6.m20.o5 Designed Ankyrin Repeat Protein Libraries: A Valuable new Tool for the Crystallization of Biological (2004) (0)
- Abstract B27: A DARPin-based toolbox to understand and treat RAS-addicted cancers (2020) (0)
- Abstract 3912: Pre-clinical developments of the G3 Designed ankyrin repeat protein (DARPin) forinvivoassessment of HER2 expression . (2013) (0)
- Correction to: An automated iterative approach for protein structure refinement using pseudocontact shifts (2021) (0)
- Enzymatic Construction of DARPin-Based Targeted Delivery Systems Using Protein Farnesyltransferase and a Capture and Release Strategy (2022) (0)
- Combinatorial and Evolutionary Protein Engineering (2007) (0)
- Author Correction: Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc (2022) (0)
- Targeted adenovirus-mediated transduction of human T cells in vitro and in vivo. (2023) (0)
- Gerichtete evolution von membranproteinen in eukaryotischen zellen mit einer zellwand (2015) (0)
- Collections de protéines de répétition comportant des modules de répétition (2001) (0)
- Crystal structure of anti-mTFP1 DARPin 1238_G01 in space group P212121 (2018) (0)
- Three-dimensional structure of anti-ampicillin single chain Fv fragment. (2001) (0)
- Selection in vivo bibliotheque contre bibliotheque d'interactions proteine-proteine optimisees (2000) (0)
- Structure of a hydrophobic leucinostatin derivative determined by host lattice display. (2022) (0)
- Designed ankyrin repeat protein libraries: a valuable new tool for the crystallization of biological macromolecules (2004) (0)
- Zellspezifische Produktion von multiplen Therapeutika im Körper (2022) (0)
- Stability of engineered antibody fragments (1993) (0)
- Designed armadillo repeat protein with 5 internal repeats, 2nd generation C-cap and 3rd generation N-cap. (2016) (0)
- CRISPR-clear imaging of melanin-rich B16-derived solid tumors (2023) (0)
- Perspective: The Antibody Validation Webinar Series from The Antibody Society (2020) (0)
- Crystal structure of computationally designed armadillo repeat proteins for modular peptide recognition. (2016) (0)
- DARPIN D1 binding to tubulin beta chain (not in complex) (2013) (0)
- Crystal structure of a DARPin in complex with ORF49 from Lactococcal phage TP901-1 (2009) (0)
- Protein-Struktur und Funktion: Die Mölichkeiten und Probleme der Computersimulation aus der Sicht des Experimentators (1990) (0)
- Crystal structure of E3_19 a designed ankyrin repeat protein (2006) (0)
- CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAINS OF BACTERIOPHAGE MINOR COAT PROTEIN G3P (1998) (0)
- Molecular imaging of PSA using DARPin scaffolds (2022) (0)
- Structural analysis of mycobacterial and murine hsp60 epitopes in complex with the class I MHC molecule H‐2Db (2003) (0)
- Designed Ankyrin Repeat Proteins provide insights into the structure and function of CagI and are potent inhibitors of CagA translocation by the Helicobacter pylori type IV secretion system (2022) (0)
- Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation (2020) (0)
- CRYSTAL STRUCTURE OF AN AMPICILLIN SINGLE CHAIN FV, FORM 1, FREE (2001) (0)
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