Bernd Nidetzky

Q: What Schools Are Affiliated With Bernd Nidetzky

Bernd Nidetzky is affiliated with the following schools: ETH Zurich, Graz University of Technology, Technical University of Munich, University of Graz, University of Vienna, University of Natural Resources and Life Sciences, Vienna

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Bernd Nidetzky

Chemistry

#3136

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#4127

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#342

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#356

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Bernd Nidetzky

Biology

#8845

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#11979

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#115

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#1253

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#1298

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Biochemistry

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#1426

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Chemistry
Biology

Bernd Nidetzky's Degrees

PhD Biotechnology University of Vienna
Masters Chemical Engineering University of Vienna
Bachelors Chemical Engineering University of Vienna

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Bernd Nidetzky's Published Works

Number of citations in a given year to any of this author's works

Total number of citations to an author for the works they published in a given year. This highlights publication of the most important work(s) by the author

Published Works

Production of fungal xylanases (1996) (421)
Cellulose hydrolysis by the cellulases from Trichoderma reesei: a new model for synergistic interaction. (1994) (250)
Cellulose Surface Degradation by a Lytic Polysaccharide Monooxygenase and Its Effect on Cellulase Hydrolytic Efficiency* (2014) (207)
Metal-Organic Framework-Based Enzyme Biocomposites. (2021) (181)
Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae (2008) (142)
Advanced characterization of immobilized enzymes as heterogeneous biocatalysts (2015) (137)
Biotransformations in microstructured reactors: more than flowing with the stream? (2011) (136)
Cellulose hydrolysis by the cellulases from Trichoderma reesei: adsorptions of two cellobiohydrolases, two endocellulases and their core proteins on filter paper and their relation to hydrolysis. (1994) (135)
The coenzyme specificity of Candida tenuis xylose reductase (AKR2B5) explored by site-directed mutagenesis and X-ray crystallography. (2005) (123)
Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development. (2016) (115)
Characterization of dTDP-4-dehydrorhamnose 3,5-Epimerase and dTDP-4-dehydrorhamnose Reductase, Required for dTDP-l-rhamnose Biosynthesis in Salmonella enterica Serovar Typhimurium LT2* (1999) (112)
Variations of the 2‐His‐1‐carboxylate Theme in Mononuclear Non‐Heme FeII Oxygenases (2006) (109)
A high-yielding biocatalytic process for the production of 2-O-(alpha-D-glucopyranosyl)-sn-glycerol, a natural osmolyte and useful moisturizing ingredient. (2008) (103)
NAD(P)H-dependent aldose reductase from the xylose-assimilating yeast Candida tenuis. Isolation, characterization and biochemical properties of the enzyme. (1997) (103)
Carrier-free immobilized enzymes for biocatalysis (2010) (102)
The structure of apo and holo forms of xylose reductase, a dimeric aldo-keto reductase from Candida tenuis. (2002) (100)
Fusion to a pull‐down domain: a novel approach of producing Trigonopsis variabilisD‐amino acid oxidase as insoluble enzyme aggregates (2007) (96)
Recombinant sucrose phosphorylase from Leuconostoc mesenteroides: characterization, kinetic studies of transglucosylation, and application of immobilised enzyme for production of alpha-D-glucose 1-phosphate. (2007) (95)
Leloir Glycosyltransferases as Biocatalysts for Chemical Production (2018) (95)
Continuous enzymatic production of xylitol with simultaneous coenzyme regeneration in a charged membrane reactor (2000) (91)
A new approach for modeling cellulase–cellulose adsorption and the kinetics of the enzymatic hydrolysis of microcrystalline cellulose (1993) (90)
Carbohydrate synthesis by disaccharide phosphorylases: Reactions, catalytic mechanisms and application in the glycosciences (2010) (89)
Nutritional requirements of the BY series of Saccharomyces cerevisiae strains for optimum growth. (2012) (88)
Induction of Mannanase, Xylanase, and Endoglucanase Activities in Sclerotium rolfsii (1998) (87)
Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization (2010) (85)
A simple assay for measuring cellobiose dehydrogenase activity in the presence of laccase. (1999) (84)
Leloir Glycosyltransferases and Natural Product Glycosylation: Biocatalytic Synthesis of the C-Glucoside Nothofagin, a Major Antioxidant of Redbush Herbal Tea (2013) (78)
Reaction coordinate analysis for beta-diketone cleavage by the non-heme Fe2+-dependent dioxygenase Dke1. (2005) (75)
Oxidation of Monolignols by Members of the Berberine Bridge Enzyme Family Suggests a Role in Plant Cell Wall Metabolism* (2015) (72)
Single-molecule study of oxidative enzymatic deconstruction of cellulose (2017) (71)
Structure of xylose reductase bound to NAD+ and the basis for single and dual co-substrate specificity in family 2 aldo-keto reductases. (2003) (69)
Positively charged mini-protein Zbasic2 as a highly efficient silica binding module: opportunities for enzyme immobilization on unmodified silica supports. (2012) (68)
Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell conversion systems. (2016) (68)
Mannitol metabolism in brown algae involves a new phosphatase family. (2014) (66)
Hydrolysis of cellooligosaccharides by Trichoderma reesei cellobiohydrolases: Experimental data and kinetic modeling (1994) (66)
Characterization of trehalose phosphorylase from Schizophyllum commune. (1999) (65)
Sucrose phosphorylase: a powerful transglucosylation catalyst for synthesis of α-D-glucosides as industrial fine chemicals (2010) (64)
Creating a Water‐Soluble Resveratrol‐Based Antioxidant by Site‐Selective Enzymatic Glucosylation (2015) (64)
Continuous enzymatic production of lactobionic acid using glucose-fructose oxidoreductase in an ultrafiltration membrane reactor (1997) (64)
Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose. I. The properties of two thermostable beta-glycosidases. (1999) (60)
Limitations in Xylose-Fermenting Saccharomyces cerevisiae, Made Evident through Comprehensive Metabolite Profiling and Thermodynamic Analysis (2010) (58)
Rules for biocatalyst and reaction engineering to implement effective, NAD(P)H-dependent, whole cell bioreductions (2015) (58)
Glucosylglycerol and glucosylglycerate as enzyme stabilizers (2010) (57)
Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose: II. Oligosaccharide formation by two thermostable beta-glycosidases. (2000) (56)
Production of a lactose-free galacto-oligosaccharide mixture by using selective enzymatic oxidation of lactose into lactobionic acid (2001) (56)
Switching between O- and C-glycosyltransferase through exchange of active-site motifs. (2012) (56)
Specific quantification of trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase–cellulose binding studies (1994) (56)
Renewal of the air-water interface as a critical system parameter of protein stability: aggregation of the human growth hormone and its prevention by surface-active compounds. (2013) (56)
UDP-glucose dehydrogenase: structure and function of a potential drug target. (2010) (54)
Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst (2010) (54)
Influence of ionic liquid cosolvent on transgalactosylation reactions catalyzed by thermostable β‐glycosylhydrolase CelB from Pyrococcus Furiosus (2006) (54)
Structure and Mechanism of Human UDP-glucose 6-Dehydrogenase (2011) (52)
Transgalactosylation by thermostable β-glycosidases from Pyrococcus furiosus and Sulfolobus solfataricus (2000) (52)
Whole-cell bioreduction of aromatic α-keto esters using Candida tenuis xylose reductase and Candida boidinii formate dehydrogenase co-expressed in Escherichia coli (2008) (51)
Visualizing cellulase activity (2013) (50)
Coated‐wall microreactor for continuous biocatalytic transformations using immobilized enzymes (2009) (50)
Role of non-covalent enzyme-substrate interactions in the reaction catalysed by cellobiose phosphorylase from Cellulomonas uda. (2000) (49)
The Cetus Process Revisited: A Novel Enzymatic Alternative for the Production of Aldose-Free D-Fructose (1998) (49)
Single-Site Oxidation, Cysteine 108 to Cysteine Sulfinic Acid, in d-Amino Acid Oxidase from Trigonopsis variabilis and Its Structural and Functional Consequences (2005) (49)
Catalytic mechanism and substrate selectivity of aldo‐keto reductases: Insights from structure‐function studies of Candida tenuis xylose reductase (2006) (48)
Kinetic study of the catalytic mechanism of mannitol dehydrogenase from Pseudomonas fluorescens. (1999) (48)
Dissecting and Reconstructing Synergism (2012) (47)
Oriented and selective enzyme immobilization on functionalized silica carrier using the cationic binding module Zbasic2: Design of a heterogeneous D‐amino acid oxidase catalyst on porous glass (2012) (47)
A pH-controlled fed-batch process can overcome inhibition by formate in NADH-dependent enzymatic reductions using formate dehydrogenase-catalyzed coenzyme regeneration. (1998) (47)
Biotechnology for Biofuels from Lignocellulosic Feedstock (2012) (47)
Electronic substituent effects on the cleavage specificity of a non-heme Fe(2+)-dependent beta-diketone dioxygenase and their mechanistic implications. (2004) (47)
The influence of feedstock characteristics on enzyme production in Trichoderma reesei: a review on productivity, gene regulation and secretion profiles (2019) (46)
Intraparticle concentration gradients for substrate and acidic product in immobilized cephalosporin C amidase and their dependencies on carrier characteristics and reaction parameters (2010) (46)
Structural and functional properties of a yeast xylitol dehydrogenase, a Zn2+-containing metalloenzyme similar to medium-chain sorbitol dehydrogenases. (1998) (45)
D-Xylose metabolism by Candida intermedia: isolation and characterisation of two forms of aldose reductase with different coenzyme specificities. (2000) (45)
Bacterial α-glucan phosphorylases (1999) (45)
Analysis and prediction of the physiological effects of altered coenzyme specificity in xylose reductase and xylitol dehydrogenase during xylose fermentation by Saccharomyces cerevisiae (2012) (44)
A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels. (2017) (43)
Engineering Candida tenuis Xylose Reductase for Improved Utilization of NADH: Antagonistic Effects of Multiple Side Chain Replacements and Performance of Site-Directed Mutants under Simulated In Vivo Conditions (2005) (43)
From wheat straw to bioethanol: integrative analysis of a separate hydrolysis and co-fermentation process with implemented enzyme production (2015) (43)
Oriented Immobilization of Enzymes Made Fit for Applied Biocatalysis: Non‐Covalent Attachment to Anionic Supports using Zbasic2 Module (2011) (42)
alpha-1,4-D-glucan phosphorylase of gram-positive Corynebacterium callunae: isolation, biochemical properties and molecular shape of the enzyme from solution X-ray scattering. (1997) (42)
Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts (2012) (42)
Enzymatic synthesis of β‐glucosylglycerol using a continuous‐flow microreactor containing thermostable β‐glycoside hydrolase CelB immobilized on coated microchannel walls (2009) (41)
Structural and kinetic studies of induced fit in xylulose kinase from Escherichia coli. (2007) (40)
Production of glucosyl glycerol by immobilized sucrose phosphorylase: Options for enzyme fixation on a solid support and application in microscale flow format. (2017) (39)
Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts. (2013) (39)
Transient-state and steady-state kinetic studies of the mechanism of NADH-dependent aldehyde reduction catalyzed by xylose reductase from the yeast Candida tenuis. (2001) (39)
Encapsulation of Trigonopsis variabilis D‐amino acid oxidase and fast comparison of the operational stabilities of free and immobilized preparations of the enzyme (2008) (39)
Functional characterization of the native swollenin from Trichoderma reesei: study of its possible role as C1 factor of enzymatic lignocellulose conversion (2016) (39)
Engineering of a matched pair of xylose reductase and xylitol dehydrogenase for xylose fermentation by Saccharomyces cerevisiae. (2009) (38)
Surface structural dynamics of enzymatic cellulose degradation, revealed by combined kinetic and atomic force microscopy studies (2014) (38)
Microfluidic Reactor for Continuous Flow Biotransformations with Immobilized Enzymes: the Example of Lactose Hydrolysis by a Hyperthermophilic β_cont;‐Glycoside Hydrolase (2008) (38)
Acid-base catalysis in Leuconostoc mesenteroides sucrose phosphorylase probed by site-directed mutagenesis and detailed kinetic comparison of wild-type and Glu237-->Gln mutant enzymes. (2007) (37)
Mesoporous Silica Materials Labeled for Optical Oxygen Sensing and Their Application to Development of a Silica-Supported Oxidoreductase Biocatalyst (2015) (37)
Process intensification through microbial strain evolution: mixed glucose-xylose fermentation in wheat straw hydrolyzates by three generations of recombinant Saccharomyces cerevisiae (2014) (37)
Development of an ultrahigh-temperature process for the enzymatic hydrolysis of lactose. IV. Immobilization of two thermostable beta-glycosidases and optimization of a packed-bed reactor for lactose conversion. (2002) (37)
A Convenient Enzymatic Procedure for the Production of Aldose‐Free d‐Tagatose a (1998) (37)
Synergism of Trichoderma reesei cellulases while degrading different celluloses (2004) (36)
Dissecting the effect of chemical additives on the enzymatic hydrolysis of pretreated wheat straw. (2014) (36)
Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH (2012) (36)
Kinetic and CD/MCD spectroscopic studies of the atypical, three-His-ligated, non-heme Fe2+ center in diketone dioxygenase: the role of hydrophilic outer shell residues in catalysis. (2010) (36)
Towards the synthesis of glycosylated dihydrochalcone natural products using glycosyltransferase-catalysed cascade reactions (2014) (36)
Multiphase biotransformations in microstructured reactors: opportunities for biocatalytic process intensification and smart flow processing (2013) (36)
Production of a novel pyranose 2-oxidase by basidiomycete Trametes multicolor. (1998) (36)
Asp‐196 → Ala mutant of Leuconostoc mesenteroides sucrose phosphorylase exhibits altered stereochemical course and kinetic mechanism of glucosyl transfer to and from phosphate (2006) (36)
Multivalency Effects on the Immobilization of Sucrose Phosphorylase in Flow Microchannels and Their Use in the Development of a High‐Performance Biocatalytic Microreactor (2017) (35)
Bioprocess design guided by in situ substrate supply and product removal: Process intensification for synthesis of (S)-1-(2-chlorophenyl)ethanol (2012) (35)
Induction of aldose reductase and xylitol dehydrogenase activities in Candida tenuis CBS 4435. (1997) (35)
The Microenvironment in Immobilized Enzymes: Methods of Characterization and Its Role in Determining Enzyme Performance (2019) (35)
Single-step enzymatic synthesis of (R)-2-O-alpha-D-glucopyranosyl glycerate, a compatible solute from micro-organisms that functions as a protein stabiliser. (2009) (34)
Fine tuning of coenzyme specificity in family 2 aldo‐keto reductases revealed by crystal structures of the Lys‐274 → Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NAD+ and NADP+ (2005) (34)
Enzymatic Production of Pure D-Mannitol at High Productivity (1998) (34)
Unlocking the Potential of Leloir Glycosyltransferases for Applied Biocatalysis: Efficient Synthesis of Uridine 5′‐Diphosphate‐Glucose by Sucrose Synthase (2016) (34)
Identification of Candida tenuis xylose reductase as highly selective biocatalyst for the synthesis of aromatic alpha-hydroxy esters and improvement of its efficiency by protein engineering. (2007) (34)
Oriented Coimmobilization of Oxidase and Catalase on Tailor-Made Ordered Mesoporous Silica. (2017) (34)
Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1. (2016) (34)
Crystal Structure of Pseudomonas fluorescens Mannitol 2-Dehydrogenase Binary and Ternary Complexes (2002) (34)
Development of a microfluidic immobilised enzyme reactor. (2007) (33)
Effect of pretreatment severity in continuous steam explosion on enzymatic conversion of wheat straw: Evidence from kinetic analysis of hydrolysis time courses. (2016) (33)
Co-fermentation of hexose and pentose sugars in a spent sulfite liquor matrix with genetically modified Saccharomyces cerevisiae. (2013) (33)
Development of an ultrahigh-temperature process for the enzymatic hydrolysis of lactose. III. Utilization of two thermostable beta-glycosidases in a continuous ultrafiltration membrane reactor and galacto-oligosaccharide formation under steady-state conditions. (2002) (33)
Conformational flexibility related to enzyme activity: evidence for a dynamic active-site gatekeeper function of Tyr215 in Aerococcus viridans lactate oxidase (2016) (33)
Downstream processing technologies in the biocatalytic production of oligosaccharides. (2020) (32)
Quantitating intraparticle O2 gradients in solid supported enzyme immobilizates: Experimental determination of their role in limiting the catalytic effectiveness of immobilized glucose oxidase (2013) (32)
Human Enzymes for Organic Synthesis (2018) (32)
Cellobiose phosphorylase from Cellulomonas uda: gene cloning and expression in Escherichia coli, and application of the recombinant enzyme in a ‘glycosynthase-type’ reaction (2004) (31)
Probing the substrate binding site of Candida tenuis xylose reductase (AKR2B5) with site-directed mutagenesis. (2006) (31)
Cellulases Dig Deep (2011) (31)
Smart enzyme immobilization in microstructured reactors (2013) (31)
Integrated process design for biocatalytic synthesis by a Leloir Glycosyltransferase: UDP-glucose production with sucrose synthase. (2017) (31)
Let the substrate flow, not the enzyme: Practical immobilization of d‐amino acid oxidase in a glass microreactor for effective biocatalytic conversions (2016) (31)
New flavanol O-glycosides in grape and wine. (2018) (30)
Sucrose Phosphorylase Harbouring a Redesigned, Glycosyltransferase‐Like Active Site Exhibits Retaining Glucosyl Transfer in the Absence of a Covalent Intermediate (2009) (30)
Studies of the enzymic mechanism of Candida tenuis xylose reductase (AKR 2B5): X-ray structure and catalytic reaction profile for the H113A mutant. (2004) (30)
A two-step O- to C-glycosidic bond rearrangement using complementary glycosyltransferase activities. (2014) (30)
Production of hemicellulose- and cellulose-degrading enzymes by various strains of Sclerotium rolfsii. (1997) (30)
On the relationship between structure and catalytic effectiveness in solid surface-immobilized enzymes: Advances in methodology and the quest for a single-molecule perspective. (2019) (29)
Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase (2019) (29)
Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign. (2015) (29)
Enzyme identification and development of a whole‐cell biotransformation for asymmetric reduction of o‐chloroacetophenone (2011) (29)
Enzymatic hydrolysis of microcrystalline cellulose and pretreated wheat straw: a detailed comparison using convenient kinetic analysis. (2013) (29)
Structural and functional comparison of 2-His-1-carboxylate and 3-His metallocentres in non-haem iron(II)-dependent enzymes. (2008) (28)
Glycosyltransferase cascades made fit for chemical production: Integrated biocatalytic process for the natural polyphenol C‐glucoside nothofagin (2018) (28)
Process intensification for O2‐dependent enzymatic transformations in continuous single‐phase pressurized flow (2019) (28)
Characterization of a laboratory-scale container for freezing protein solutions with detailed evaluation of a freezing process simulation. (2014) (28)
Mechanistic differences among retaining disaccharide phosphorylases: insights from kinetic analysis of active site mutants of sucrose phosphorylase and alpha,alpha-trehalose phosphorylase. (2008) (28)
Biochemical characterization and mutational analysis of the mononuclear non-haem Fe2+ site in Dke1, a cupin-type dioxygenase from Acinetobacter johnsonii. (2009) (28)
Endo-β-1,4-D-mannanase is efficiently produced by Sclerotium (Athelia) rolfsii under derepressed conditions (1999) (28)
Glycosynthase Principle Transformed into Biocatalytic Process Technology: Lacto-N-triose II Production with Engineered exo-Hexosaminidase (2019) (28)
Improved operational stability of cell-free glucose-fructose oxidoreductase from Zymomonas mobilis for the efficient synthesis of sorbitol and gluconic acid in a continuous ultrafiltration membrane reactor. (1997) (28)
Purification and characterization of a laccase from the white-rot fungus Trametes multicolor. (2002) (27)
Noncovalent enzyme-substrate interactions in the catalytic mechanism of yeast aldose reductase. (1998) (27)
Simultaneous Enzymatic Synthesis of Mannitol and Gluconic Acid: II. Development of a Continuous Process for a Coupled Nad(H)-Dependent Enzyme System (1996) (27)
Trehalose phosphorylase from Pleurotus ostreatus: Characterization and stabilization by covalent modification, and application for the synthesis of α,α-trehalose (2007) (27)
Trigonopsis variabilis d-Amino Acid Oxidase: Control of Protein Quality and Opportunities for Biocatalysis through Production in Escherichia coli (2006) (27)
The role of Asp‐295 in the catalytic mechanism of Leuconostoc mesenteroides sucrose phosphorylase probed with site‐directed mutagenesis (2007) (27)
Thermal inactivation of D‐amino acid oxidase from Trigonopsis variabilis occurs via three parallel paths of irreversible denaturation (2006) (26)
Structure‐guided engineering of the coenzyme specificity of Pseudomonas fluorescens mannitol 2‐dehydrogenase to enable efficient utilization of NAD(H) and NADP(H) (2008) (25)
Biocatalytic process for production of α-glucosylglycerol using sucrose phosphorylase. (2010) (25)
β-Cyclodextrin Improves Solubility and Enzymatic C-Glucosylation of the Flavonoid Phloretin (2016) (25)
Structural and Kinetic Evidence That Catalytic Reaction of Human UDP-glucose 6-Dehydrogenase Involves Covalent Thiohemiacetal and Thioester Enzyme Intermediates* (2011) (25)
Catalytic reaction profile for NADH-dependent reduction of aromatic aldehydes by xylose reductase from Candida tenuis. (2002) (25)
Candida tenuis xylose reductase catalysed reduction of acetophenones: the effect of ring-substituents on catalytic efficiency. (2011) (25)
Bio‐based α,ω‐Functionalized Hydrocarbons from Multi‐step Reaction Sequences with Bio‐ and Metallo‐catalysts Based on the Fatty Acid Decarboxylase OleTJE (2018) (25)
Pseudomonas fluorescens mannitol 2-dehydrogenase and the family of polyol-specific long-chain dehydrogenases/reductases: sequence-based classification and analysis of structure-function relationships. (2003) (24)
In situ visualization of cooperativity among cellulases (2013) (24)
A catalytic consensus motif for D-mannitol 2-dehydrogenase, a member of a polyol-specific long-chain dehydrogenase family, revealed by kinetic characterization of site-directed mutants of the enzyme from Pseudomonas fluorescens. (2002) (24)
Cellulolytic and hemicellulolytic enzymes of sclerotium rolfsii: Optimization of the culture medium and enzymatic hydrolysis of lignocellulosic material (1994) (24)
Enzymatic hydrolysis of wheat straw after steam pretreatment: Experimental data and kinetic modelling (1993) (24)
Application of Escherichia coli maltodextrin-phosphorylase for the continuous production of glucose-1-phosphate (1995) (24)
Characterization of recombinant xylitol dehydrogenase from Galactocandida mastotermitis expressed in Escherichia coli. (2003) (24)
Biocatalytic Cascade of Polyphosphate Kinase and Sucrose Synthase for Synthesis of Nucleotide-Activated Derivatives of Glucose (2017) (23)
Comparison of Scheffersomyces stipitis strains CBS 5773 and CBS 6054 with regard to their xylose metabolism: implications for xylose fermentation (2012) (23)
Glycosynthase reaction meets the flow: Continuous synthesis of lacto‐N‐triose II by engineered β‐hexosaminidase immobilized on solid support (2020) (23)
"Short-chain" alpha-1,4-glucan phosphorylase having a truncated N-terminal domain: functional expression and characterization of the enzyme from Sulfolobus solfataricus. (2009) (23)
Characterization of a multifunctional α2,3-sialyltransferase from Pasteurella dagmatis. (2013) (23)
Enhanced Synthesis of 2-O-α-d-Glucopyranosyl-l-ascorbic Acid from α-Cyclodextrin by a Highly Disproportionating CGTase (2016) (23)
Magnetically responsive horseradish peroxidase@ZIF-8 for biocatalysis. (2019) (22)
Phosphorylase-catalyzed bottom-up synthesis of short-chain soluble cello-oligosaccharides and property-tunable cellulosic materials. (2020) (22)
Intensifying the O2-dependent heterogeneous biocatalysis: Superoxygenation of solid support from H2O2 by a catalase tailor-made for effective immobilization (2016) (22)
Multiple forms of xylose reductase in Candida intermedia: comparison of their functional properties using quantitative structure-activity relationships, steady-state kinetic analysis, and pH studies. (2003) (22)
Enzymatic Redox Cascade for One-Pot Synthesis of Uridine 5′-Diphosphate Xylose from Uridine 5′-Diphosphate Glucose (2014) (22)
Short-Chain Cello-oligosaccharides: Intensification and Scale-up of Their Enzymatic Production and Selective Growth Promotion among Probiotic Bacteria (2020) (22)
Targeting the substrate binding site of E. coli nitrile reductase QueF by modeling, substrate and enzyme engineering. (2013) (22)
In situ protein secondary structure determination in ice: Raman spectroscopy-based process analytical tool for frozen storage of biopharmaceuticals. (2014) (22)
Efficient protection of glucose-fructose oxidoreductase from Zymomonas mobilis against irreversible inactivation during its catalytic action (1995) (21)
Protein freeze concentration and micro-segregation analysed in a temperature-controlled freeze container (2015) (21)
Effect of nitrogen sources on the levels of aldose reductase and xylitol dehydrogenase activities in the xylose-fermenting yeast Candida tenuis (1998) (21)
Structure and Mechanism of Human UDP-xylose Synthase (2012) (21)
Shaking and stirring: Comparison of controlled laboratory stress conditions applied to the human growth hormone (2013) (21)
The stabilizing effects of immobilization in D-amino acid oxidase from Trigonopsis variabilis (2008) (21)
Cellular automata modeling depicts degradation of cellulosic material by a cellulase system with single-molecule resolution (2016) (21)
Shine a Light on Immobilized Enzymes: Real-time Sensing in Solid Supported Biocatalysts (2014) (20)
Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP. (2017) (20)
In situ proton NMR study of acetyl and formyl group migration in mono‐O‐acyl D‐glucose (2009) (20)
Development of a fully integrated falling film microreactor for gas–liquid–solid biotransformation with surface immobilized O2‐dependent enzyme (2016) (20)
Glycosides as compatible solutes: biosynthesis and applications. (2011) (20)
l-Lactic acid production from glucose and xylose with engineered strains of Saccharomyces cerevisiae: aeration and carbon source influence yields and productivities (2018) (20)
Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases (2019) (20)
The stereochemical course of the reaction mechanism of trehalose phosphorylase from Schizophyllum commune (1998) (20)
Three-Enzyme Phosphorylase Cascade for Integrated Production of Short-chain Cellodextrins. (2020) (19)
Novel Chemo‐Enzymatic Mimic of Hydrogen Peroxide‐Forming NAD(P)H Oxidase for Efficient Regeneration of NAD+ and NADP+ (2008) (19)
Exploring the active site of yeast xylose reductase by site‐directed mutagenesis of sequence motifs characteristic of two dehydrogenase/reductase family types (2001) (19)
Synergistic interaction of cellulases from Trichoderma reesei during cellulose degradation. (1996) (19)
Small-molecule glucosylation by sucrose phosphorylase: structure-activity relationships for acceptor substrates revisited. (2010) (19)
Catalytic mechanism of alpha-retaining glucosyl transfer by Corynebacterium callunae starch phosphorylase: the role of histidine-334 examined through kinetic characterization of site-directed mutants. (2005) (19)
Integration of enzyme, strain and reaction engineering to overcome limitations of baker's yeast in the asymmetric reduction of alpha-keto esters. (2008) (19)
Enzymatic C-glycosylation: Insights from the study of a complementary pair of plant O- and C-glucosyltransferases (2013) (19)
Hydrolysis of lactose by beta-glycosidase CelB from hyperthermophilic archaeon Pyrococcus furiosus: comparison of hollow-fiber membrane and packed-bed immobilized enzyme reactors for continuous processing of ultrahigh temperature-treated skim milk. (2002) (18)
Three‐Enzyme Phosphorylase Cascade Immobilized on Solid Support for Biocatalytic Synthesis of Cello−oligosaccharides (2020) (18)
The micromorphology of Trichoderma reesei analyzed in cultivations on lactose and solid lignocellulosic substrate, and its relationship with cellulase production (2016) (18)
Mechanistic study of CMP‐Neu5Ac hydrolysis by α2,3‐sialyltransferase from Pasteurella dagmatis (2014) (18)
Demystifying the Flow: Biocatalytic Reaction Intensification in Microstructured Enzyme Reactors. (2018) (18)
Examining the relative timing of hydrogen abstraction steps during NAD(+)-dependent oxidation of secondary alcohols catalyzed by long-chain D-mannitol dehydrogenase from Pseudomonas fluorescens using pH and kinetic isotope effects. (2002) (18)
Dissecting Physical and Biochemical Factors of Catalytic Effectiveness in Immobilized D‐Amino Acid Oxidase by Real‐Time Sensing of O2 Availability Inside Porous Carriers (2014) (18)
Characterization of recombinant Aspergillus fumigatus mannitol-1-phosphate 5-dehydrogenase and its application for the stereoselective synthesis of protio and deuterio forms of D-mannitol 1-phosphate. (2008) (18)
A tailor‐made, self‐sufficient and recyclable monooxygenase catalyst based on coimmobilized cytochrome P450 BM3 and glucose dehydrogenase (2018) (17)
Reaction of Trigonopsis variabilis d-amino acid oxidase with 2,6-dichloroindophenol: kinetic characterisation and development of an oxygen-independent assay of the enzyme activity (2005) (17)
A multistep process is responsible for product-induced inactivation of glucose-fructose oxidoreductase from Zymomonas mobilis. (1998) (17)
Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices. (2018) (17)
Selective modification of surface‐exposed thiol groups in Trigonopsis variabilisD‐amino acid oxidase using poly(ethylene glycol) maleimide and its effect on activity and stability of the enzyme (2007) (17)
α-1 , 4-D-Glucan phosphorylase of gram-positive Corynebacterium callunae : isolation , biochemical properties and molecular shape of the enzyme from solution X-ray scattering (1997) (17)
Structure and mechanism of human UDP-xylose synthase: evidence for a promoting role of sugar ring distortion in a three-step catalytic conversion of UDP-glucuronic acid (2012) (17)
Convenient microtiter plate-based, oxygen-independent activity assays for flavin-dependent oxidoreductases based on different redox dyes (2013) (17)
Regioselective O-glucosylation by sucrose phosphorylase: a promising route for functional diversification of a range of 1,2-propanediols. (2010) (17)
Engineering of Aerococcus viridans L-lactate oxidase for site-specific PEGylation: characterization and selective bioorthogonal modification of a S218C mutant. (2012) (17)
Thermal denaturation pathway of starch phosphorylase from Corynebacterium callunae: Oxyanion binding provides the glue that efficiently stabilizes the dimer structure of the protein (2000) (17)
Stepwise metabolic adaption from pure metabolization to balanced anaerobic growth on xylose explored for recombinant Saccharomyces cerevisiae (2014) (17)
Utilization of xylitol dehydrogenase in a combined microbial/enzymatic process for production of xylitol from d-glucose (2002) (17)
Structure-function relationships for Schizophyllum commune trehalose phosphorylase and their implications for the catalytic mechanism of family GT-4 glycosyltransferases. (2006) (17)
Enzymatic redox isomerization of 1,6-disaccharides by pyranose oxidase and NADH-dependent aldose reductase (2001) (17)
Diastereoselective Synthesis of Glycosyl Phosphates by Using a Phosphorylase–Phosphatase Combination Catalyst (2015) (16)
Fungal trehalose phosphorylase: kinetic mechanism, pH-dependence of the reaction and some structural properties of the enzyme from Schizophyllum commune. (2001) (16)
Exploring the cupin-type metal-coordinating signature of acetylacetone dioxygenase Dke1 with site-directed mutagenesis: catalytic reaction profile and Fe2+ binding stability of Glu-69->Gln mutant (2006) (16)
Galactosyl transfer catalyzed by thermostable beta-glycosidases from Sulfolobus solfataricus and Pyrococcus furiosus: kinetic studies of the reactions of galactosylated enzyme intermediates with a range of nucleophiles. (2001) (16)
α-Retaining glucosyl transfer catalysed by trehalose phosphorylase from Schizophyllum commune: mechanistic evidence obtained from steady-state kinetic studies with substrate analogues and inhibitors (2001) (16)
High-level expression of Rhodotorula gracilisd-amino acid oxidase in Pichia pastoris (2011) (16)
A new maltodextrin-phosphorylase from Corynebacterium callunae for the production of glucose-1-phosphate (1994) (16)
Scale-up and intensification of (S)-1-(2-chlorophenyl)ethanol bioproduction: economic evaluation of whole cell-catalyzed reduction of o-chloroacetophenone. (2013) (16)
Integration of whole-cell reaction and product isolation: Highly hydrophobic solvents promote in situ substrate supply and simplify extractive product isolation. (2017) (15)
Aromatic interactions at the catalytic subsite of sucrose phosphorylase: Their roles in enzymatic glucosyl transfer probed with Phe52 → Ala and Phe52 → Asn mutants (2011) (15)
Utilization of xylitol dehydrogenase in a combined microbial/enzymatic process for production of xylitol from D-glucose. (2002) (15)
Bacterial sialyltransferases and their use in biocatalytic cascades for sialo-oligosaccharide production. (2020) (15)
On the role of Brønsted catalysis in Pseudomonas fluorescens mannitol 2-dehydrogenase. (2003) (15)
Walking a Fine Line with Sucrose Phosphorylase: Efficient Single‐Step Biocatalytic Production of l‐Ascorbic Acid 2‐Glucoside from Sucrose (2017) (15)
Saccharomyces cerevisiae strain comparison in glucose–xylose fermentations on defined substrates and in high-gravity SSCF: convergence in strain performance despite differences in genetic and evolutionary engineering history (2017) (15)
Preparative Asymmetric Synthesis of Canonical and Non‐canonical α‐amino Acids Through Formal Enantioselective Biocatalytic Amination of Carboxylic Acids (2019) (15)
Tunable Semicrystalline Thin Film Cellulose Substrate for High-Resolution, In-Situ AFM Characterization of Enzymatic Cellulose Degradation. (2015) (15)
Structural and functional properties of aldose xylose reductase from the D-xylose-metabolizing yeast Candida tenuis. (2001) (14)
Whole cell-based catalyst for enzymatic production of the osmolyte 2-O-α-glucosylglycerol (2021) (14)
Systematic strain construction and process development: Xylitol production by Saccharomyces cerevisiae expressing Candida tenuis xylose reductase in wild-type or mutant form. (2015) (14)
The Ala95‐to‐Gly substitution in Aerococcus viridans l‐lactate oxidase revisited – structural consequences at the catalytic site and effect on reactivity with O2 and other electron acceptors (2015) (14)
Leloir glycosyltransferases of natural product C-glycosylation: structure, mechanism and specificity. (2020) (14)
Downstream Processing of Nucleoside‐Diphospho‐Sugars from Sucrose Synthase Reaction Mixtures at Decreased Solvent Consumption (2016) (14)
Design of experiments reveals critical parameters for pilot-scale freeze-and-thaw processing of L-lactic dehydrogenase. (2015) (14)
Dissecting differential binding of fructose and phosphate as leaving group/nucleophile of glucosyl transfer catalyzed by sucrose phosphorylase (2007) (14)
Saturation–transfer–difference NMR to characterize substrate binding recognition and catalysis of two broadly specific glycoside hydrolases (2006) (14)
Single‐Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts (2018) (14)
Electrostatic stabilization in a pre-organized polar active site: the catalytic role of Lys-80 in Candida tenuis xylose reductase (AKR2B5) probed by site-directed mutagenesis and functional complementation studies. (2005) (13)
Enzymatic Synthesis of Mannitol: Reaction Engineering for a Recombinant Mannitol Dehydrogenase (1998) (13)
Structure Based Descriptors for the Estimation of Colloidal Interactions and Protein Aggregation Propensities (2013) (13)
A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose (2020) (13)
A Kinase‐Independent One‐Pot Multienzyme Cascade for an Expedient Synthesis of Guanosine 5′‐Diphospho‐d‐mannose (2016) (13)
Mechanistic characterization of UDP‐glucuronic acid 4‐epimerase (2020) (13)
The phosphate site of trehalose phosphorylase from Schizophyllum commune probed by site‐directed mutagenesis and chemical rescue studies (2008) (13)
Crystal structure of Pseudomonas fluorescens mannitol 2-dehydrogenase: evidence for a very divergent long-chain dehydrogenase family. (2003) (13)
Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum. (2019) (13)
Glycosyltransferase Co‐Immobilization for Natural Product Glycosylation: Cascade Biosynthesis of the C ‐Glucoside Nothofagin with Efficient Reuse of Enzymes (2021) (12)
Real-time measurement and modeling of intraparticle pH gradient formation in immobilized cephalosporin C amidase (2013) (12)
Stability and stabilization of D-amino acid oxidase from the yeast Trigonopsis variabilis. (2007) (12)
Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae. Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis. (2003) (12)
Studying non-covalent enzyme carbohydrate interactions by STD NMR. (2008) (12)
d-Xylulose kinase from Saccharomyces cerevisiae: Isolation and characterization of the highly unstable enzyme, recombinantly produced in Escherichia coli (2011) (12)
Design of the Enzyme-Carrier Interface to Overcome the O2 and NADH Mass Transfer Limitations of an Immobilized Flavin Oxidase. (2020) (12)
Catalytic mechanism of Zn2+-dependent polyol dehydrogenases: kinetic comparison of sheep liver sorbitol dehydrogenase with wild-type and Glu154-->Cys forms of yeast xylitol dehydrogenase. (2007) (12)
Isotope Probing of the UDP‐Apiose/UDP‐Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage (2017) (12)
Polyol-specific long-chain dehydrogenases/reductases of mannitol metabolism in Aspergillus fumigatus: biochemical characterization and pH studies of mannitol 2-dehydrogenase and mannitol-1-phosphate 5-dehydrogenase. (2009) (12)
Simultaneous enzymatic synthesis of gluconic acid and sorbitol (1998) (12)
Enzymes of mannitol metabolism in the human pathogenic fungus Aspergillus fumigatus – kinetic properties of mannitol‐1‐phosphate 5‐dehydrogenase and mannitol 2‐dehydrogenase, and their physiological implications (2011) (12)
Leloir glycosyltransferases enabled to flow synthesis: Continuous production of the natural C‐glycoside nothofagin (2021) (12)
Biochemical Characterization and Mechanistic Analysis of the Levoglucosan Kinase from Lipomyces starkeyi (2018) (12)
The oxyanion hole of Pseudomonas fluorescens mannitol 2-dehydrogenase: a novel structural motif for electrostatic stabilization in alcohol dehydrogenase active sites. (2010) (11)
Bacterial alpha-glucan phosphorylases. (1999) (11)
The xylose reductase (AKR2B5) structure: homology and divergence from other aldo-keto reductases and opportunities for protein engineering. (2003) (11)
Model-based analysis of biocatalytic processes and performance of microbioreactors with integrated optical sensors. (2019) (11)
Enzyme Catalytic Promiscuity: The Nonheme Fe2+ Center of β‐Diketone‐Cleaving Dioxygenase Dke1 Promotes Hydrolysis of Activated Esters (2010) (11)
Phosphoryl Transfer from α-d-Glucose 1-Phosphate Catalyzed by Escherichia coli Sugar-Phosphate Phosphatases of Two Protein Superfamily Types (2014) (11)
Transgalactosylation by thermostable beta-glycosidases from Pyrococcus furiosus and Sulfolobus solfataricus. Binding interactions of nucleophiles with the galactosylated enzyme intermediate make major contributions to the formation of new beta-glycosides during lactose conversion. (2000) (11)
Integrated approach for production of recombinant acetylacetone dioxygenase from Acinetobacter johnsonii (2005) (11)
Tailor-made resealable micro(bio)reactors providing easy integration of in situ sensors (2017) (11)
Sequence determinants of nucleotide binding in Sucrose Synthase: improving the affinity of a bacterial Sucrose Synthase for UDP by introducing plant residues (2016) (11)
Entrapment in E. coli improves the operational stability of recombinant beta-glycosidase CelB from Pyrococcus furiosus and facilitates biocatalyst recovery. (2007) (10)
Confocal Luminescence Lifetime Imaging with Variable Scan Velocity and Its Application to Oxygen Sensing. (2016) (10)
Process intensification for cytochrome P450 BM3‐catalyzed oxy‐functionalization of dodecanoic acid (2020) (10)
Chiral resolution through stereoselective transglycosylation by sucrose phosphorylase: application to the synthesis of a new biomimetic compatible solute, (R)-2-O-α-D-glucopyranosyl glyceric acid amide. (2014) (10)
Simultaneous Enzymatic Synthesis of Mannitol and Gluconic Acid: I. Characterization Of The Enzyme System (1996) (10)
Identification of novel metabolic interactions controlling carbon flux from xylose to ethanol in natural and recombinant yeasts (2015) (10)
Enzyme Immobilization by Microencapsulation: Methods, Materials, and Technological Applications (2014) (10)
Toward “homolactic” fermentation of glucose and xylose by engineered Saccharomyces cerevisiae harboring a kinetically efficient l‐lactate dehydrogenase within pdc1‐pdc5 deletion background (2017) (10)
Relationships between structure, function and stability for pyridoxal 5'-phosphate-dependent starch phosphorylase from Corynebacterium callunae as revealed by reversible cofactor dissociation studies. (2004) (10)
Deciphering the enzymatic mechanism of sugar ring contraction in UDP-apiose biosynthesis (2019) (10)
On the donor substrate dependence of group‐transfer reactions by hydrolytic enzymes: Insight from kinetic analysis of sucrose phosphorylase‐catalyzed transglycosylation (2020) (10)
Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+‐dependent oxygenase that cleaves β‐diketones (2009) (10)
Xylose reductase from the Basidiomycete fungus Cryptococcus flavus: purification, steady-state kinetic characterization, and detailed analysis of the substrate binding pocket using structure-activity relationships. (2003) (10)
Stereo-electronic control of reaction selectivity in short-chain dehydrogenases: Decarboxylation, epimerization, and dehydration. (2020) (10)
Hydrolysis of Lactose by ß-Glycosidase CelB from Hyperthermophilic Archaeon Pyrococcus Furiosus (2002) (10)
Maltodextrin Phosphorylase from Escherichia coli: Production and Application for the Synthesis of α‐Glucose‐1‐Phosphate a (1996) (9)
All-in-one assay for β-d-galactoside sialyltransferases: Quantification of productive turnover, error hydrolysis, and site selectivity. (2015) (9)
Mechanism of thermal denaturation of maltodextrin phosphorylase from Escherichia coli. (2000) (9)
Active‐Site His85 of Pasteurella dagmatis Sialyltransferase Facilitates Productive Sialyl Transfer and So Prevents Futile Hydrolysis of CMP‐Neu5Ac (2017) (9)
Efficient downstream processing of maltodextrin phosphorylase from Escherichia coli and stabilization of the enzyme by immobilization onto hydroxyapatite. (1997) (9)
Efficient production of mannan-degrading enzymes by the basidiomycete Sclerotium rolfsii. (1998) (9)
Removal of glycerol from enzymatically produced 2-α-d-glucosyl-glycerol by discontinuous diafiltration (2020) (9)
Synthesis of 5,6-dimodified open-chain d-fructose derivatives and their properties as substrates of bacterial polyol dehydrogenase (2000) (9)
Non-native aggregation of recombinant human granulocyte-colony stimulating factor under simulated process stress conditions. (2012) (9)
Simultaneous Enzymatic Synthesis of Gluconic Acid and Sorbitol (1997) (9)
Speeding up the product release: a second‐sphere contribution from Tyr191 to the reactivity of l‐lactate oxidase revealed in crystallographic and kinetic studies of site‐directed variants (2015) (9)
Fungal trehalose phosphorylase: kinetic mechanism, pH-dependence of the reaction and some structural properties of the enzyme from Schizophyllum commune (2001) (8)
Substrate-binding recognition and specificity of trehalose phosphorylase from Schizophyllum commune examined in steady-state kinetic studies with deoxy and deoxyfluoro substrate analogues and inhibitors. (2002) (8)
NMR study of 13C-kinetic isotope effects at 13C natural abundance to characterize oxidations and an enzyme-catalyzed reduction (2006) (8)
β-Glucosyl Fluoride as Reverse Reaction Donor Substrate and Mechanistic Probe of Inverting Sugar Nucleotide-Dependent Glycosyltransferases (2018) (8)
Purification and characterization of a laccase from the white-rot fungus Trametes multicolor (2002) (8)
Combining expression and process engineering for high-quality production of human sialyltransferase in Pichia pastoris. (2016) (8)
Harnessing Candida tenuis and Pichia stipitis in whole‐cell bioreductions of o‐chloroacetophenone: Stereoselectivity, cell activity, in situ substrate supply and product removal (2013) (8)
Substitution of the catalytic acid–base Glu237 by Gln suppresses hydrolysis during glucosylation of phenolic acceptors catalyzed by Leuconostoc mesenteroides sucrose phosphorylase (2010) (8)
A Parsimonious Mechanism of Sugar Dehydration by Human GDP-Mannose-4,6-dehydratase (2019) (8)
Yihx-encoded haloacid dehalogenase-like phosphatase HAD4 from Escherichia coli is a specific α-d-glucose 1-phosphate hydrolase useful for substrate-selective sugar phosphate transformations (2014) (8)
Combining a Genetically Engineered Oxidase with Hydrogen‐Bonded Organic Frameworks (HOFs) for Highly Efficient Biocomposites (2021) (8)
High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities (2014) (8)
Plasmid Design for Tunable Two‐Enzyme Co‐Expression Promotes Whole‐Cell Production of Cellobiose (2020) (8)
CorNet: Assigning function to networks of co-evolving residues by automated literature mining (2017) (8)
Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild‐type and His334→Gly mutant enzymes (2007) (7)
Examining the role of phosphate in glycosyl transfer reactions of Cellulomonas uda cellobiose phosphorylase using D-glucal as donor substrate. (2012) (7)
Tunable mixed amorphous–crystalline cellulose substrates (MACS) for dynamic degradation studies by atomic force microscopy in liquid environments (2014) (7)
An ortho C-methylation/O-glycosylation motif on a hydroxy-coumarin scaffold, selectively installed by biocatalysis. (2017) (7)
Orthophosphate binding at the dimer interface of Corynebacterium callunae starch phosphorylase: mutational analysis of its role for activity and stability of the enzyme (2010) (7)
Enzymatic Regeneration of NAD in Enantioselective Oxidation of Secondary Alcohols: Glutamate Dehydrogenase Versus NADH Dehydrogenase (1998) (7)
Acceleration of an aldo-keto reductase by minimal loop engineering (2014) (7)
Tyr‐51 is the proton donor–acceptor for NAD(H)‐dependent interconversion of xylose and xylitol by Candida tenuis xylose reductase (AKR2B5) (2008) (7)
Reactive extraction of fructose for efficient separation of sucrose-derived glucosides produced by enzymatic glycosylation (2020) (7)
Enzymatic Synthesis of α-Glucose-1-phosphate: A Study Employing a New α-1,4 Glucan Phosphorylase from Corynebacterium callunae 1 (1995) (6)
Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material. (2021) (6)
Altering dimer contacts in xylose reductase from Candida tenuis by site-directed mutagenesis: structural and functional properties of R180A mutant. (2003) (6)
Immobilization of CMP‐Sialic Acid Synthetase and α2,3‐Sialyltransferase for Cascade Synthesis of 3′‐Sialyl β‐D‐Galactoside with Enzyme Reuse (2022) (6)
Dynamic Mechanism of Proton Transfer in Mannitol 2-Dehydrogenase from Pseudomonas fluorescens (2011) (6)
Understanding the silica-based sol-gel encapsulation mechanism of Thermomyces lanuginosus lipase: The role of polyethylenimine (2018) (6)
Mutagenesis of the dimer interface region of Corynebacterium callunae starch phosphorylase perturbs the phosphate-dependent conformational relay that enhances oligomeric stability of the enzyme. (2003) (6)
Control of the association state of tetrameric glucose-fructose oxidoreductase from Zymomonas mobilis as the rationale for stabilization of the enzyme in biochemical reactors. (1998) (6)
Modeling the activity burst in the initial phase of cellulose hydrolysis by the processive cellobiohydrolase Cel7A (2019) (6)
Binding energy and specificity in the catalytic mechanism of yeast aldose reductases (1999) (6)
Direct-Write Fabrication of Cellulose Nano-Structures via Focused Electron Beam Induced Nanosynthesis (2016) (6)
Continuous process technology for glucoside production from sucrose using a whole cell-derived solid catalyst of sucrose phosphorylase (2021) (6)
Strategies to an Efficient Enzymatic Production of Xylitol (1998) (6)
Binding energy and specificity in the catalytic mechanism of yeast aldose reductases. (1999) (6)
The role of Cys108 in Trigonopsis variabilis d-amino acid oxidase examined through chemical oxidation studies and point mutations C108S and C108D. (2010) (6)
The catalytic mechanism of NADH-dependent reduction of 9,10-phenanthrenequinone by Candida tenuis xylose reductase reveals plasticity in an aldo-keto reductase active site. (2009) (6)
Binding and catalysis by yeast aldose reductase: a substrate-analog approach with new aldose derivatives. (1999) (5)
Reverse C-glycosidase reaction provides C-nucleotide building blocks of xenobiotic nucleic acids (2020) (5)
Two N-terminally truncated variants of human β-galactoside α2,6 sialyltransferase I with distinct properties for in vitro protein glycosylation. (2016) (5)
Study of the thermal stability of D-amino acid oxidase from Trigonopsis variabilis reveals enzyme inactivation via multiple steps (2006) (5)
Preparation of 2-O-glyceryl-a-D-glucopyranoside from a glucosyl donor and a glucosyl acceptor (2008) (5)
Kinetic Analysis and Probing with Substrate Analogues of the Reaction Pathway of the Nitrile Reductase QueF from Escherichia coli* (2016) (5)
Comparison of broad-scope assays of nucleotide sugar-dependent glycosyltransferases. (2015) (5)
From Alcohol Dehydrogenase to a “One-way” Carbonyl Reductase by Active-site Redesign (2010) (5)
Probing enzyme–substrate interactions at the catalytic subsite of Leuconostoc mesenteroides sucrose phosphorylase with site-directed mutagenesis: the roles of Asp49 and Arg395 (2012) (5)
Catalytic mechanism of human UDP-glucose 6-dehydrogenase: in situ proton NMR studies reveal that the C-5 hydrogen of UDP-glucose is not exchanged with bulk water during the enzymatic reaction (2012) (5)
Expanding the Enzyme Repertoire for Sugar Nucleotide Epimerization: the CDP-Tyvelose 2-Epimerase from Thermodesulfatator atlanticus for Glucose/Mannose Interconversion (2020) (4)
Efficient enzyme formulation promotes Leloir glycosyltransferases for glycoside synthesis. (2020) (4)
Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides (2021) (4)
Candida tenuis xylose reductase catalyzed reduction of aryl ketones for enantioselective synthesis of active oxetine derivatives. (2012) (4)
Glycosides as Compatible Solutes: Biosynthesis and Applications (2011) (4)
Enzyme Immobilization in Wall-Coated Flow Microreactors. (2020) (4)
Crystallographic snapshots of UDP-glucuronic acid 4-epimerase ligand binding, rotation, and reduction (2020) (4)
Precision synthesis of reducing-end thiol-modified cellulose enabled by enzyme selection (2021) (4)
Continuous production of α,α-trehalose by immobilised fungal trehalose phosphorylase (1999) (4)
Enzyme Synergy in Transient Clusters of Endo- and Exocellulase Enables a Multilayer Mode of Processive Depolymerization of Cellulose (2022) (4)
Engineering analysis of multienzyme cascade reactions for 3ʹ‐sialyllactose synthesis (2021) (3)
Ionic liquid as dual-function catalyst and solvent for efficient synthesis of sucrose fatty acid esters (2022) (3)
Pushing the limits: Cyclodextrin-based intensification of bioreductions. (2020) (3)
Oxyanion-Mediated Protein Stabilization: Differential Roles of Phosphate for Preventing Inactivation of Bacterial α-Glucan Phosphorylases (2001) (3)
Oscillating flow bioreactors: An enabling technology for sustainable biorefining operations? (2020) (3)
Separation behavior and microstructure of emulsified, two-phasic E. coli bioreaction mixtures (2020) (3)
Binding pattern of intermediate UDP-4-keto-xylose to human UDP-xylose synthase: Synthesis and STD NMR of model keto-saccharides. (2017) (3)
Stability and stabilization of α-1,4-d-glucan phosphorylases (1998) (3)
Selective β-Mono-Glycosylation of a C15-Hydroxylated Metabolite of the Agricultural Herbicide Cinmethylin Using Leloir Glycosyltransferases (2021) (3)
Continuous‐Flow Microchannel Reactors with Surface‐Immobilized Biocatalysts (2009) (3)
Hydrophilic Nonwoven Nanofiber Membranes as Nanostructured Supports for Enzyme Immobilization (2022) (3)
Production of Hemicellulose- and Cellulose-Degrading enzymes by various strains ofSclerotium Rolfsii (1997) (3)
Hydrolysis of lactose by β-glycosidase CelB from hyperthermophilic archaeon Pyrococcus furiosus (2002) (3)
Reaction engineering aspects of alpha-l,4-D-glucan phosphorylase catalysis : comparison of plant and bacterial enzymes for the continuous synthesis of D-glucose-1-phosphate. (1997) (3)
Processive Enzymes Kept on a Leash: How Cellulase Activity in Multienzyme Complexes Directs Nanoscale Deconstruction of Cellulose (2021) (3)
Biocatalytic Production of 2-α-d-Glucosyl-glycerol for Functional Ingredient Use: Integrated Process Design and Techno-Economic Assessment (2022) (3)
Alpha-retaining glucosyl transfer catalysed by trehalose phosphorylase from Schizophyllum commune: mechanistic evidence obtained from steady-state kinetic studies with substrate analogues and inhibitors. (2001) (3)
Multi-enzymatic synthesis of UDP-xylose (2014) (2)
Inside Cover: Oriented Immobilization of Enzymes Made Fit for Applied Biocatalysis: Non‐Covalent Attachment to Anionic Supports using Zbasic2 Module (ChemCatChem 8/2011) (2011) (2)
Essential Functional Interplay of the Catalytic Groups in Acid Phosphatase (2022) (2)
Nano-fibrillated cellulose-based scaffolds for enzyme (co)-immobilization: Application to natural product glycosylation by Leloir glycosyltransferases. (2022) (2)
Oscillatory Flow Bioreactor (OFB) Applied in Enzymatic Hydrolysis at High Solid Loadings (2020) (2)
Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli (2018) (2)
Reductive enzymatic dynamic kinetic resolution affording 115 g/L (S)-2-phenylpropanol (2021) (2)
THE STRUCTURE OF XYLOSE REDUCTASE, A DIMERIC ALDO-KETO REDUCTASE FROM CANDIDA TENUIS (2002) (2)
Trehalose phosphorylase from Pleurotus ostreatus: characterization and stabilization by covalent modification, and application for the synthesis of alpha,alpha-trehalose. (2007) (2)
Biocatalytic cascade transformations for the synthesis of C-nucleosides and N-nucleoside analogs. (2023) (2)
Adsorption and desorption of self-assembled L-cysteine monolayers on nanoporous gold monitored by in situ resistometry (2019) (2)
Pyranose oxidase for the production of carbohydrate-based food ingredients (2000) (2)
Interplay of catalytic subsite residues in the positioning of α-d-glucose 1-phosphate in sucrose phosphorylase (2015) (2)
Identification of disulfide-crosslinks in protein aggregates (2011) (1)
Starch phosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. (2007) (1)
Special issue: enzyme technology and biocatalysis. (2007) (1)
N-terminally truncated glycosyltransferases for use in post-translational sialylation (2015) (1)
Nanoporous gold electrodes modified with self-assembled monolayers for electrochemical control of the surface charge. (2021) (1)
Unexpected NADPH Hydratase Activity in the Nitrile Reductase QueF from Escherichia coli (2019) (1)
Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli (2019) (1)
Characterization of a multifunctional alpha2,3-sialyltransferase from Pasteurella dagmatis, carrying a natural Ser-to-Thr substitution (2013) (1)
Production of a novel pyranose 2-0xidase by basidiomyceteTrametes multicolor (1998) (1)
Structural and biochemical characterization of a monolignol oxidoreductase from A. thaliana implies a link between the berberine bridge enzyme-like family and cell wall metabolism (2015) (1)
Microstructured Immobilized Enzyme Reactors for Biocatalysis (2010) (1)
On the donor substrate dependence of group transfer reactions by hydrolytic enzymes: insight from kinetic analysis of sucrose phosphorylase-catalyzed transglycosylation (2020) (1)
Candida tenuis Xylose reductase (AKR2B5) utilizes different catalytic mechanisms for NADH-dependent carbonyl group reduction in aldehyde and quinone substrates (2008) (1)
CREATING A WATER-SOLUBLE RESVERATROL-BASED ANTIOXIDANT THROUGH SITE-SELECTIVE ENZYMATIC GLUCOSYLATION (2016) (1)
On the Role of a Balanced Cofactor Recycling between Xylose Reductase and Xylitol Dehydrogenase in Recombinant Xylose Fermenting Strains of Saccharomyces cerevisiae (2010) (1)
A catalytic consensus motif for long-chain mannitol dehydrogenases revealed by site-directed mutagenesis of the enzyme from Pseudomonas fluorescens (2002) (1)
Monitoring and control of the release of soluble O2 from H2O2 inside porous enzyme carrier for O2 supply to an immobilized d‐amino acid oxidase (2022) (1)
Process Stability of Glucose‐Fructose Oxidoreductase from Zymomonas mobilis: Role of Reactive Thiols Probed by Chemical Modification (1998) (1)
Pseudomonas fluorescens mannitol 2-dehydrogenase ternary complex with NAD and D-mannitol (2002) (1)
Probing of the reaction pathway of human UDP-xylose synthase with site-directed mutagenesis. (2015) (1)
P450Jα - A new, robust and α-selective fatty acid hydroxylase displaying unexpected 1-alkene formation. (2020) (1)
Stability and stabilization of glucose-fructose oxidoreductase from Zymomonas mobilis against irreversible inactivation during substrate turnover in biochemical reactors (1998) (1)
Role of Phe-114 in substrate specificity of Candida tenuis xylose reductase (AKR2B5) (2007) (1)
Fusion to a Pull‐Down Module (2014) (1)
Rapid preparation in high yield of pure formate dehydrogenase from Pichia angusta (1998) (1)
Saturation transfer difference NMR to study substrate and product binding to human UDP-xylose synthase (hUXS1A) during catalytic event (2015) (1)
Efficient production of mannan-degrading enzymes by the basidiomyceteSclerotium rolfsii (1998) (1)
Engineering cascade biocatalysis in whole cells for bottom-up synthesis of cello-oligosaccharides: flux control over three enzymatic steps enables soluble production (2022) (1)
Reaction Engineering Aspects of α-l,4-D-Glucan Phosphorylase Catalysis (1997) (1)
3D Printed Porous Nanocellulose-Based Scaffolds As Carriers for Immobilization of Glycosyltransferases (2022) (1)
Rec. ST6Gal-I variants to control enzymatic activity in processes of in vitro glycoengineering (2013) (1)
Carbohydrate-active enzymes: components of mechanism and applications in biotechnology (2009) (0)
Modeling cellulase-cellulose adsorption and the kinetic of the enzymatic hydrolysis of microcrystalline cellulose (1993) (0)
Structure-function relationship for Schizophyllum commune trehalose phosphorylase probed by site-directed mutagenesis and kinetic studies (2007) (0)
Freezing and Thawing of Protein Solutions – Introducing a Lab-Scale Freezing Container (2011) (0)
FUCOSYLTRANSFERASES: KEY PLAYERS IN THE BIOECHNOLOGICAL SYNTHESIS OF HUMAN MILK OLIGOSACCHARIDES (2015) (0)
Effect of ionic liquid on the enzymatic synthesis of cello-oligosaccharides and their assembly into cellulose materials. (2022) (0)
Fabrication of Cellulose Structures via Focused Electron Beam Induced Conversion: Approaching the Nanoscale (2016) (0)
Aerococcus viridans L-lactate oxidase Y191F mutant (2015) (0)
Biochemical properties and structure-function relationships for recombinant fungal trehalose phosphorylase from Schizophyllum commune (2005) (0)
Continuous process technology for bottom-up synthesis of soluble cello-oligosaccharides by immobilized cells co-expressing three saccharide phosphorylases (2022) (0)
Enzymatic Cellulose Hydrolysis: New Approaches in the Investigation of Biocatalytic Processes via In-situ Liquid Atomic Force Microscopy (2013) (0)
Apo-structure of alpha2,3-sialyltransferase variant 2 from Pasteurella dagmatis (2015) (0)
Critical evaluation of different NDP-sugar purification strategies (2015) (0)
Steps towards a whole cell bioreduction system –Reduction of ethyl benzoylformate (2006) (0)
Researchengineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst (2010) (0)
Enzyme technology and biocatalysis (2007) (0)
alpha-retaining glucosyl transfer catalyzed by starch phosphorylase from Corynebacterium callunae: The role of active-site histidine-334 (2005) (0)
CARB 4-Elucidating the mechanism of human UDP-glucose dehydrogenase using kinetic, mutational and structural studies (2008) (0)
Mechanism and application of bacterial sialyltransferase (2014) (0)
A newly discovered multifunctional α2,3-sialyltransferase from Pasteurella dagmatis carries a natural Ser-to-Thr substitution (2013) (0)
Crystal structure of the Lys-274 to Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NADP+ (2005) (0)
A novel multifunctional sialyltransferase PdST from Pasteurella dagmatis (2012) (0)
Aerococcus viridans L-lactate oxidase mutant (2014) (0)
An oxygen-independent activity assay for Trigonopsis variabilis D-amino acid oxidase and the characterization of a technical grade soluble enzyme towards thermal stability (2004) (0)
Enzymatic production of 2-O-(alpha-D-glucopyranosyl)-glycerate using sucrose phosphorylase (2012) (0)
Sustainability assessment integrated in bioprocess design in H2020 Project CARBAFIN: When thinking about process optimization - are we also thinking about environmental aspects? (2019) (0)
Crystal structure of human GDP-D-mannose 4,6-dehydratase in complex with GDP-4k6d-Man (2018) (0)
Investigating Freeze and Thaw Behavior of Protein Solutions using a Lab-Scale Freezing Container (2012) (0)
Sucrose Phosphorylase in Carbohydrate Synthesis - Mechanistic and Synthetic Considerations (2010) (0)
The role of aromatic interactions in the catalytic mechanism of sucrose phosphorylase (2011) (0)
Non-heme Fe(II)-dependent diketone dioxygenase Dke1: The impact of the first and second coordination sphere of the metal-center on cofactor stability and catalysis (2005) (0)
Stabilization by targeting biological activity into inclusion bodies (2009) (0)
Structure-based approach of harnessing aldo-keto reductases for biocatalysis. (2009) (0)
from Pasteurella dagmatis (2013) (0)
Solubility Enhancement by Site-Selective Polyphenol Glucosylation (2016) (0)
Protein engineering approach to alter chemoselectivity, (co-)substrate specificity and enantioselectivity of selected reductases (2008) (0)
Examining the inactivation of Trigonopsis variabilisD-amino acid oxidase using different probes of protein integrity and conformational stability (2004) (0)
Steps towards the rational design of an immobilized biocatalyst with improved process stability (2007) (0)
Structure and mechanism of oxidoreductases delivering precursors to human glycosaminoglycan and proteoglycan biosynthesis (2011) (0)
Editorial Board: Biotechnology Journal 10/2017 (2017) (0)
Dissecting the stabilising effects of immobilisation in D-amino acid oxidase (2007) (0)
The mechanism of inactivation as rationale for stabilization of glucose-fructose oxidoreductase in biochemical reactors. (1998) (0)
Structure-function relationships for xylose reductase from the yeast Candida tenuis. (2002) (0)
The Cupin Fold Signature: Conserved Residue Glu69 of the Second Coordination Sphere of the Fe2+ Cofactor and Its Role for Stability and Catalysis in Dke1 (2005) (0)
Structure and function of human UDP-glucose dehydrogenase (2010) (0)
Pharmaceutical use of nanocellulose produced by enzymes (2020) (0)
Limitations in Xylose Fermenting Strains of Saccharomyces cerevisiae: Is Coenzyme Recycling between Xylose Reductase and Xylitol Dehydrogenase Sufficient to Prevent Xylitol Formation? (2010) (0)
Crystal structure of human UDP-glucose dehydrogenase Glu161Gln, in complex with thiohemiacetal intermediate (2009) (0)
Catalytic mechanism and specificity of yeast aldose reductase: structure/reactivity studies and role of noncovalent enzyme/aldehyde interactions (2000) (0)
Crystal structure of H114A mutant of Candida tenuis xylose reductase (2004) (0)
Oxidative biotransformations: stabilising effects of immobilization in D-amino acid oxidase (2006) (0)
Utilization of lactose: I. Efficient enzymatic production of lactobionic acid (2000) (0)
Action of an AA9 - type lytic polysaccharide monooxygenase on the cellulose surface and its effect on cellulase hydrolytic efficiency (2015) (0)
UDP-glucuronic acid decarboxylase: structure, catalytic mechanism, and application (2010) (0)
Crystal structure of mannitol dehydrogenase in complex with NAD (2002) (0)
Editorial: Biocatalytic opportunities to harness the structural diversity of carbohydrates. (2021) (0)
Aggregation of hGH - Insights by accelerated stress studies and reversible refolding (2012) (0)
Enzymatic redox cascade for synthesis of nucleotide sugars (2014) (0)
Cover Image, Volume 113, Number 9, September 2016. (2016) (0)
Daniel et al_2015_revised (2015) (0)
Characterisation of oxidative modification of D-amino acid oxidase by electrospray ionization mass spectroscopy (2005) (0)
How does broadly specific Candida tenuis xylose reductase (CtXR) bring about the requisite selectivity for its natural substrate D-xylose? (2006) (0)
Oxyanion linkers provide a most efficient glue in the stabilization of the dimer structure of starch phosphorylase from Corynebacterium callunae (2009) (0)
Reaction intensification for biocatalytic production of polyphenolic natural product di-C-β-glucosides. (2023) (0)
Cooperative action of Trichoderma reesei cellulases during cellulose degradation (1994) (0)
Three‐level hybrid modeling for systematic optimization of biocatalytic synthesis: α‐glucosyl glycerol production by enzymatic trans‐glycosylation from sucrose (2021) (0)
What do we need the enzyme for? The role of the enzyme environment for the chemical mechanism of the non-heme Fe(II)-dependent diketone dioxygenase Dke1 (2005) (0)
Elucidating the key amino acids for the unusual formation of C-C bonds by a glycosyltransferase from Oryza sativa (2010) (0)
Evaluation methodology and multi-level engineering approach to improve whole cell ketone reduction (2009) (0)
Saturation Transfer Difference (STD) NMR to characterise binding of substrates, intermediates, and products to biocatalysts (2005) (0)
Freezing Process Development under QbD Principles - In-silico Solutions for Process Characterization and Scaling (2013) (0)
On the roles of auxiliary proteins and enzymes in cellulose surface degradation: swollenin and lytic polysaccharide monooxygenase (2016) (0)
Stirred, not shaken –process-relevant parameters and their effects on stability of human growth hormone (2010) (0)
Some factors limiting rate and yield in the hydrolysis of lignocellulose (1996) (0)
Cys108 is involved in maintenance of D-amino acid oxidase active site environment: a comparison of wild-type enzyme with oxidized, fragmented and mutant variants (2006) (0)
The structure of alpha2,3-sialyltransferase variant 1 from Pasteurella dagmatis in complex with the donor product CMP (2015) (0)
Engineering the Cofactor Preference of Xylose Reductase and Xylitol Dehydrogenase to Enhance Xylose Fermentation by Recombinant Saccharomyces cerevisiae (2009) (0)
Oxygenases of the cupin-superfamily - insights into the mechanism of the non-heme iron dependent dioxygenase Dke1 (2004) (0)
Novel High Throughput Screening Methodologies to Advance Directed Evolution of Glycosyltransferases (2011) (0)
Integration of enzyme, strain and reaction engineering – Production of alpha-hydroxy esters (2007) (0)
Crystal Structure Of An Engineered K274RN276D Double Mutant of Xylose Reductase From Candida Tenuis Optimized To Utilize NAD (2004) (0)
Visualizing cellulase activity on the cellulose surface (2014) (0)
Insight into C-glycosidic Bond Formation by Plant Glycosyl Transferases (2012) (0)
Model of Processive Catalysis with Site Clustering and Blocking and Its Application to Cellulose Hydrolysis (2022) (0)
Biochemical Characterization of Mannitol-1-phosphate 5-Dehydrogenase and Mannitol 2-Dehydrogenase from the Human Pathogen Aspergillus fumigatus (2008) (0)
Back Cover: Targeting the Substrate Binding Site of E. coli Nitrile Reductase QueF by Modeling, Substrate and Enzyme Engineering (Chem. Eur. J. 22/2013) (2013) (0)
Exploring the catalytic mechanism of sucrose phosphorylase with site-directed mutagenesis (2006) (0)
Expression of human sialyltransferase in yeast (2015) (0)
Mechanism of sucrose phosphorylase from Leuconostoc mesenteroides, a member of glycosidase family GH-13 (2006) (0)
Exploiting the biocatalytic potential of glycosyltransferases through in situ sugar donor recycling (2016) (0)
Correction: Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the β-N-hexosaminidase from Bifidobacterium bifidum. (2019) (0)
Relationships between Structure and Specificity for Candida tenuis Xylose Reductase (AKR2B5) (2004) (0)
Enzymatic redox cascade for synthesis of sugar nucleotides (2014) (0)
Synthesis of sorbitol and gluconic acid by glucose-fructose oxidoreductase from Zymomonas mobilis in continuous membrane reactors (1995) (0)
Crystal structure of human GDP-D-mannose 4,6-dehydratase (E157Q) in complex with GDP-Man (2018) (0)
Design of Novel Enzyme Functionalities: Biochemical and biocatalytic characterization (2011) (0)
Stability of L-Lactic Dehydrogenase in a Lab-Scale Freezing Container (2012) (0)
Crystal structure of human GDP-D-mannose 4,6-dehydratase (S156D) in complex with GDP-Man (2019) (0)
Electrostatic stabilization in alcohol dehydrogenase mechanisms: the oxyanion hole of D-mannitol 2-dehydrogenase from Pseudomonas fluorescens is a novel structural motif that facilitates the catalytic step of hydride transfer (2006) (0)
Non-Native Aggregation of rhG-CSF under Simulated Process Conditions (2011) (0)
1.8 Angstrom structure of xylose reductase from Candida tenuis in complex with NAD (2003) (0)
Investigation of Enzyme-Substrate Interactions in Candida tenuis Xylose Reductase (2004) (0)
Enzymatic Dynamic Reductive Kinetic Resolution Towards 115 g/L (S)-2-Phenylpropanol (2021) (0)
Expression of mammalian glycosyltransferases in yeast (2012) (0)
Synergism of Trichoderma reesei cellulases during cellulose degradation. In "Enzymatic Degradation of Insoluble Polysaccharides", (1995) (0)
Aggregation of Recombinant Human Granulocyte-Colony Stimulating Factor under Process Conditions (2011) (0)
Mechanism and specificity of Candida tenuis xylose reductase (AKR2B5). (2006) (0)
Mechanistic characterization and comparison of two anomeric configuration-retaining disaccharide phosphorylases. (2006) (0)
Improving the Water Solubility of Polyphenols through Site-Selective Enzymatic Glucosylation (2016) (0)
Disacc haride Phosphorylases: Mechanistic Diversity and App lication in the Glycosciences* (2011) (0)
Enzymatic C4‐Epimerization of UDP‐Glucuronic Acid: Precisely Steered Rotation of a Transient 4‐Keto Intermediate for an Inverted Reaction without Decarboxylation (2022) (0)
Production of a hypothetical sialyltransferase from P. dagmatis: Cloning, expression, purification and setup of a screening assay (2011) (0)
Flavonoid glucosylation by glycosyltransferase-catalyzed cascade reactions (2014) (0)
Binding and Catalysis by Yeast Aldose Reductase: A Substrate‐Analogue Approach with New Aldose Derivatives. (1999) (0)
THE CETUS PROCESS REVISITED: A NOVEL ENZYMATIC ALTERNATIVE FOR THE D-FRUCTOSE PRODUCTION OF ALDOSE-FREE (1998) (0)
Microfluidic immobilised enzyme reactors for biocatalysis (2008) (0)
Production of mannanases by the phytopathogenic fungus Sclerotium (athelia) rolfsii. (1999) (0)
Function of Pyridoxal-5’-Phosphate in Bacterial Phosphorylases (2000) (0)
Switching between O- and C-glycosylation by active-site motif exchange in plant glycosyltransferases (2013) (0)
The Visualization of Enzymatic Cellulose Degradation Using High Speed Atomic Force Microscopy (2015) (0)
Catalytic mechanisms and synthetic applications of retaining disaccharide phosphorylases (2008) (0)
Push and pull concepts for biocatalytic synthesis (2006) (0)
Changing the coenzyme specificity of xylose reductase from Candida tenuis by site-directed mutagenesis (2003) (0)
DoE-Based Screening for Critical Process Parameters of Freezing and Thawing Proteins in a Pilot Freeze Container (2013) (0)
Glyco-Biotechnology. Oxidoreductases. (2003) (0)
From NMR to enzymatic information (2013) (0)
Aromatic interactions at the catalytic subsite of sucrose phosphorylase: Analysis of their role in enzymatic glucosyl transfer (2011) (0)
Bioinorganic chemistry of a novel non-heme iron dioxygenase -structure activity relationships give insights into the chemical mechanism of enzyme action in Dke1 (2003) (0)
Editorial Board: Biotechnology Journal 1/2018 (2018) (0)
Assessment of protein damage through freeze-thaw effects (2011) (0)
Multiple factors determine the stability of D-amino acid oxidase from Trigonopsis variabilis (2006) (0)
Nitrile Reduction using Nitrile Reductase (2011) (0)
Aerococcus viridans L-lactate oxidase Y215F mutant (2016) (0)
WITHDRAWN: Efficient enzyme formulation promotes Leloir glycosyltransferases for glycoside synthesis. (2020) (0)
Role of enzyme immobilization in the formulation of enzymes for single use (2017) (0)
A selective and atom-economic rearrangement of uridine by cascade biocatalysis for production of pseudouridine (2023) (0)
Glycosyltransferases: On the way towards industrial biocatalysts (2014) (0)
Biocatalytic nitrile reduction with wild type and mutant nitrile reductases (2012) (0)
Retaining glucosyl transfer catalyzed by disaccharide phosphorylases: mechanisms and application in synthesis (2009) (0)
Utilization of lactose: II. Efficient enzymatic production of D-tagatose (2000) (0)
Investigations of the substrate scope of nitrile reductase queF (2012) (0)
Synergistic Effects Among Cellulases during Enzymatic Cellulose Degradation, Visualized by In situ Atomic Force Microscopy (2013) (0)
AFM based Studies into Enzymatic Cellulose Degradation using a novel Substrate (2013) (0)
Crystal structure of human GDP-D-mannose 4,6-dehydratase in complex with GDP-4F-Man (2018) (0)
Multi-level engineering approach to improve ketone reduction by baker's yeast (2008) (0)
Sweet temptation. From structure-function relationships of disaccharide phosphorylases to application in synthesis (2009) (0)
Tunable close-to-nature cellulose for enzymatic degradation studies via dynamic high resolution AFM (2014) (0)
Development chain of bioreductions: Catalyst design, reaction engineering, operation mode, decision guidance (2013) (0)
Characterization of trehalose phosphorylase from Pleurotus ostreatus and stabilisation of the enzyme by covalent modification (2004) (0)
No Job Name (0)
Editorial Board: Biotechnology Journal 2/2019 (2019) (0)
Crystal structure of dimeric xylose reductase in complex with NADP(H) (2002) (0)
Cellulose hydrolysis, probable configuration and operation conditions (1995) (0)
Structure-function relationships for trehalose phosphorylase from Schizophyllum commune (2006) (0)
Debottlenecking enzymatic production of fucosylated HMOs with prokaryotic a1,2-fucosyltransferases (2017) (0)
Intraparticle pH Sensing Within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Particles. (2020) (0)
Exploring the Active Binding Site of Nitrile Reductases (2013) (0)
Synthesis of 5,6‐Dimodified Open‐Chain D‐Fructose Derivatives and Their Properties as Substrates of Bacterial Polyol Dehydrogenase. (2000) (0)
Kinetic Pathway Analysis of Aggregation of Therapeutic Proteins (2010) (0)
Structural and functional characterization of recombinant fungal trehalose phosphorylase from Schizophyllum commune (2004) (0)
Apo-structure of alpha2,3 sialyltransferase from Pasteurella dagmatis (2015) (0)
Enzymes and sugars: Challenges and opportunities for biocatalytic process development (2017) (0)
Chemoselectivity of Candida tenuis xylose reductase - Reduction of Phenylglyoxal (2007) (0)
2-O-(alpha-D-glucopyranosyl)-sn-glycerol I) Development of an efficient biocatalytic production process (2009) (0)
Structure and mechanism of oxidoreductases providing precursors to human glycosaminoglycan biosynthesis: UDP-glucose dehydrogenase and UDP-xylose synthase (2011) (0)
Influence and comparison of accelerated stress conditions on human growth hormone (2011) (0)
On the role of concentration gradients in the performance of carrier-bound immobilized biocatalysts: the case of an industrial amidase (2008) (0)
Preparation of nano-flat cellulose substrates for dynamic degradation experiments via AFM (2011) (0)
Artificial Substrates as Key Element towards Single Enzyme Tracking via High Speed Atomic Force Microscopy in Enzymatic Degradation of Cellulose (2015) (0)
Pyranose oxidase from white-rot fungus Trametes multicolor. (1999) (0)
Hydride Transfer Mechanism of Enzymatic Sugar Nucleotide C2 Epimerization Probed with a Loose-Fit CDP-Glucose Substrate (2022) (0)
Verfahren zur Herstellung von Glucosederivaten / Method for producing glucose derivatives (2008) (0)
Enzymatic Nitrile Reduction (2012) (0)
Glycosyltransferase Cascades Made Fit For the Biocatalytic Production of Natural Product Glycosides (2021) (0)
A determinant of cosubstrate specificity in aldo-keto reductases. (2006) (0)
Controllable Iterative β-Glucosylation from UDP-Glucose by Bacillus cereus Glycosyltransferase GT1: Application for the Synthesis of Disaccharide-Modified Xenobiotics (2021) (0)
Single-molecule study of oxidative enzymatic deconstruction of cellulose (2017) (0)
Visualization in Biotechnology (2011) (0)
Biological nitrile reduction – exploring a remarkable reaction (2013) (0)
Enzymatic method for procudir alpha-d-glucosylglycerol (2-0 glyceryl alpha-d-glucopyranoside). (2007) (0)
Optimal parameters in variable‐velocity scanning luminescence lifetime microscopy (2020) (0)
Synthesis, transport and function of glucosylglycerol in Stenotrophomonas (2007) (0)
Sucrose synthase: a unique glycosyltransferase enroute to biocatalytic process development (2015) (0)
Enzymatic Nitrile Reduction using wild type and mutant Nitrile Reductase QueF (2011) (0)
Structural, kinetic and mutational insights along the reaction coordinate of human UDP-glucose dehydrogenase (2010) (0)
Special Issue on acib, dedicated to the occasion of Prof. Dr. Helmut Schwab's 65th birthday. (2016) (0)
The structure of alpha2,3-sialyltransferase variant 2 from Pasteurella dagmatis in complex with the donor product CMP (2015) (0)
Characterization and biosynthetic application of human UDP-xylose synthase (2012) (0)
Action of an AA9-type lytic polysaccharide monooxygenase on the cellulose surface and its role for synergy with cellulases (2015) (0)
Apo-structure of alpha2,3-sialyltransferase variant 1 from Pasteurella dagmatis (2015) (0)
Crystal structure of Escherichia coli periplasmic glucose-1-phosphatase H18D mutant (2020) (0)
Disaccharide phosphorylases: mechanistic diversity and applications in the glycosciences (2011) (0)
Optimization of Glucose‐l‐Phosphate Production Employing Glucan‐Phosphorylases in Continuous Enzyme Membrane Reactors (1996) (0)
Glycosyltransferase mechanisms: lessons from phosphorylases. (2005) (0)
Influence of accelerated stress conditions on aggregation and process stability of human growth hormone (2011) (0)
Reaction Engineering Aspects of α-1,4- d -Glucan Phosphorylase Catalysis (1997) (0)
C-Glycosyltransferases: From mechanistic insight to synthetic application in flavonoid glycosylation (2015) (0)
NMR studies of xylose reductase catalysed transformations (2011) (0)
Metabolic engineering from quick and dirty to clean but slow – lessons from kinetic modelling of the xylose assimilation pathway (2013) (0)
Insight into the mechanism of C-glycosidic bond formation (2011) (0)
The reaction mechanism of D-mannitol 2-dehydrogenase from Pseudomonas fluorescens (PsM2DH) reveals convergent evolution of two alcohol dehydrogenase superfamilies. (2003) (0)
Prokaryotic fucosyltransferases for biotechnological production of fucosylated human milk oligosaccharides (2016) (0)
QbD Compliant Development of a Freeze/Thaw Process – From Formulation Development to Large Scale Freezing (2013) (0)
Identification, cloning, expression and characterization of a novel multifunctional sialyltransferase from Pasteurella dagmatis (2012) (0)
Role of an active site histidine (His-113) of xylose reductase from Candida tenuis (AKR 2B5) in catalysis and substrate specificity (2004) (0)
Chiral Resolution Through Stereoselective Transglycosylation by Sucrose Phosphorylase: Application to the Synthesis of a New Biomimetic Compatible Solute, (R)‐2‐O‐α‐D‐Glucopyranosyl Glyceric Acid Amide. (2014) (0)
Hydrolysis of cellulose by cellulases from Trichoderma reesei: enzyme adsorption and synergism (1993) (0)
The role of aromatic interactions at the catalytic subsite of sucrose phosphorylase (2011) (0)
Human Growth Hormone – Insights into its Aggregation Behavior using Denaturation Pathway Characterization (2010) (0)
twoendocellulases andtheir coreproteins on filter paperandtheir relation tohydrolysis (1994) (0)
Optimization and scale up of biocatalytic processes:Catalyst production, whole cell reduction and downstream processing (2011) (0)
Development of a microreactor for enzymatic processes (2006) (0)
Towards efficient flavonoid glycosylation through in situ sugar donor recycling (2015) (0)
The Essential Functional Interplay of the Catalytic Groups in Acid Phosphatase (2021) (0)
Crystal structure of human UDP-glucose dehydrogenase Thr131Ala, apo form. (2009) (0)
Molecular mechanisms of glucosyl transfer in retaining disaccharide phosphorylases (2008) (0)
Mechanisms of inactivation of the non-heme Fe2+-dependent dioxygenase Dke1 (2009) (0)
Use of ionic liquids to improve transglycosylation reactions of the hyperthermostable enzyme CelB from Pyrococcus furiosus (2005) (0)
Use of recombinant sucrose phosphorylase from Leuconostoc mesenteroides for the synthesis of alpha-glucosides (2004) (0)
Editorial Board: Biotechnology Journal 1/2019 (2019) (0)

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