Howard England Zalkin

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Howard England Zalkin

Biology

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Molecular Biology

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#1184

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Biochemistry

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#1432

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Chemistry
Biology

Howard England Zalkin's Degrees

PhD Biochemistry University of California, Berkeley
Masters Chemistry Stanford University

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Howard England Zalkin's Published Works

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Published Works

Crystal structure of LacI member, PurR, bound to DNA: minor groove binding by alpha helices. (1994) (359)
Lipide peroxidation in isolated mitochondria (1959) (269)
Inhibition of lipide peroxidation in mitochondria by vitamin E (1959) (256)
Enzymes utilizing glutamine as an amide donor. (1998) (256)
Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis. (1987) (236)
Structure of the allosteric regulatory enzyme of purine biosynthesis. (1994) (208)
Studies of the mechanism of vitamin E action. IV. Lipide peroxidation in the vitamin E-deficient rabbit. (1960) (206)
Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site. (1997) (176)
Yeast gene TRP5: structure, function, regulation. (1982) (173)
Increased lysosomal enzymes in muscular dystrophy of vitamin E-deficient rabbits. (1962) (146)
Transmethylation reactions in bacterial lipids (1963) (145)
Increased lysosomal enzymes in genetic muscular dystrophy. (1962) (144)
Role of primary structure and disulfide bond formation in beta-lactamase secretion (1983) (132)
Escherichia coli gene purR encoding a repressor protein for purine nucleotide synthesis. Cloning, nucleotide sequence, and interaction with the purF operator. (1988) (131)
Partial resolution of the enzymes catalyzing oxidative phosphorylation. V. Properties of coupling factor 4. (1965) (126)
De novo purine nucleotide biosynthesis. (1992) (125)
A mutation in adenylosuccinate lyase associated with mental retardation and autistic features (1992) (109)
Inhibition of Lipid Peroxidation in Microsomes by Vitamin E (1960) (104)
Nucleotide sequence of Escherichia coli pyrG encoding CTP synthetase. (1986) (97)
Mechanism of corepressor-mediated specific DNA binding by the purine repressor (1995) (96)
Unsaturated lipid peroxidation catalyzed by hematin compounds and its inhibition by vitamin E1 (1961) (96)
Enzymatic synthesis of cyclopropane fatty acids catalyzed by bacterial extracts. (1963) (93)
Identification of the Bacillus subtilis pur operon repressor. (1995) (92)
Nucleotide sequence of Saccharomyces cerevisiae genes TRP2 and TRP3 encoding bifunctional anthranilate synthase: indole-3-glycerol phosphate synthase. (1984) (90)
De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementation in E. coli. (1990) (83)
Crystal structure of glutamine phosphoribosylpyrophosphate amidotransferase from Escherichia coli (1998) (81)
Glutamine phosphoribosylpyrophosphate amidotransferase from Escherichia coli. Purification and properties. (1979) (80)
Structure and Function of the Glutamine Phosphoribosylpyrophosphate Amidotransferase Glutamine Site and Communication with the Phosphoribosylpyrophosphate Site* (1996) (80)
Regulated in vitro synthesis of Escherichia coli tryptophan operon messenger ribonucleic acid and enzymes. (1974) (76)
A cysteine-histidine-aspartate catalytic triad is involved in glutamine amide transfer function in purF-type glutamine amidotransferases. (1989) (76)
Nucleotide sequence of Escherichia coli purF and deduced amino acid sequence of glutamine phosphoribosylpyrophosphate amidotransferase. (1982) (74)
Crystal structure of Bacillus subtilis YabJ, a purine regulatory protein and member of the highly conserved YjgF family. (1999) (72)
Purification of the Escherichia coli purine regulon repressor and identification of corepressors (1990) (67)
Structural characterization and corepressor binding of the Escherichia coli purine repressor (1992) (67)
Binding of cytosolic aconitase to the iron responsive element of porcine mitochondrial aconitase mRNA. (1992) (67)
Formyltetrahydrofolate hydrolase, a regulatory enzyme that functions to balance pools of tetrahydrofolate and one-carbon tetrahydrofolate adducts in Escherichia coli (1995) (66)
Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction (1990) (64)
Correlation of genes and enzymes, and studies on regulation of the aromatic pathway in Salmonella. (1967) (64)
A Role for a Highly Conserved Protein of Unknown Function in Regulation of Bacillus subtilis purA by the Purine Repressor (1999) (63)
Mechanism of inactivation of glutamine amidotransferases by the antitumor drug L-(alpha S, 5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid (AT-125). (1980) (63)
Glutamine nucleotide sequence of Saccharomyces cerevisiae ADE4 encoding phosphoribosylpyrophosphate amidotransferase. (1984) (62)
Membrane-bound and soluble extracellular alpha-amylase from Bacillus subtilis. (1979) (61)
Repression of Escherichia coli purB is by a transcriptional roadblock mechanism (1992) (59)
Cloning and sequence of Bacillus subtilis purA and guaA, involved in the conversion of IMP to AMP and GMP (1992) (58)
Evidence that the iron-sulfur cluster of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase determines stability of the enzyme to degradation in vivo. (1989) (57)
Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase. (1985) (56)
The anthranilate synthetase-anthranilate-5-phosphorribosylpyrophosphate phosphoribosyltransferase aggregate. On the reaction mechanism of anthranilate synthetase from Salmonella typhimurium. (1970) (56)
Cloning and structural characterization of porcine heart aconitase. (1990) (55)
Regulation of Escherichia coli glnB, prsA, and speA by the purine repressor (1993) (55)
Regulation of the Escherichia coli glyA gene by the purR gene product (1990) (54)
Structural role for a conserved region in the CTP synthetase glutamine amide transfer domain (1987) (54)
Anthranilate synthetase. Purification and properties of component I from Salmonella typhimurium. (1968) (54)
Extracellular and membrane-bound proteases from Bacillus subtilis (1980) (53)
The anthranilate synthetase-anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase aggregate. Purification of the aggregate and regulatory properties of anthranilate synthetase. (1970) (53)
Expression, purification, and kinetic characterization of recombinant human adenylosuccinate lyase. (1993) (53)
Autoregulation of Escherichia coli purR requires two control sites downstream of the promoter (1990) (52)
Chorismate mutase-prephenate dehydratase. Partial purification and properties of the enzyme from Salmonella typhimurium. (1969) (52)
Dual Role for the Glutamine Phosphoribosylpyrophosphate Amidotransferase Ammonia Channel (2000) (51)
Organization and regulation of genes encoding biosynthetic enzymes in Bacillus subtilis. (1988) (51)
Glutamate synthase. Properties of the glutamine-dependent activity. (1976) (50)
Identification of a trpG-related glutamine amide transfer domain in Escherichia coli GMP synthetase. (1985) (50)
Mechanism of the synergistic end-product regulation of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase by nucleotides. (1997) (49)
Interaction of a putative repressor protein with an extended control region of the Bacillus subtilis pur operon. (1989) (48)
The amino acid sequence of D-ribose-binding protein from Escherichia coli K12. (1983) (48)
Mutational analysis of active site residues in pig heart aconitase. (1992) (47)
Cloning of the Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase gene in Escherichia coli. Nucleotide sequence determination and properties of the plasmid-encoded enzyme. (1983) (47)
purU, a source of formate for purT-dependent phosphoribosyl-N-formylglycinamide synthesis (1993) (45)
Cloning of a chicken liver cDNA encoding 5-aminoimidazole ribonucleotide carboxylase and 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase by functional complementation of Escherichia coli pur mutants. (1990) (45)
Studies of the mechanism of vitamin E action. V. Selenite and tocopherol inhibition of lipid peroxidation in the chick. (1960) (45)
Glutamine phosphoribosylpyrophosphate amidotransferase from cloned Escherichia coli purF. NH2-terminal amino acid sequence, identification of the glutamine site, and trace metal analysis. (1982) (44)
Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR (1992) (43)
Anthranilate Synthetase from Serratia marcescens ON THE PROPERTIES AND RELATIONSHIP TO THE ENZYME FROM SALMONELLA TYPHIMURIUM (1971) (42)
Regulation of Escherichia coli pyrC by the purine regulon repressor protein (1990) (41)
Bacillus subtilis pur operon expression and regulation (1989) (40)
The anthranilate synthetase-anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase aggregate. Catalytic and regulatory properties of aggregated and unaggregated forms of anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase. (1970) (40)
Detection of pur operon-attenuated mRNA and accumulated degradation intermediates in Bacillus subtilis. (1988) (40)
On the composition of anthranilate synthetase-anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase from Salmonella typhimurium. (1971) (39)
Replacement by site-directed mutagenesis indicates a role for histidine 170 in the glutamine amide transfer function of anthranilate synthase. (1985) (39)
Functional Dissection of the Bacillus subtilis pur Operator Site (2003) (39)
De novo purine nucleotide biosynthesis: cloning, sequencing and expression of a chicken PurH cDNA encoding 5-aminoimidazole-4-carboxamide-ribonucleotide transformylase-IMP cyclohydrolase. (1991) (38)
Nucleotide sequence of the guaA gene encoding GMP synthetase of Escherichia coli K12. (1985) (37)
Binding of purine nucleotides to two regulatory sites results in synergistic feedback inhibition of glutamine 5-phosphoribosylpyrophosphate amidotransferase. (1994) (36)
Anthranilate synthetase component II from Pseudomonas putida. Covalent structure and identification of the cysteine residue involved in catalysis. (1978) (35)
Two genes for de novo purine nucleotide synthesis on human chromosome 4 are closely linked and divergently transcribed. (1994) (35)
Coexpression of two closely linked avian genes for purine nucleotide synthesis from a bidirectional promoter (1993) (34)
Partial resolution of the enzymes catalyzing oxidative phosphorylation. IV. Formation of a complex between coupling factor 1 and adenosine diphosphate and its relation to the 14C-adenosine diphosphate-adenosine triphosphate exchange reaction. (1965) (34)
Analysis of the Chicken GPAT/AIRC Bidirectional Promoter for de Novo Purine Nucleotide Synthesis (*) (1995) (34)
Multiple forms of anthranilate synthetase-anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase from Salmonella typhimurium. (1971) (32)
The Purine Repressor of Bacillus subtilis: a Novel Combination of Domains Adapted for Transcription Regulation (2003) (31)
Characterization of the glutamyl-tRNA(Gln)-to-glutaminyl-tRNA(Gln) amidotransferase reaction of Bacillus subtilis (1988) (31)
Cloning of a cDNA encoding adenylosuccinate lyase by functional complementation in Escherichia coli. (1990) (31)
Characterization of the glutamine site of Escherichia coli guanosine 5'-monophosphate synthetase. (1977) (31)
Regulation of Escherichia coli purF. Mutations that define the promoter, operator, and purine repressor gene. (1988) (30)
Interaction of Bacillus subtilis purine repressor with DNA (1997) (30)
Role of the purine repressor hinge sequence in repressor function (1994) (29)
Properties of apoglutamate synthase and comparison with glutamate dehydrogenase. (1976) (29)
Active Subunits of Escherichia coli Glutamate Synthase (1976) (29)
Study of anthranilate synthase function by replacement of cysteine 84 using site-directed mutagenesis. (1985) (28)
Chorismate mutase-prephenate dehydratase. Evidence for distinct catalytic and regulatory sites. (1970) (28)
Role of NRF-1 in bidirectional transcription of the human GPAT-AIRC purine biosynthesis locus. (1997) (27)
Tyrosine-inhibited 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase. Properties of the partially purified enzyme from Salmonella typhimurium. (1970) (27)
[36] CTP synthetase (1985) (27)
Utilization of ammonia for tryptophan synthesis. (1975) (26)
Regulation of Escherichia coli purF. Analysis of the control region of a pur regulon gene. (1985) (26)
Identification of sites for feedback regulation of glutamine 5-phosphoribosylpyrophosphate amidotransferase by nucleotides and relationship to residues important for catalysis. (1993) (25)
Mutations in the Bacillus subtilis Purine Repressor That Perturb PRPP Effector Function In Vitro and In Vivo (2000) (24)
The amidotransferases. (1993) (24)
Purine, pyrimidine and pyridine nucleotide metabolism (2002) (24)
The structure of PurR mutant L54M shows an alternative route to DNA kinking (1998) (24)
[39] NAD synthetase (1985) (23)
Regulation of Escherichia coli purA by purine repressor, one component of a dual control mechanism (1994) (23)
Some physicochemical properties of anthranilate synthetase component I from Salmonella typhimurium. (1970) (22)
Tryptophan fluorescence monitors multiple conformational changes required for glutamine phosphoribosylpyrophosphate amidotransferase interdomain signaling and catalysis. (1999) (22)
Primary structure of Serratia marcescens anthranilate synthase component II. (1980) (22)
Interdomain Signaling in Glutamine Phosphoribosylpyrophosphate Amidotransferase* (1999) (21)
[32] Glutamine amidotransferases (1985) (21)
[35] Glucosamine-6-phosphate synthase (1985) (21)
Cloning and expression of avian glutamine phosphoribosylpyrophosphate amidotransferase. Conservation of a bacterial propeptide sequence supports a role for posttranslational processing. (1990) (21)
Anthranilate synthetase. (1973) (21)
Glutamine amidotransferase function. Replacement of the active-site cysteine in glutamine phosphoribosylpyrophosphate amidotransferase by site-directed mutagenesis. (1984) (20)
Chorismate mutase-prephenate dehydratase. Phenylalanine-induced dimerization and its relationship to feedback inhibition. (1971) (19)
Role of NifS in maturation of glutamine phosphoribosylpyrophosphate amidotransferase (1997) (19)
The glutamine-utilizing site of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase. (1983) (18)
Escherichia coli purine repressor: key residues for the allosteric transition between active and inactive conformations and for interdomain signaling. (1998) (17)
Avian glutamine phosphoribosylpyrophosphate amidotransferase propeptide processing and activity are dependent upon essential cysteine residues. (1992) (17)
Immunological study of anthranilate synthetase (1975) (16)
Role of residue 147 in the gene regulatory function of the Escherichia coli purine repressor. (2002) (16)
[37] Anthranilate synthase (1985) (16)
Cloning and characterization of the cDNA encoding human adenylosuccinate synthetase (1992) (15)
Mutagenesis of ligands to the [4 Fe-4S] center of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase. (1986) (15)
[42] Assay for 3-deoxy-d-arabino-heptulosonic acid 7-phosphate synthase (1970) (15)
Properties of anthranilate synthetase component II from Pseudomonas putida. (1976) (15)
Structure, function, and regulation of amidophosphoribosyltransferase from prokaryotes. (1983) (15)
Immunological cross-reactivity of Escherichia coli anthranilate synthetase, glutamate synthase, and other proteins. (1978) (14)
Chemical modifications of Serratia marcescens anthranilate synthase component I. (1981) (13)
Mutational analysis of the glutamine phosphoribosylpyrophosphate amidotransferase pro-peptide. (1988) (13)
Structure-based redesign of corepressor specificity of the Escherichia coli purine repressor by substitution of residue 190. (1998) (13)
Temperature-Dependent Function of the Glutamine Phosphoribosylpyrophosphate Amidotransferase Ammonia Channel and Coupling with Glycinamide Ribonucleotide Synthetase in a Hyperthermophile (2000) (13)
Affinity labeling of a glutamyl peptide in the coenzyme binding site of NADP+-specific glutamate dehydrogenase of Salmonella typhimurium by 2-[(4-bromo-2,3-dioxobutyl)thio]-1,N6-ethenoadenosine 2',5'-bisphosphate. (1989) (13)
Mutagenesis of amino acid residues required for binding of corepressors to the purine repressor. (1994) (12)
NAD synthetase. (1985) (12)
A Positively Charged Residue Bound in the Minor Groove Does not Alter the Bending of a DNA Duplex (1996) (12)
Amino-terminal deletions define a glutamine amide transfer domain in glutamine phosphoribosylpyrophosphate amidotransferase and other PurF-type amidotransferases (1990) (12)
Control of aromatic amino acid biosynthesis in Salmonella typhimurium (1967) (11)
Fine structure analysis of the Chinese hamster AS gene encoding asparagine synthetase. (1989) (11)
Modification of Serratia marcescens anthranilate synthase with pyridoxal 5'-phosphate. (1982) (10)
Crystallization and preliminary X-ray analysis of an Escherichia coli purine repressor-hypoxanthine-DNA complex. (1994) (10)
Importance of lysine-286 at the NADP site of glutamate dehydrogenase from Salmonella typhimurium. (1992) (10)
Glucosamine-6-phosphate synthase. (1985) (10)
Anthranilate synthase. (1985) (10)
[34] GMP Synthetase (1985) (9)
Roles of the TGACT repeat sequence in the yeast TRP5 promoter. (1987) (9)
Regulation of the Tyrosine Biosynthetic Enzymes in Salmonella typhimurium: Analysis of the Involvement of Tyrosyl-Transfer Ribonucleic Acid and Tyrosyl-Transfer Ribonucleic Acid Synthetase (1972) (8)
Mutational Analysis of Bacillus subtilisGlutamine Phosphoribosylpyrophosphate Amidotransferase Propeptide Processing (1999) (8)
Chemical modification and ligand binding studies with Escherichia coli glutamate synthase. (1983) (8)
Deletion mapping the yeast TRP5 control region. (1985) (8)
Overview of multienzyme systems in biosynthetic pathways. (1993) (8)
Isolation of Saccharomyces cerevisiae TRP3 (1983) (7)
An investigation of imine formation in the isocitrate dehydrogenase reaction. (1966) (7)
Evaluation of cysteine 283 and glutamic acid 284 in the coenzyme binding site of Salmonella typhimurium glutamate dehydrogenase by site-directed mutagenesis and reaction with the nucleotide analogue 2-[4-bromo-2,3-dioxobutyl)thio)-1,N6-ethenoadenosine 2',5'-bisphosphate. (1991) (7)
Glutamine amidotransferases. (1985) (7)
Amino acid sequence of a peptide containing an essential cysteine residue of Escherichia coli GMP synthetase. (1978) (5)
Crystallization and preliminary X-ray studies on the co-repressor binding domain of the Escherichia coli purine repressor. (1992) (5)
Anthranilate synthetase from Serratia marcescens. On the mechanism of tryptophan inhibition. (1972) (5)
synthesis . phosphoribosyl-N-formylglycinamide purU , a source of formate for purT-dependent (2003) (4)
CTP synthetase. (1985) (4)
Tryptophan-mediated substrate inhibition of anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase. (1969) (4)
Reaction of the nucleotide analogue 2-[(4-bromo-2,3-dioxobutyl)thio]adenosine 2',5'-bisphosphate at the coenzyme site of wild-type and mutant NADP(+)-specific glutamate dehydrogenases from Salmonella typhimurium. (1992) (3)
Increased lysosomal enzymes in muscular dystrophy. Abstr. (1961) (3)
Organization and regulation of genes for de novo purine nucleotide synthesis in Bacillus subtilis. (1991) (3)
STRUCTURE OF THE PURR-GUANINE-PURF OPERATOR COMPLEX (1997) (2)
The Anthranilate Synthetase-Anthrailate 5 = Phospho-ribosylpyrophosphate Phosphoribosyltransferase Aggregate (2002) (2)
p-Aminobenzoate synthase. (1985) (2)
CRYSTAL STRUCTURE OF THE LACI FAMILY MEMBER, PURR, BOUND TO DNA: MINOR GROOVE BINDING BY ALPHA HELICES (1998) (2)
Mechanism of the anthranilate synthetase reaction. Evidence against an intramolecular hydrogen transfer. (1970) (2)
CHARACTERIZATION OF THE BACILLUS SUBTILIS Pur OPERON: NEW INSIGHTS INTO GENE REGULATION (1988) (2)
[40] Glu-tRNAGln amidotransferase (1985) (2)
Mutations in PurBox1 of the Bacillus subtilis pur operon control site affect adenine-regulated expression in vivo. (2005) (1)
Regulation of Purine Nucleotide Synthesis (2000) (1)
Formyltetrahydrofolate hydrolase from Escherichia coli. (1997) (1)
Bacillus subtilis YABJ (1999) (0)
[85] Coupling factor 4 (F4) (1967) (0)
Glutamine Amidotransferase Function (2001) (0)
64 CONTROLLABLE EXPRESSION OF E.COLI AMIDOPHOSPHORIBOSYLTRANSFERASE (ATase) GENE IN ATase-DEFICIENT MAMMALIAN FIBROBLASTS-A BASIC MODEL FOR GENE THERAPY (1988) (0)
Affinity Labeling of Glutamine Amidotransferases by AT - (2001) (0)
STRUCTURE OF THE PURR MUTANT, L54M, BOUND TO HYPOXANTHINE AND PURF OPERATOR DNA (1998) (0)
Glu-tRNAGln amidotransferase. (1985) (0)
Morphophonemic alternations in the Serbocroatian inflectional system (1979) (0)
Nucleotide-bound Structures of Bacillus subtilis Glycinamide Ribonucleotide Synthetase (2011) (0)
Studies on aconitase by spectroscopy, crystallography and mutagenesis (1991) (0)
Bacillus subtilis pur Operon Expression and Regulationt (0)
Controllable expression of an E. coli amidophosphoribosyltransferase (ATase) gene in ATase-deficient mammalian fibroblasts--a basic model for gene therapy. (1989) (0)
DNA-BINDING REGULATORY PROTEIN (1996) (0)
Mutations in PurBox1 of theBacillus subtilis pur operon control site affect adenine-regulated expressionin vivo (2008) (0)
Purine Repressor Mutant Corepressor Binding Domain Structure (2002) (0)
Anthranilate Synthetase Component II from Pseudomonas putida (2011) (0)
PURINE REPRESSOR MUTANT-HYPOXANTHINE-PURF OPERATOR COMPLEX (2002) (0)
CRYSTAL STRUCTURE OF THE PURINE OPERON REPRESSOR OF BACILLUS SUBTILIS (2003) (0)
Crystal Structure of the PurR complexed with cPRPP (2003) (0)
Structural and functional studies on Bacillus subtilis YabJ, a member of the highly conserved YjgF family (2000) (0)
The Genetic Basis of ASase Deficiency (1993) (0)
Evidence that the 4 iron 4 sulfur cluster of bacillus subtilis glutamine prpp amidotransferase controls the stability of the enzyme in vitro (1988) (0)
GenesoftheEscherichia coli pur Regulon AreNegatively Controlled byaRepressor-Operator (1990) (0)
PURINE REPRESSOR-HYPOXANTHINE-PURF-OPERATOR COMPLEX (1995) (0)
GMP synthetase. (1985) (0)
Cloning and Sequence of Bacillus subtilis purA and guaA, Involved in the Conversion of IMP to AMP and GMPt (0)

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