Holger Puchta
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Molecular Biology
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Biochemistry
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Biology
Holger Puchta's Degrees
- PhD Molecular Biology University of Cologne
- Masters Biochemistry University of Cologne
- Bachelors Biology University of Cologne
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(Suggest an Edit or Addition)Holger Puchta's Published Works
Number of citations in a given year to any of this author's works
Total number of citations to an author for the works they published in a given year. This highlights publication of the most important work(s) by the author
Published Works
- Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. (2014) (601)
- The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution. (2004) (549)
- Elevated UV-B radiation reduces genome stability in plants (2000) (374)
- Two different but related mechanisms are used in plants for the repair of genomic double-strand breaks by homologous recombination. (1996) (372)
- Capture of genomic and T‐DNA sequences during double‐strand break repair in somatic plant cells (1998) (366)
- The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny. (2014) (301)
- Homologous recombination in plant cells is enhanced by in vivo induction of double strand breaks into DNA by a site-specific endonuclease. (1993) (267)
- Applying CRISPR/Cas for genome engineering in plants: the best is yet to come. (2017) (227)
- Synthetic nucleases for genome engineering in plants: prospects for a bright future. (2014) (212)
- Highly efficient heritable plant genome engineering using Cas9 orthologues from Streptococcus thermophilus and Staphylococcus aureus. (2015) (211)
- Intrachromosomal homologous recombination in whole plants. (1994) (197)
- Species‐specific double‐strand break repair and genome evolution in plants (2000) (197)
- Towards CRISPR/Cas crops - bringing together genomics and genome editing. (2017) (195)
- In planta gene targeting (2012) (190)
- Gene targeting in plants: 25 years later. (2013) (159)
- Elimination of selection markers from transgenic plants. (2001) (153)
- Efficient Repair of Genomic Double-Strand Breaks by Homologous Recombination between Directly Repeated Sequences in the Plant Genome Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.001727. (2002) (152)
- Gene replacement by homologous recombination in plants (2004) (149)
- Molecular characterisation of two paralogous SPO11 homologues in Arabidopsis thaliana. (2000) (144)
- Two closely related RecQ helicases have antagonistic roles in homologous recombination and DNA repair in Arabidopsis thaliana (2007) (142)
- Agrobacterium tumefaciens transfers single-stranded transferred DNA (T-DNA) into the plant cell nucleus. (1994) (137)
- The Catalytically Active Tyrosine Residues of Both SPO11-1 and SPO11-2 Are Required for Meiotic Double-Strand Break Induction in Arabidopsis (2007) (129)
- Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas (2016) (129)
- Induction of intrachromosomal homologous recombination in whole plants (1995) (125)
- An Archaebacterial Topoisomerase Homolog Not Present in Other Eukaryotes Is Indispensable for Cell Proliferation of Plants (2002) (122)
- Repair of genomic double‐strand breaks in somatic plant cells by one‐sided invasion of homologous sequences (1998) (116)
- Molecular characterization of homologues of both subunits A (SPO11) and B of the archaebacterial topoisomerase 6 in plants. (2001) (115)
- Different pathways of homologous recombination are used for the repair of double-strand breaks within tandemly arranged sequences in the plant genome. (2003) (113)
- RecA stimulates sister chromatid exchange and the fidelity of double-strand break repair, but not gene targeting, in plants transformed by Agrobacterium. (2000) (112)
- The role of AtMUS81 in DNA repair and its genetic interaction with the helicase AtRecQ4A (2006) (110)
- Marker-free transgenic plants (2003) (109)
- Removing selectable marker genes: taking the shortcut. (2000) (105)
- The STRUCTURAL MAINTENANCE OF CHROMOSOMES 5/6 Complex Promotes Sister Chromatid Alignment and Homologous Recombination after DNA Damage in Arabidopsis thaliana[C][W] (2009) (101)
- The transcriptional response of Arabidopsis to genotoxic stress - a high-density colony array study (HDCA). (2003) (101)
- Plant breeding at the speed of light: the power of CRISPR/Cas to generate directed genetic diversity at multiple sites (2019) (99)
- Live‐cell CRISPR imaging in plants reveals dynamic telomere movements (2017) (98)
- Efficient in planta gene targeting in Arabidopsis using egg cell‐specific expression of the Cas9 nuclease of Staphylococcus aureus (2018) (97)
- Topoisomerase 3α and RMI1 Suppress Somatic Crossovers and Are Essential for Resolution of Meiotic Recombination Intermediates in Arabidopsis thaliana (2008) (95)
- The Fanconi Anemia Ortholog FANCM Ensures Ordered Homologous Recombination in Both Somatic and Meiotic Cells in Arabidopsis[W] (2012) (95)
- Double-strand break-induced recombination between ectopic homologous sequences in somatic plant cells. (1999) (93)
- From classical mutagenesis to nuclease-based breeding - directing natural DNA repair for a natural end-product. (2017) (92)
- CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination (2019) (92)
- Molecular characterisation of RecQ homologues in Arabidopsis thaliana. (2000) (91)
- RAD5A, RECQ4A, and MUS81 Have Specific Functions in Homologous Recombination and Define Different Pathways of DNA Repair in Arabidopsis thaliana[W] (2010) (90)
- DNA recombination in somatic plant cells: mechanisms and evolutionary consequences (2014) (83)
- Effects of nanosecond pulsed electric field exposure on arabidopsis thaliana (2009) (81)
- Using CRISPR/Cas in three dimensions: towards synthetic plant genomes, transcriptomes and epigenomes. (2016) (80)
- CRISPR–Cas9-mediated induction of heritable chromosomal translocations in Arabidopsis (2020) (80)
- The Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination. (2004) (79)
- CRISPR/Cas brings plant biology and breeding into the fast lane. (2020) (79)
- Homology-based double-strand break-induced genome engineering in plants (2016) (77)
- The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology. (2018) (75)
- Engineering CRISPR/LbCas12a for highly efficient, temperature‐tolerant plant gene editing (2019) (75)
- The role of DNA helicases and their interaction partners in genome stability and meiotic recombination in plants. (2011) (71)
- The molecular structure of hop latent viroid (HLV), a new viroid occurring worldwide in hops. (1988) (70)
- The CRISPR/Cas revolution reaches the RNA world: Cas13, a new Swiss Army knife for plant biologists (2018) (69)
- Gene regulation in response to DNA damage. (2012) (68)
- The requirement for recombination factors differs considerably between different pathways of homologous double-strand break repair in somatic plant cells. (2012) (68)
- Somatic intrachromosomal homologous recombination events in populations of plant siblings (1995) (67)
- The RecQ gene family in plants. (2006) (66)
- Primary and secondary structure of citrus viroid IV (CVd IV), a new chimeric viroid present in dwarfed grapefruit in Israel. (1991) (64)
- Two Unlinked Double-Strand Breaks Can Induce Reciprocal Exchanges in Plant Genomes via Homologous Recombination and Nonhomologous End Joining (2007) (63)
- Transforming plant biology and breeding with CRISPR/Cas9, Cas12 and Cas13 (2018) (62)
- Efficient induction of heritable inversions in plant genomes using the CRISPR/Cas system. (2019) (60)
- In planta gene targeting can be enhanced by the use of CRISPR/Cas12a. (2019) (57)
- A transient assay in plant cells reveals a positive correlation between extrachromosomal recombination rates and length of homologous overlap. (1991) (57)
- BRCA2 is a mediator of RAD51- and DMC1-facilitated homologous recombination in Arabidopsis thaliana. (2012) (56)
- Gene therapy in plants. (1999) (56)
- Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering (2020) (54)
- A homologue of the breast cancer‐associated gene BARD1 is involved in DNA repair in plants (2006) (54)
- Towards targeted transformation in plants (1998) (52)
- Differences in the processing of DNA ends in Arabidopsis thaliana and tobacco: possible implications for genome evolution (2003) (51)
- Repair of adjacent single-strand breaks is often accompanied by the formation of tandem sequence duplications in plant genomes (2016) (51)
- ZYP1 is required for obligate cross-over formation and cross-over interference in Arabidopsis (2021) (49)
- A Homolog of ScRAD5 Is Involved in DNA Repair and Homologous Recombination in Arabidopsis1[W] (2008) (49)
- Fork sensing and strand switching control antagonistic activities of RecQ helicases (2013) (48)
- Intron gain and loss in the evolution of the conserved eukaryotic recombination machinery. (2002) (48)
- A new strain of potato spindle tuber viroid (PSTVd-N) exhibits major sequence differences as compared to all other PSTVd strains sequenced so far (1990) (47)
- The role of double-strand break-induced allelic homologous recombination in somatic plant cells. (2002) (47)
- Two Distinct MUS81-EME1 Complexes from Arabidopsis Process Holliday Junctions1[W] (2009) (44)
- Towards the ideal GMP: homologous recombination and marker gene excision. (2003) (43)
- The Arabidopsis thaliana Homolog of the Helicase RTEL1 Plays Multiple Roles in Preserving Genome Stability[C][W] (2014) (42)
- Homologs of Breast Cancer Genes in Plants (2011) (41)
- Knocking out consumer concerns and regulator’s rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals (2017) (40)
- AtRECQ2, a RecQ helicase homologue from Arabidopsis thaliana, is able to disrupt various recombinogenic DNA structures in vitro. (2008) (40)
- In Planta Somatic Homologous Recombination Assay Revisited: A Successful and Versatile, but Delicate Tool (2012) (39)
- Defining the roles of the N-terminal region and the helicase activity of RECQ4A in DNA repair and homologous recombination in Arabidopsis (2013) (39)
- Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana (2015) (39)
- DNA Break Repair in Plants and Its Application for Genome Engineering. (2018) (36)
- BRCC36A is epistatic to BRCA1 in DNA crosslink repair and homologous recombination in Arabidopsis thaliana (2010) (34)
- Advances in New Technology for Targeted Modification of Plant Genomes (2015) (34)
- Some like it sticky: targeting of the rice gene Waxy. (2003) (34)
- Extrachromosomal homologous DNA recombination in plant cells is fast and is not affected by CpG methylation (1992) (33)
- Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering (2021) (33)
- From gene editing to genome engineering: restructuring plant chromosomes via CRISPR/Cas (2019) (31)
- Endogenous sequence patterns predispose the repair modes of CRISPR/Cas9‐induced DNA double‐stranded breaks in Arabidopsis thaliana (2017) (30)
- Efficient Agrobacterium-mediated transformation of Arabidopsis thaliana using the bar gene as selectable marker (1995) (30)
- The RTR complex as caretaker of genome stability and its unique meiotic function in plants (2014) (29)
- Use of I-Sce I to induce DNA double-strand breaks in Nicotiana. (1999) (28)
- Biochemical Characterization of an Exonuclease from Arabidopsis thaliana Reveals Similarities to the DNA Exonuclease of the Human Werner Syndrome Protein* (2003) (28)
- Enhancing in planta gene targeting efficiencies in Arabidopsis using temperature‐tolerant CRISPR/LbCas12a (2020) (27)
- CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases. (2016) (26)
- Nucleotide sequence of a hop stunt viroid (HSVd) isolate from grapefruit in Israel. (1989) (25)
- Different functions for the domains of the Arabidopsis thaliana RMI1 protein in DNA cross-link repair, somatic and meiotic recombination (2013) (25)
- The RTR Complex Partner RMI2 and the DNA Helicase RTEL1 Are Both Independently Involved in Preserving the Stability of 45S rDNA Repeats in Arabidopsis thaliana (2016) (25)
- Efficient gene targeting in Nicotiana tabacum using CRISPR/SaCas9 and temperature tolerant LbCas12a (2021) (25)
- MHF1 plays Fanconi anaemia complementation group M protein (FANCM)-dependent and FANCM-independent roles in DNA repair and homologous recombination in plants. (2014) (24)
- CRISPR–Cas-mediated chromosome engineering for crop improvement and synthetic biology (2021) (23)
- The mechanism of extrachromosomal homologous DNA recombination in plant cells (1991) (23)
- A SRS2 homolog from Arabidopsis thaliana disrupts recombinogenic DNA intermediates and facilitates single strand annealing (2009) (23)
- Development of Bag-1L as a therapeutic target in androgen receptor-dependent prostate cancer (2017) (22)
- Nucleotide sequence of a hop stunt viroid (HSVd) isolate from the German grapevine rootstock 5BB as determined by PCR-mediated sequence analysis. (1989) (21)
- The Protease WSS1A, the Endonuclease MUS81, and the Phosphodiesterase TDP1 Are Involved in Independent Pathways of DNA-protein Crosslink Repair in Plants (2019) (21)
- Breaking news: Plants mutate right on target (2010) (21)
- Nucleotide sequence of a hop stunt viroid isolate from the German grapevine cultivar 'Riesling'. (1988) (20)
- An Arabidopsis FANCJ helicase homologue is required for DNA crosslink repair and rDNA repeat stability (2019) (20)
- Green light for gene targeting in plants. (2005) (20)
- The topoisomerase 3α zinc-finger domain T1 of Arabidopsis thaliana is required for targeting the enzyme activity to Holliday junction-like DNA repair intermediates (2018) (19)
- Biochemical Characterization of AtRECQ3 Reveals Significant Differences Relative to Other RecQ Helicases1[W] (2009) (18)
- The RecQ-like helicase HRQ1 is involved in DNA crosslink repair in Arabidopsis in a common pathway with the Fanconi anemia-associated nuclease FAN1 and the postreplicative repair ATPase RAD5A. (2018) (17)
- Nucleotide sequence and secondary structure of apple scar skin viroid (ASSVd) from China (1990) (16)
- The nuclease FAN1 is involved in DNA crosslink repair in Arabidopsis thaliana independently of the nuclease MUS81 (2015) (16)
- Intrachromosomal homologous recombination in Arabidopsis thaliana. (2004) (16)
- Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants. (2018) (15)
- Emerging tools for synthetic biology in plants. (2014) (15)
- CRISPR/Cas-mediated chromosome engineering: opening up a new avenue for plant breeding. (2020) (15)
- The DNA translocase RAD5A acts independently of the other main DNA repair pathways, and requires both its ATPase and RING domain for activity in Arabidopsis thaliana (2017) (14)
- Substrate specificity of plant recombinases determined in extrachromosomal recombination systems. (1994) (13)
- Metal-mediated DNA assembly using the ethynyl linked terpyridine ligand. (2012) (13)
- DNA- and DNA-Protein-Crosslink Repair in Plants (2019) (13)
- The Translesion Polymerase ζ Has Roles Dependent on and Independent of the Nuclease MUS81 and the Helicase RECQ4A in DNA Damage Repair in Arabidopsis1 (2015) (13)
- Homologous recombination in plants (2005) (13)
- Purification and characterization of RecQ helicases of plants. (2010) (12)
- AtRAD5A is a DNA translocase harboring a HIRAN domain which confers binding to branched DNA structures and is required for DNA repair in vivo. (2016) (12)
- Use of the Cas9 Orthologs from Streptococcus thermophilus and Staphylococcus aureus for Non-Homologous End-Joining Mediated Site-Specific Mutagenesis in Arabidopsis thaliana. (2017) (12)
- DNA Repair and Recombination in Plants (2014) (12)
- Application of Aptamers Improves CRISPR-Based Live Imaging of Plant Telomeres (2020) (11)
- Double-Strand Break Repair and Its Application to Genome Engineering in Plants (2015) (11)
- Sequence analysis of minute amounts of viroid RNA using the polymerase chain reaction (PCR) (2005) (11)
- Nucleotide sequence of the Korean strain of hop stunt viroid (HSV). (1988) (10)
- DNA Helicases as Safekeepers of Genome Stability in Plants (2019) (10)
- The Rad50 genes of diploid and polyploid wheat species. Analysis of homologue and homoeologue expression and interactions with Mre11 (2011) (10)
- CRISPR/Cas-Mediated In Planta Gene Targeting. (2017) (9)
- What Comparative Genomics Tells Us About the Evolution of Eukaryotic Genes Involved in Recombination (2004) (9)
- Sophisticated CRISPR/Cas tools for fine-tuning plant performance. (2020) (8)
- Using CRISPR/ttLbCas12a for in planta Gene Targeting in A. thaliana. (2020) (8)
- The nuclease FAN 1 is involved in DNA crosslink repair in Arabidopsis thaliana independently of the nuclease MUS 81 (2015) (7)
- Molecular and biological properties of a cloned and infectious new sequence variant of cucumber pale fruit viroid (CPFV). (1988) (7)
- CRISPR–Cas9-mediated chromosome engineering in Arabidopsis thaliana (2022) (7)
- Broadening the applicability of CRISPR/Cas9 in plants (2017) (7)
- Repair of DNA-protein crosslinks in plants. (2020) (6)
- CRISPR Guide RNA Design Guidelines for Efficient Genome Editing. (2020) (6)
- Different functional roles of RTR complex factors in DNA repair and meiosis in Arabidopsis and tomato. (2021) (6)
- Genome engineering using CRISPR/Cas: getting more versatile and more precise at the same time (2016) (5)
- Analyzing Somatic DNA Repair in Arabidopsis Meiotic Mutants. (2020) (5)
- Breaking DNA in plants: how I almost missed my personal breakthrough. (2016) (5)
- The repair of topoisomerase 2 cleavage complexes in Arabidopsis. (2021) (4)
- A homologue of the breast cancer‐associated gene BARD1 is involved in DNA repair in plants (2013) (4)
- Massive crossover suppression by CRISPR–Cas-mediated plant chromosome engineering (2022) (4)
- Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats (2022) (4)
- Application of CRISPR/Cas-mediated base editing for directed protein evolution in plants (2020) (4)
- An improved procedure for the rapid one-step-cloning of full-length viroid cDNA (2005) (4)
- CRISPR/Cas-based genome engineering (2014) (4)
- The role of At MUS 81 in DNA repair and its genetic interaction with the helicase At RecQ 4 A (2006) (4)
- Live-Cell CRISPR Imaging in Plant Cells with a Telomere-Specific Guide RNA. (2020) (4)
- Genome Engineering mit CRISPR/Cas — Revolution in der Pflanzenzüchtung (2017) (3)
- Updates on gene editing and its applications. (2022) (3)
- Nucleus and Genome: DNA Recombination and Repair (2014) (3)
- Homology-based double-strand break-induced genome engineering in plants (2016) (2)
- Chromatin and development: a special issue. (2015) (2)
- Different DNA repair pathways are involved in single-strand break-induced genomic changes in plants. (2021) (2)
- Double strand break (DSB) repair pathways in plants and their application in genome engineering (2021) (2)
- Hop latent viroid (HLVd) and the worldwide distribution of latent viroids in vegetatively propagated plants (1991) (1)
- The Translesion Polymerase z Has Roles Dependent on and Independent of the Nuclease MUS 81 and the Helicase RECQ 4 A in DNA Damage Repair in Arabidopsis 1 (2015) (1)
- Representative measurement of the smallest dust concentrations in exhaust gas channels (1996) (1)
- Knocking out consumer concerns and regulator’s rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals (2017) (1)
- Getting better all the time - recent progress in the development of CRISPR/Cas-based tools for plant genome engineering. (2022) (1)
- Chromosome and Genomic Engineering in Plants (2016) (1)
- Role of human disease genes for the maintenance of genome stability in plants. (2009) (1)
- Biochemical characterization of RecQ helicases from Arabidopsis thaliana (2007) (1)
- Plant genome editing using engineered nucleases and success of CRISPR/Cas9 system (2017) (1)
- DNA-protein Crosslink Repair in Plants (2019) (1)
- Enhancing gene editing and gene targeting efficiencies in Arabidopsis thaliana by using an intron‐containing version of ttLbCas12a (2022) (1)
- Extrachromosomal Homologous DNA Recombination inPlant Cells IsFastandIsNotAffected byCpGMethylation (1992) (1)
- Double-strand break induced genome engineering in plants: Past, Present, Future (2017) (0)
- DNA repair meets climate change (2020) (0)
- The DNA-dependent Protease AtWSS1A suppresses persistent double strand break formation during replication. (2021) (0)
- DNA recombination in somatic plant cells: mechanisms and evolutionary consequences (2014) (0)
- Running head: Role of translesion polymerase ζ in DNA repair (2015) (0)
- Role of the Homolog of the Fanconi Anemia M Associated Histone Fold Complex Factor MHF1 in DNA Repair and DNA Recombination in Plants (2014) (0)
- Efficient Homologous Recombination-Mediated in Planta Gene Targeting by Egg-Cell-Specific Expression of Staphylococcus aureus Cas9 from Arabidopsis (2020) (0)
- Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas (2016) (0)
- DSB repair by illegitimate and homologous DNA recombination in Arabidopsis thaliana (2002) (0)
- Broadening the applicability of CRISPR/Cas9 in plants (2017) (0)
- Protein assisted assembly of superlattices (2010) (0)
- WITHDRAWN: DNA-protein crosslink repair in plants. (2020) (0)
- DNA Unwinding Mechanism of a RecQ Helicase from Arabidopsis Thaliana Investigated with Magnetic Tweezers (2011) (0)
- Freely Orbiting Magnetic Tweezers: A New Twist on Single Molecule Force Spectroscopy (2011) (0)
- Plant Symposia (2015) (0)
- HaploTools: Generation of Haploidyinducing Lines via Genome Engineering Novel Tools for an Accelerated Plant Breeding Process of Mono- and Eudicot Crops (2018) (0)
- The Role of the RTR Complex in Somatic Genome Stability in Plants (2014) (0)
- Plasma Physics Division ( P ) Overview Plasma Physics Division ( P ) (2010) (0)
- Plant breeding at the speed of light: the power of CRISPR/Cas to generate directed genetic diversity at multiple sites (2019) (0)
- Application of CRISPR/Cas-mediated base editing for directed protein evolution in plants (2020) (0)
- The role of At MUS81 in DNA repair and its genetic interaction with the helicase At RecQ4A (2006) (0)
- CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination (2019) (0)
- Genome engineering using CRISPR/Cas: getting more versatile and more precise at the same time (2016) (0)
- SMC5/6 complex-mediated SUMOylation stimulates DNA–protein cross-link repair in Arabidopsis (2023) (0)
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