Howard Ronald Kaback

Howard Ronald Kaback's AcademicInfluence.com Rankings

Biology

#2814

World Rank

#4402

Historical Rank

#1214

USA Rank

Microbiology

#100

World Rank

#122

Historical Rank

#44

USA Rank

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Biology

Why Is Howard Ronald Kaback Influential?

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According to Wikipedia, Howard Ronald Kaback was an American biochemist, known for Kabackosomes, the cell-free membrane transport vesicles. He was the brother of Michael M. Kaback, pediatrician and human geneticist, who developed a screening program to detect and prevent Tay–Sachs disease, a rare and fatal genetic disorder most common in Ashkenazi Jews.

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Howard Ronald Kaback's Published Works

Number of citations in a given year to any of this author's works

Total number of citations to an author for the works they published in a given year. This highlights publication of the most important work(s) by the author

Published Works

Structure and Mechanism of the Lactose Permease of Escherichia coli (2003) (1405)
Cloning and functional expression in bacteria of a novel glucose transporter present in liver, intestine, kidney, and β-pancreatic islet cells (1988) (662)
[13] Bacterial Membranes (1971) (450)
Lessons from lactose permease. (2006) (322)
Cys‐scanning mutagenesis: a novel approach to structure—function relationships in polytopic membrane proteins (1998) (311)
The kamikaze approach to membrane transport (2001) (265)
Membrane potential and active transport in membrane vesicles from Escherichia coli. (1975) (254)
NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain. (1987) (245)
Purification and reconstitution of functional lactose carrier from Escherichia coli. (1981) (244)
Structural determination of wild-type lactose permease (2007) (224)
Structure of the lac carrier protein of Escherichia coli. (1983) (221)
Transport Studies in Bacterial Membrane Vesicles (1974) (220)
Transport across isolated bacterial cytoplasmic membranes. (1972) (218)
The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles. (1976) (216)
Role of YidC in folding of polytopic membrane proteins (2004) (209)
Use of a lipophilic cation for determination of membrane potential in neuroblastoma-glioma hybrid cell suspensions. (1979) (201)
Mechanisms of active transport in isolated membrane vesicles. II. The mechanism of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in membrane preparations from Escherichia coli. (1971) (194)
Quantitative measurements of membrane potential in Escherichia coli. (1980) (191)
A Phospholipid Acts as a Chaperone in Assembly of a Membrane Transport Protein (*) (1996) (188)
Membranes and Transport (1973) (185)
Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY (2006) (183)
The relationship between the electrochemical proton gradient and active transport in Escherichia coli membrane vesicles. (1977) (173)
Cloning and functional expression in bacteria of a novel glucose transporter present in liver, intestine, kidney, and beta-pancreatic islet cells. (1988) (170)
The electrochemical proton gradient in Escherichia coli membrane vesicles. (1977) (170)
Sugar binding induces an outward facing conformation of LacY (2007) (169)
The Role of the Phosphoenolpyruvate-phosphotransferase System in the Transport of Sugars by Isolated Membrane Preparations of Escherichia coli (1968) (163)
Site-directed alkylation and the alternating access model for LacY (2007) (150)
Purification, reconstitution, and characterization of the lac permease of Escherichia coli. (1986) (149)
Single-molecule FRET reveals sugar-induced conformational dynamics in LacY (2007) (149)
In vivo expression of the lacY gene in two segments leads to functional lac permease. (1990) (148)
Mechanisms of active transport in isolated membrane vesicles. 2. The coupling of reduced phenazine methosulfate to the concentrative uptake of beta-galactosides and amino acids. (1971) (141)
Extracellular ATP perturbs transmembrane ion fluxes, elevates cytosolic [Ca2+], and inhibits phagocytosis in mouse macrophages. (1985) (140)
Membrane potential changes during mitogenic stimulation of mouse spleen lymphocytes. (1980) (138)
Localization of D-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles. (1975) (133)
Mechanisms of active transport in isolated membrane vesicles. I. The site of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in Escherichia coli membrane vesicles. (1971) (133)
Structure-based mechanism for Na+/melibiose symport by MelB (2014) (126)
Electrochemical proton gradient in inverted membrane vesicles from Escherichia coli. (1980) (124)
Membrane potential and gentamicin uptake in Staphylococcus aureus. (1982) (123)
Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 1. Effect of pH on efflux, exchange, and counterflow. (1979) (122)
Mechanisms of active transport in isolated bacterial membrane vesicles. 8. The transport of amino acids by membranes prepared from Escherichia coli. (1972) (122)
Mechanisms of active transport in isolated bacterial membrane vesicles. 18. The mechanism of action of carbonylcyanide m-chlorophenylhydrazone. (1974) (122)
Cytochrome o type oxidase from Escherichia coli. Characterization of the enzyme and mechanism of electrochemical proton gradient generation. (1984) (118)
Structure and mechanism of the lactose permease. (2005) (118)
Lactose permease and the alternating access mechanism. (2011) (113)
lac permease of Escherichia coli: histidine-322 and glutamic acid-325 may be components of a charge-relay system. (1986) (112)
The Alternating Access Transport Mechanism in LacY (2010) (112)
Functional interactions between putative intramembrane charged residues in the lactose permease of Escherichia coli. (1992) (112)
Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 2. Effect of imposed delata psi, delta pH, and Delta mu H+. (1979) (111)
[72] Transport in isolated bacterial membrane vesicles (1974) (110)
Molecular biology and energetics of membrane transport. (1976) (109)
Proline uptake by an isolated cytoplasmic membrane preparation of Escherichia coli. (1966) (109)
Sodium-dependent methyl 1-thio-beta-D-galactopyranoside transport in membrane vesicles isolated from Salmonella typhimurium. (1977) (109)
Structure of sugar-bound LacY (2014) (109)
Lactose permease as a paradigm for membrane transport proteins (Review) (2004) (108)
Use of site-directed fluorescence labeling to study proximity relationships in the lactose permease of Escherichia coli. (1993) (107)
Toward the bilayer proteome, electrospray ionization-mass spectrometry of large, intact transmembrane proteins. (1999) (106)
Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli. (1994) (106)
Anaerobic transport in Escherichia coli membrane vesicles. (1973) (99)
Opening and closing of the periplasmic gate in lactose permease (2008) (98)
The lactose permease of Escherichia coli: overall structure, the sugar‐binding site and the alternating access model for transport (2003) (98)
From membrane to molecule to the third amino acid from the left with a membrane transport protein (1997) (97)
Mechanisms of active transport in isolated bacterial membrane vesicles. XV. Purification and properties of the membrane-bound D-lactate dehydrogenase from Escherichia coli. (1973) (95)
Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition (2011) (93)
Intramolecular dislocation of the COOH terminus of the lac carrier protein in reconstituted proteoliposomes. (1984) (93)
Construction of a functional lactose permease devoid of cysteine residues. (1991) (92)
Role of the charge pair aspartic acid-237-lysine-358 in the lactose permease of Escherichia coli. (1993) (92)
Na+/H+ antiport in isolated plasma membrane vesicles from the halotolerant alga Dunaliella salina (1986) (89)
pH-dependent changes in proton:substrate stoichiometries during active transport in Escherichia coli membrane vesicles. (1977) (88)
A chemiosmotic mechanism of symport (2015) (87)
Characterization of site-directed mutants in the lac permease of Escherichia coli. 2. Glutamate-325 replacements. (1989) (87)
Lipophilic cations: a group of model substrates for the multidrug-resistance transporter. (1992) (86)
A method for distance determination in proteins using a designed metal ion binding site and site-directed spin labeling: evaluation with T4 lysozyme. (1995) (85)
Purified lac permease and cytochrome o oxidase are functional as monomers. (1987) (85)
An approach to membrane protein structure without crystals (2002) (85)
A mutation in the lactose permease of Escherichia coli that decreases conformational flexibility and increases protein stability. (2003) (83)
Transport processes in eukaryotic and prokaryotic organisms (1996) (81)
Cysteine scanning mutagenesis of putative transmembrane helices IX and X in the lactose permease of Escherichia coli (1993) (80)
Membrane topology of the melibiose permease of Escherichia coli studied by melB-phoA fusion analysis. (1996) (80)
Functional architecture of MFS d-glucose transporters (2014) (80)
Mechanism of monensin-induced hyperpolarization of neuroblastoma-glioma hybrid NG108-15. (1979) (79)
Two-dimensional crystallization of Escherichia coli lactose permease. (1999) (79)
Monoclonal antibodies against the lac carrier protein from Escherichia coli. 2. Binding studies with membrane vesicles and proteoliposomes reconstituted with purified lac carrier protein. (1984) (78)
Transport across isolated bacterial cytoplasmic membranes. (1972) (78)
Proteomics on full-length membrane proteins using mass spectrometry. (2000) (78)
Relationships of the structure and function of the interferon receptor to hormone receptors and establishment of the antiviral state. (1978) (78)
Mechanisms of active transport in isolated bacterial membrane vesicles. 8. Valinomycin-induced rubidium transport. (1973) (78)
Properties and purification of an active biotinylated lactose permease from Escherichia coli. (1993) (77)
Mechanism of lactose translocation in proteoliposomes reconstituted with lac carrier protein purified from Escherichia coli. 1. Effect of pH and imposed membrane potential on efflux, exchange, and counterflow. (1983) (76)
Charged Residues in Transmembrane Domains II and XI of a Vesicular Monoamine Transporter Form a Charge Pair That Promotes High Affinity Substrate Recognition* (1997) (75)
Evolutionary mix-and-match with MFS transporters (2013) (75)
Molecular structure of membrane vesicles from Escherichia coli. (1978) (74)
Active transport in Escherichia coli: passage to permease. (1986) (74)
The lipid bilayer determines helical tilt angle and function in lactose permease of Escherichia coli. (1997) (74)
Structural comparison of lactose permease and the glycerol-3-phosphate antiporter: members of the major facilitator superfamily. (2004) (73)
Macrophage membrane potential changes associated with gamma 2b/gamma 1 Fc receptor-ligand binding. (1983) (73)
Preparation, characterization, and properties of monoclonal antibodies against the lac carrier protein from Escherichia coli. (1982) (73)
The substrate-binding site in the lactose permease of Escherichia coli. (1998) (72)
Active transport in membrane vesicles from Escherichia coli: the electrochemical proton gradient alters the distribution of the lac carrier between two different kinetic states. (1980) (71)
Fourier transform infrared spectroscopy reveals a rigid alpha-helical assembly for the tetrameric Streptomyces lividans K+ channel. (1998) (71)
Relationship of a membrane-bound D-(-)-lactic dehydrogenase to amino acid transport in isolated bacterial membrane preparations. (1970) (71)
Reconstitution of active transport in proteoliposomes containing cytochrome o oxidase and lac carrier protein purified from Escherichia coli. (1983) (70)
Beta-galactoside transport in bacterial membrane preparations: energy coupling via membrane-bounded D-lactic dehydrogenase. (1970) (70)
Quantitative association between electrical potential across the cytoplasmic membrane and early gentamicin uptake and killing in Staphylococcus aureus (1984) (70)
Energy-dependent binding of dansylgalactosides to the beta-galactoside carrier protein. (1975) (69)
Purified reconstituted lac carrier protein from Escherichia coli is fully functional. (1984) (69)
Monoclonal antibodies against the lac carrier protein from Escherichia coli. 1. Functional studies. (1984) (68)
The Sec-independent Function of Escherichia coli YidC Is Evolutionary-conserved and Essential* (2005) (68)
Immunochemical analysis of membrane vesicles from Escherichia coli. (1979) (68)
Role of proline residues in the structure and function of a membrane transport protein. (1991) (67)
lac permease of Escherichia coli: histidine-205 and histidine-322 play different roles in lactose/H+ symport. (1986) (67)
Expression of lactose permease in contiguous fragments as a probe for membrane-spanning domains. (1994) (66)
Mechanism of lactose translocation in proteoliposomes reconstituted with lac carrier protein purified from Escherichia coli. 2. Deuterium solvent isotope effects. (1983) (66)
Unraveling the mechanism of the lactose permease of Escherichia coli. (2000) (66)
MECHANISM OF LACTOSE TRANSLOCATION IN MEMBRANE VESICLES FROM ESCHERICHIA COLI (1979) (65)
Aromatic stacking in the sugar binding site of the lactose permease. (2003) (65)
Properties of interacting aspartic acid and lysine residues in the lactose permease of Escherichia coli. (1993) (64)
Use of site-directed mutagenesis to study the mechanism of a membrane transport protein. (1987) (64)
Site-directed mutagenesis of cys148 in the lac carrier protein of Escherichia coli. (1984) (64)
Cysteine-scanning mutagenesis of helix IV and the adjoining loops in the lactose permease of Escherichia coli: Glu126 and Arg144 are essential. off. (1997) (64)
Quantification of Detergents Complexed with Membrane Proteins (2017) (64)
Characterization of site-directed mutants in the lac permease of Escherichia coli. 1. Replacement of histidine residues. (1989) (63)
Site-specific mutagenesis of histidine residues in the lac permease of Escherichia coli. (1985) (62)
Identification of the epitope for monoclonal antibody 4B1 which uncouples lactose and proton translocation in the lactose permease of Escherichia coli. (1996) (62)
Site-Directed Mutagenesis and ION-Gradient Driven Active Transport: On the Path of the Proton (1988) (62)
Arg-302 facilitates deprotonation of Glu-325 in the transport mechanism of the lactose permease from Escherichia coli (2001) (61)
Membrane potential in anaerobically growing Staphylococcus aureus and its relationship to gentamicin uptake (1983) (61)
Antigenic architecture of membrane vesicles from Escherichia coli. (1979) (61)
A general method for determining helix packing in membrane proteins in situ: helices I and II are close to helix VII in the lactose permease of Escherichia coli. (1996) (61)
Probing of the rates of alternating access in LacY with Trp fluorescence (2009) (60)
Distance determination in proteins using designed metal ion binding sites and site-directed spin labeling: application to the lactose permease of Escherichia coli. (1995) (60)
Equilibrium between two forms of the lac carrier protein in energized and nonenergized membrane vesicles from Escherichia coli. (1976) (60)
Helix packing in polytopic membrane proteins: the lactose permease of Escherichia coli. (1997) (60)
A molecular mechanism for energy coupling in a membrane transport protein, the lactose permease of Escherichia coli. (1997) (60)
The role of the phosphoenolpyruvate-phosphotransferase system in the transport of sugars by isolated membrane preparations of Escherichia coli. (1968) (60)
Active transport in isolated bacterial membrane vesicles. V. The transport of amino acids by membrane vesicles prepared from Staphylococcus aureus. (1972) (59)
lac permease of Escherichia coli: arginine-302 as a component of the postulated proton relay. (1987) (59)
YidC assists the stepwise and stochastic folding of membrane proteins (2016) (59)
Engineering a metal binding site within a polytopic membrane protein, the lactose permease of Escherichia coli. (1995) (59)
Beta-galactoside transport in E. coli: a functional dissection of lac permease. (1990) (59)
Molecular biology of active transport: from membrane to molecule to mechanism. (1987) (59)
Lactose-proton symport by purified lac carrier protein. (1982) (58)
Effect of tetanus toxin on the accumulation of the permeant lipophilic cation tetraphenylphosphonium by guinea pig brain synaptosomes. (1979) (58)
Functional expression of mouse mdr1 in Escherichia coli. (1993) (58)
Topology of the lac carrier protein in the membrane of Escherichia coli. (1983) (57)
Mutants of Salmonella typhimurium and Escherichia coli pleiotropically defective in active transport. (1972) (57)
Exploiting luminescence spectroscopy to elucidate the interaction between sugar and a tryptophan residue in the lactose permease of Escherichia coli (2003) (56)
SecY alterations that impair membrane protein folding and generate a membrane stress (2007) (56)
Cysteine 148 in the lactose permease of Escherichia coli is a component of a substrate binding site. 1. Site-directed mutagenesis studies. (1994) (56)
Protonation and sugar binding to LacY (2008) (56)
Residues in the H+ translocation site define the pKa for sugar binding to LacY. (2009) (55)
Helix packing of lactose permease in Escherichia coli studied by site-directed chemical cleavage. (1995) (55)
Effects of thyrotropin on the thyroid cell membrane: hyperpolarization induced by hormone-receptor interaction. (1977) (55)
The lactose permease meets Frankenstein. (1994) (54)
Effect of diethylpyrocarbonate on lactose/proton symport in Escherichia coli membrane vesicles. (1979) (54)
Binding affinity of lactose permease is not altered by the H+ electrochemical gradient. (2004) (53)
Proximity between Glu126 and Arg144 in the lactose permease of Escherichia coli. (1999) (53)
Proton-coupled dynamics in lactose permease. (2012) (53)
Proline uptake by disrupted membrane preparations from Escherichia coli. (1969) (53)
cys154 Is important for lac permease activity in Escherichia coli. (1985) (52)
Probing the conformation of the lactose permease of Escherichia coli by in situ site-directed sulfhydryl modification. (1996) (52)
Dynamics of lactose permease of Escherichia coli determined by site-directed fluorescence labeling. (1994) (52)
Mouse macrophage Fc receptor for IgG gamma 2b/gamma 1 in artificial and plasma membrane vesicles functions as a ligand-dependent ionophore. (1983) (52)
Electrophysiological characterization of LacY (2009) (52)
The melibiose/Na+ symporter of Escherichia coli: kinetic and molecular properties. (1990) (52)
Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins (2007) (52)
[74] The use of flow dialysis for determinations of ΔpH and active transport (1979) (52)
Sequence alignment and homology threading reveals prokaryotic and eukaryotic proteins similar to lactose permease. (2006) (52)
Mechanisms of active transport in isolated membrane vesicles. IV. Galactose transport by isolated membrane vesicles from Escherichia coli. (1972) (51)
Cysteine-scanning mutagenesis of putative helix VII in the lactose permease of Escherichia coli. (1993) (51)
Characterization of Glu126 and Arg144, two residues that are indispensable for substrate binding in the lactose permease of Escherichia coli. (1999) (51)
Energetics of Ligand-induced Conformational Flexibility in the Lactose Permease of Escherichia coli* (2006) (51)
Engineering a terbium-binding site into an integral membrane protein for luminescence energy transfer (2002) (51)
Role of glutamate-269 in the lactose permease of Escherichia coli. (1994) (50)
Role of glycine residues in the structure and function of lactose permease, an Escherichia coli membrane transport protein. (1995) (50)
Direct sugar binding to LacY measured by resonance energy transfer. (2006) (50)
Functional and immunochemical characterization of a mutant of Escherichia coli energy uncoupled for lactose transport. (1985) (50)
Correlations between fluorescence, x-ray diffraction, and physiological properties in cytoplasmic membrane vesicles isolated from Escherichia coli. (1972) (49)
Mechanisms of active transport in isolated bacterial membrane vesicles. VII. Fluorescence of 1-anilino-8-naphthalenesulfonate during D-lactate oxidation by membrane vesicles from Escherichia coli. (1972) (49)
Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking (2002) (49)
Fusion proteins as tools for crystallization: the lactose permease from Escherichia coli. (1994) (48)
Effect of Calcium on Intracellular Sodium and Potassium Concentrations in Plant and Animal Cells (1964) (48)
EVALUATION OF THE CHEMIOSMOTIC INTERPRETATION OF ACTIVE TRANSPORT IN BACTERIAL MEMBRANE VESICLES (1974) (47)
Mechanisms of active transport in isolated bacterial membrane vesicles. IX. The kinetics and specificity of amino acid transport in Staphylococcus aureus membrane vesicles. (1972) (47)
Elucidation of substrate binding interactions in a membrane transport protein by mass spectrometry (2003) (47)
Role of cysteine residues in the lac permease of Escherichia coli. (1987) (47)
YidC Protein, a Molecular Chaperone for LacY Protein Folding via the SecYEG Protein Machinery* (2013) (47)
Dansyl-galactoside, a fluorescent probe of active transport in bacterial membrane vesicles. (1973) (46)
Cysteine-scanning mutagenesis of helix II and flanking hydrophilic domains in the lactose permease of Escherichia coli. (1996) (46)
D-lactate dehydrogenase binding in Escherichia coli dld- membrane vesicles reconstituted for active transport. (1974) (46)
Transport in isolated bacterial membrane vesicles. (1974) (46)
Site-directed alkylation of LacY: effect of the proton electrochemical gradient. (2007) (45)
In and out and up and down with lac permease. (1992) (44)
Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: helix VII. (2000) (44)
The central cytoplasmic loop of the major facilitator superfamily of transport proteins governs efficient membrane insertion. (2000) (44)
Structure of LacY with an α-substituted galactoside: Connecting the binding site to the protonation site (2015) (43)
Opening the periplasmic cavity in lactose permease is the limiting step for sugar binding (2011) (43)
Interhelical packing modulates conformational flexibility in the lactose permease of Escherichia coli. (2005) (43)
Helix proximity and ligand-induced conformational changes in the lactose permease of Escherichia coli determined by site-directed chemical crosslinking. (1997) (43)
Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: helices IV and V that contain the major determinants for substrate binding. (2001) (43)
Site-directed mutagenesis of Pro327 in the lac permease of Escherichia coli. (1988) (42)
Trp replacements for tightly interacting Gly–Gly pairs in LacY stabilize an outward-facing conformation (2013) (42)
What to Do While Awaiting Crystals of a Membrane Transport Protein and Thereafter (1999) (42)
D-lactate oxidation and generation of the proton electrochemical gradient in membrane vesicles from Escherichia coli GR19N and in proteoliposomes reconstituted with purified D-lactate dehydrogenase and cytochrome o oxidase. (1986) (42)
IDENTIFICATION OF ANTIGEN 19/27 AS DIHYDROLIPOYL DEHYDROGENASE AND ITS PROBABLE INVOLVEMENT IN UBIQUINONE‐MEDIATED NADH‐DEPENDENT TRANSPORT PHENOMENA IN MEMBRANE VESICLES OF ESCHERICHIA COLI (1980) (41)
The Cys154-->Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway. (2008) (41)
Ligand recognition by the lactose permease of Escherichia coli: specificity and affinity are defined by distinct structural elements of galactopyranosides. (2000) (41)
Direct measurement of lactose/proton symport in Escherichia coli membrane vesicles: further evidence for the involvement of histidine residue(s). (1982) (41)
Use of designed metal-binding sites to study helix proximity in the lactose permease of Escherichia coli. 2. Proximity of helix IX (Arg302) with helix X (His322 and Glu325). (1995) (41)
Proximity of periplasmic loops in the lactose permease of Escherichia coli determined by site-directed cross-linking. (1997) (40)
Functional Overlap but Lack of Complete Cross-Complementation of Streptococcus mutans and Escherichia coli YidC Orthologs (2008) (40)
The role of phosphatidylglycerol in the vectorial phosphorylation of sugar by isolated bacterial membrane preparations. (1970) (40)
Expression of the human erythrocyte glucose transporter in Escherichia coli. (1988) (40)
Apo-intermediate in the transport cycle of lactose permease (LacY) (2012) (40)
Mechanisms of active transport in isolated bacterial membrane vesicles. XII. Active transport by a mutant of Escherichia coli uncoupled for oxidative phosphorylation. (1973) (40)
Mechanism of the melibiose porter in membrane vesicles of Escherichia coli. (1980) (39)
Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: N-ethylmaleimide-sensitive face of helix II. (2000) (39)
What's new with lactose permease (1993) (39)
Transport Studies in Bacterial Membrane Vesicles (1974) (39)
Organization and stability of a polytopic membrane protein: deletion analysis of the lactose permease of Escherichia coli. (1991) (38)
Molecular biology and energetics of membrane transport (1976) (38)
Site-directed spin labeling and chemical crosslinking demonstrate that helix V is close to helices VII and VIII in the lactose permease of Escherichia coli. (1996) (38)
EPR characterization of the iron-sulfur-containing NADH-ubiquinone oxidoreductase of the Escherichia coli aerobic respiratory chain. (1989) (38)
Reversible effects of chaotropic agents on the proton permeability of Escherichia coli membrane vesicles. (1975) (38)
Site-directed spin labeling demonstrates that transmembrane domain XII in the lactose permease of Escherichia coli is an alpha-helix. (1996) (37)
Crystal structure of a LacY–nanobody complex in a periplasmic-open conformation (2016) (37)
The role of helix VIII in the lactose permease of Escherichia coli: II. Site‐directed sulfhydryl modification (1997) (37)
Effect of the proton electrochemical gradient on maleimide inactivation of active transport in Escherichia coli membrane vesicles. (1981) (37)
In Vitro Synthesis of Lactose Permease to Probe the Mechanism of Membrane Insertion and Folding* (2003) (37)
Binding of enzyme IIAGlc, a component of the phosphoenolpyruvate:sugar phosphotransferase system, to the Escherichia coli lactose permease. (2002) (37)
Insertional mutagenesis of hydrophilic domains in the lactose permease of Escherichia coli. (1992) (37)
Delineating Electrogenic Reactions during Lactose/H+ Symport† (2010) (37)
Site-directed mutagenesis of cysteine-148 in the lac permease of Escherichia coli: effect on transport, binding, and sulfhydryl inactivation. (1985) (37)
Mechanisms of active transport in isolated bacterial membrane vesicles. X. Inactivation of D-lactate dehydrogenase and D-lactate dehydrogenase-coupled transport in Escherichia coli membrane vesicles by an acetylenic substrate. (1972) (37)
Photoinactivation of the beta-galactoside transport system in Escherichia coli membrane vesicles with 2-nitro-4-azidophenyl-1-thio-beta-D-galactopyranoside. (1975) (36)
Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane (2010) (36)
Thiol cross-linking of transmembrane domains IV and V in the lactose permease of Escherichia coli. (2000) (36)
Design of a membrane transport protein for fluorescence spectroscopy. (1990) (36)
Use of designed metal-binding sites to study helix proximity in the lactose permease of Escherichia coli. 1. Proximity of helix VII (Asp237 and Asp240) with helices X (Lys319) and XI (Lys358). (1995) (36)
Kinetic analysis of lactose exchange in proteoliposomes reconstituted with purified lac permease. (1991) (36)
Intermolecular thiol cross-linking via loops in the lactose permease of Escherichia coli (2003) (35)
The lac permease of Escherichia coli: site-directed mutagenesis studies on the mechanism of beta-galactoside/H+ symport. (1990) (34)
Glycine uptake in Escherichia coli. II. Glycine uptake, exchange, and metabolism by an isolated membrane preparation. (1968) (34)
Active transport in membrane vesicles from Escherichia coli: the electrochemical proton gradient alters the distribution of the lac carrier between two different kinetic states. (1980) (34)
Evolutionary mix-and-match with MFS transporters II (2013) (34)
Relationship between the Na+/H+ antiporter and Na+/substrate symport in Bacillus alcalophilus. (1981) (34)
Mechanisms of active transport in isolated bacterial membrane vesicles. Further studies on amino acid transport in Staphylococcus aureus membrane vesicles. (1972) (34)
Interaction between residues Glu269 (helix VIII) and His322 (helix X) of the lactose permease of Escherichia coli is essential for substrate binding. (1997) (34)
The lactose permease of : a paradigm for membrane transport proteins (1992) (34)
The role of functional sulfhydryl groups in active transport in Escherichia coli membrane vesicles. (1978) (34)
The C-4 hydroxyl group of galactopyranosides is the major determinant for ligand recognition by the lactose permease of Escherichia coli. (2001) (33)
Substrate-induced changes in the structural properties of LacY (2014) (33)
Site-directed spin-labeling of transmembrane domain VII and the 4B1 antibody epitope in the lactose permease of Escherichia coli. (1997) (33)
Cysteine scanning mutagenesis of the N‐terminal 32 amino acid residues in the lactose permease of Escherichia coli (1994) (33)
Glucose 6-phosphate transport in membrane vesicles isolated from Escherichia coli: effect of imposed electrical potential and pH gradient. (1980) (32)
Conservation of residues involved in sugar/H(+) symport by the sucrose permease of Escherichia coli relative to lactose permease. (2006) (32)
Cysteine scanning mutagenesis of putative helix XI in the lactose permease of Escherichia coli. (1993) (32)
A five-residue sequence near the carboxyl terminus of the polytopic membrane protein lac permease is required for stability within the membrane. (1989) (32)
Ligand‐Induced conformational changes in the lactose permease of escherichia coli: Evidence for two binding sites (1994) (32)
Role of protons in sugar binding to LacY (2012) (32)
Effect of distance and orientation between arginine-302, histidine-322, and glutamate-325 on the activity of lac permease from Escherichia coli. (1989) (32)
Engineering conformational flexibility in the lactose permease of Escherichia coli: use of glycine-scanning mutagenesis to rescue mutant Glu325-->Asp. (2001) (32)
Carriers for nucleotide 5’-triphosphates. Liquid membrane and liposome transport (1993) (32)
Sequential truncation of the lactose permease over a three-amino acid sequence near the carboxyl terminus leads to progressive loss of activity and stability. (1991) (32)
Evidence for an intermediate conformational state of LacY (2012) (31)
Information content of amino acid residues in putative helix VIII of the lac permease from Escherichia coli. (1990) (31)
Exchange, efflux, and substrate binding by cysteine mutants of the lactose permease of Escherichia coli. (1993) (31)
Sugar Recognition by the Lactose Permease of Escherichia coli* (2004) (31)
Alanine Insertion Scanning Mutagenesis of Lactose Permease Transmembrane Helices* (1997) (31)
Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport. (2011) (31)
Cysteine-scanning mutagenesis of transmembrane domain XII and the flanking periplasmic loop in the lactose permease of EScherichia coli. (1996) (30)
It takes two to tango: The dance of the permease (2019) (30)
Trypsin proteolysis of the cytochrome d complex of Escherichia coli selectively inhibits ubiquinol oxidase activity while not affecting N,N,N',N'-tetramethyl-p-phenylenediamine oxidase activity. (1988) (30)
Photoinactivation of the beta-galactoside transport system in Escherichia coli membrane vesicles with an impermeant azidophenylgalactoside. (1975) (30)
Morphology of proteoliposomes reconstituted with purified lac carrier protein from Escherichia coli. (1984) (30)
lac permease of Escherichia coli containing a single histidine residue is fully functional. (1988) (30)
Molecular Aspects of Membrane Phenomena (1975) (30)
Monoclonal antibody 4B1 alters the pKa of a carboxylic acid at position 325 (helix X) of the lactose permease of Escherichia coli. (1996) (30)
Dynamics of lactose permease of Escherichia coli determined by site-directed chemical labeling and fluorescence spectroscopy. (1995) (29)
The role of the carbodiimide-reactive component of the adenosine-5'-triphosphatase complex in the proton permeability of Escherichia coli membrane vesicles. (1976) (29)
Chapter 10 The lactose permease of Escherichia coli: Past, present and future (1996) (29)
Transmembrane helix tilting and ligand-induced conformational changes in the lactose permease determined by site-directed chemical crosslinking in situ. (1998) (29)
Mechanism of lactose transport in Escherichia coli membrane vesicles: evidence for the involvement of histidine residue(s) in the response of the lac carrier to the proton electrochemical gradient. (1982) (29)
Binding of hydrophobic D-galactopyranosides to the lactose permease of Escherichia coli. (2002) (29)
Lifetime and rotational relaxation time of dansylgalactoside bound to the lac carrier protein. (1975) (29)
Engineering the lac permease for purification and crystallization (1996) (29)
Studies on the metabolism of ATP by isolated bacterial membranes: formation and metabolism of membrane-bound phosphatidic acid. (1971) (29)
The role of helix VIII in the lactose permease of Escherichia coli: I. Cys‐scanning mutagenesis (1997) (29)
Observing a lipid-dependent alteration in single lactose permeases. (2015) (29)
Specific labeling of the lac carrier protein in membrane vesicles of Escherichia coli by a photoaffinity reagent. (1980) (28)
[17] Oligonucleotide-directed site-specific mutagenesis of the 1ac permease of Escherichia coli (1986) (28)
The lactose permease of Escherichia coli: a paradigm for membrane transport proteins. (1992) (28)
Ubiquinone-mediated coupling of NADH dehydrogenase to active transport in membrane vesicles from Escherichia coli. (1975) (28)
The last two cytoplasmic loops in the lactose permease of Escherichia coli comprise a discontinuous epitope for a monoclonal antibody. (1997) (28)
Changing the lactose permease of Escherichia coli into a galactose-specific symporter (2002) (28)
The lac carrier protein from Escherichia coli. (1984) (28)
The Transport of Sugars Across Isolated Bacterial Membranes (1971) (28)
Helix packing in the lactose permease determined by metal-nitroxide interaction. (1998) (27)
Sodium‐dependent glutamate transport in membrane vesicles of Escherichia coli K‐12 (1975) (27)
Crystal Structure of a ligand-bound LacY–Nanobody Complex (2018) (27)
Conformations of the rhodopsin third cytoplasmic loop grafted onto bacteriorhodopsin. (2000) (27)
Electrochemical proton gradient in Micrococcus lysodeikticus cells and membrane vesicles (1980) (27)
Helix packing in the sucrose permease of Escherichia coli: properties of engineered charge pairs between helices VII and XI. (1995) (27)
Differentiation between binding and transport of dansylgalactosides in Escherichia coli. (1975) (27)
Thiol cross-linking of cytoplasmic loops in the lactose permease of Escherichia coli. (2000) (27)
Site-directed mutagenesis of tyrosine residues in the lac permease of Escherichia coli. (1989) (27)
Resolution and identification of iron-containing antigens in membrane vesicles from Escherichia coli. (1980) (26)
Exploring the Role of a Unique Carboxyl Residue in EmrE by Mass Spectrometry* (2005) (26)
Active transport by the CscB permease in Escherichia coli K-12. (1995) (26)
Topology of allosteric regulation of lactose permease. (1997) (26)
Membrane topology of helices VII and XI in the lactose permease of Escherichia coli studied by lacY-phoA fusion analysis and site-directed spectroscopy. (1995) (26)
The sucrose permease of Escherichia coli: functional significance of cysteine residues and properties of a cysteine-less transporter. (2000) (26)
Insertion and folding pathways of single membrane proteins guided by translocases and insertases (2019) (26)
Deduction of consensus binding sequences on proteins that bind IIAGlc of the phosphoenolpyruvate:sugar phosphotransferase system by cysteine scanning mutagenesis of Escherichia coli lactose permease. (1999) (26)
Insertion of the polytopic membrane protein lactose permease occurs by multiple mechanisms. (1995) (25)
Characterization of purified, reconstituted site-directed cysteine mutants of the lactose permease of Escherichia coli. (1991) (25)
Design of a membrane protein for site-specific proteolysis: properties of engineered factor Xa protease sites in the lactose permease of Escherichia coli. (1995) (25)
Helix dynamics in LacY: helices II and IV. (2010) (25)
Functional complementation of internal deletion mutants in the lactose permease of Escherichia coli. (1992) (25)
Helix packing in the lactose permease of Escherichia coli: distances between site-directed nitroxides and a lanthanide. (2001) (25)
Reconstitution of D-Lactate-Dependent Transport in Membrane Vesicles from a D-Lactate Dehydrogenase Mutant of Escherichia coli (1973) (25)
The N-terminal 22 amino acid residues in the lactose permease of Escherichia coli are not obligatory for membrane insertion or transport activity. (1992) (25)
Site-directed alkylation of cysteine replacements in the lactose permease of Escherichia coli: helices I, III, VI, and XI. (2006) (24)
Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: helix X. (2000) (24)
Proximity between periplasmic loops in the lactose permease of Escherichia coli as determined by site-directed spin labeling. (1999) (24)
Active transport in Escherichia coli: passage to permease. (1986) (24)
Glycine uptake in Escherichia coli. I. Glycine uptake by whole cells of Escherichia coli W+ and a D-serine-resistant. (1968) (24)
Site-specific mutagenesis of cysteine 148 to serine in the lac permease of Escherichia coli. (1986) (24)
Regulation of sugar transport in isolated bacterial membrane preparations from Escherichia coli. (1969) (23)
Tilting of helix I and ligand-induced changes in the lactose permease determined by site-directed chemical cross-linking in situ. (1998) (23)
Proton Electrochemical Gradients and Active Transport: The Saga of lac Permease (1985) (23)
Functional molecular weight of the lac carrier protein from Escherichia coli as studied by radiation inactivation analysis. (1984) (23)
TheLac carrier protein inEscherichia coli (2005) (23)
Fluorescent galactosides as probes for the lac carrier protein. (1977) (23)
Site-directed chemical cross-linking demonstrates that helix IV is close to helices VII and XI in the lactose permease. (1999) (23)
Helices VII and X in the lactose permease of Escherichia coli: proximity and ligand-induced distance changes. (2002) (22)
In vitro synthesis of the membrane-bound D-lactate dehydrogenase of Escherichia coli. (1982) (22)
pKa of Glu325 in LacY (2017) (22)
Nitroxide scanning electron paramagnetic resonance of helices IV and V and the intervening loop in the lactose permease of Escherichia coli. (1999) (22)
Outward-facing conformers of LacY stabilized by nanobodies (2014) (22)
Characterization and functional reconstitution of a soluble form of the hydrophobic membrane protein lac permease from Escherichia coli. (1989) (22)
Residues gating the periplasmic pathway of LacY. (2009) (22)
Reconstitution of ubiquinone-linked functions in membrane vesicles from a double quinone mutant of Escherichia coli. (1979) (22)
Fluorescence of native single‐Trp mutants in the lactose permease from Escherichia coli: Structural properties and evidence for a substrate‐induced conformational change (1995) (22)
Manipulating conformational equilibria in the lactose permease of Escherichia coli. (2002) (22)
Dynamics of the l-fucose/H+ symporter revealed by fluorescence spectroscopy (2012) (22)
Monitoring Conformational Rearrangements in the Substrate-binding Site of a Membrane Transport Protein by Mass Spectrometry* (2004) (21)
A conformational change in the lactose permease of Escherichia coli is induced by ligand binding or membrane potential (1994) (21)
Site-directed mutagenesis and ion-gradient driven active transport: on the path of the proton. (1988) (21)
Determination of the absolute number of Escherichia coli membrane vesicles that catalyze active transport. (1974) (21)
Estimating Loop−Helix Interfaces in a Polytopic Membrane Protein by Deletion Analysis† (1999) (21)
The lac carrier protein in Escherichia coli. (1983) (21)
Binding of ligand or monoclonal antibody 4B1 induces discrete structural changes in the lactose permease of Escherichia coli. (1997) (20)
Sulfhydryl oxidation of mutants with cysteine in place of acidic residues in the lactose permease. (1998) (20)
Transient conformers of LacY are trapped by nanobodies (2015) (20)
Cysteine 148 in the lactose permease of Escherichia coli is a component of a substrate binding site. 2. Site-directed fluorescence studies. (1994) (20)
Evidence that the final turn of the last transmembrane helix in the lactose permease is required for folding. (1992) (20)
Real-time conformational changes in LacY (2014) (19)
Vinylglycolic acid. An inactivator of the phosphoenolpyruvate-phosphate transferase system in Escherichia coli. (1973) (19)
Thermodynamic mechanism for inhibition of lactose permease by the phosphotransferase protein IIAGlc (2015) (19)
Solubilization and Partial Purification of Amino Acid-Specific Components of the D-Lactate Dehyrogenase-Coupled Amino Acid-Transport Systems (1972) (19)
ATP-driven active transport in right-side-out bacterial membrane vesicles. (1981) (19)
Involvement of the proton electrochemical gradient in genetic transformation in Escherichia coli. (1981) (19)
Energy-dependent binding of dansylgalactoside to the lac carrier protein: direct binding measurements. (1976) (19)
CHAPTER 6 – Active Transport: Membrane Vesicles, Bioenergetics, Molecules, and Mechanisms (1990) (19)
Sodium-dependent binding of p-nitrophenyl alpha-D-galactopyranoside to membrane vesicles isolated from Salmonella typhimurium. (1978) (19)
Lac permease of Escherichia coli: on the path of the proton. (1990) (18)
Role of the irreplaceable residues in the LacY alternating access mechanism (2012) (18)
Purification and reconstitution of the cytochrome o-type oxidase from Escherichia coli. (1986) (18)
Clogging the periplasmic pathway in LacY. (2009) (18)
Conformational flexibility at the substrate binding site in the lactose permease of Escherichia coli. (1999) (18)
Immunochemical properties of the membrane-bound D-lactate dehydrogenase from Escherichia coli. (1975) (18)
Molecular biology of active transport: from membrane to molecule to mechanism. (1987) (18)
Cysteine scanning mutagenesis of helix V in the lactose permease of Escherichia coli. (1995) (18)
An Early Event in the Transport Mechanism of LacY Protein (2011) (18)
Purification and properties of D-lactate dehydrogenase from Escherichia coli ML 308-225. (1978) (18)
MEMBRANE TRANSPORT AS A POTENTIAL TARGET FOR ANTIBIOTIC ACTION (1974) (18)
Chemical rescue of Asp237-->Ala and Lys358-->Ala mutants in the lactose permease of Escherichia coli. (1996) (18)
Site-directed sulfhydryl labeling of helix IX in the lactose permease of Escherichia coli. (2003) (17)
Physiological suppression of a transport defect in Escherichia coli mutants deficient in Ca2+, Mg2+-stimulated adenosine triphosphatase (1975) (17)
The role of transmembrane domain III in the lactose permease of escherichia coli (1994) (17)
2007 Annual progress report synopsis of the Center for Structures of Membrane Proteins (2009) (17)
Helix packing in the lactose permease of Escherichia coli determined by site-directed thiol cross-linking: helix I is close to helices V and XI. (1999) (17)
pH Regulation of Electrogenic Sugar/H+ Symport in MFS Sugar Permeases (2016) (17)
Mechanism of β-galactoside transport in Escherichia coli membrane vesicles (1976) (17)
Arginine 302 (helix IX) in the lactose permease of Escherichia coli is in close proximity to glutamate 269 (helix VIII) as well as glutamate 325. (1997) (17)
Thiol cross-linking of transmembrane domains IV and V in the lactose permease of Escherichia coli. (2000) (17)
The use of flow dialysis for determinations of deltapH and active transport. (1979) (16)
Effect of the lipid phase transition on the lactose permease from Escherichia coli. (2000) (16)
Tertiary contacts of helix V in the lactose permease determined by site-directed chemical cross-linking in situ. (1999) (16)
ENERGETICS AND MECHANISM OF LACTOSE TRANSLOCATION IN ISOLATED MEMBRANE VESICLES OF ESCHERICHIA COLI (1980) (16)
Proximity of helices VIII (Ala273) and IX (Met299) in the lactose permease of Escherichia coli. (1998) (15)
Reconstitution of Escherichia coli membrane vesicles with D-amino acid dehydrogenase. (1981) (15)
Electrophysiological characterization of uncoupled mutants of LacY. (2013) (15)
Location of helix III in the lactose permease of Escherichia coli as determined by site-directed thiol cross-linking. (1999) (15)
In vitro biotinylation provides quantitative recovery of highly purified active lactose permease in a single step. (1998) (15)
Active Transport in Isolated Bacterial Membrane Vesicles (1974) (15)
Interaction between residues Glu269 (helix VIII) and His322 (helix X) of the lactose permease of Escherichia coli is essential for substrate binding. (1997) (15)
[33] Preparation of monoclonal antibodies and site-directed polyclonal antibodies against the lac permease of Escherichia coli (1986) (15)
Helix packing in the lactose permease of Escherichia coli: localization of helix VI. (2001) (15)
Ligand-induced movement of helix X in the lactose permease from Escherichia coli: a fluorescence quenching study. (1997) (14)
Regulation of sugar transport in isolated bacterial membrane preparations from Escherichia coli. (1969) (14)
Energetics and molecular biology of active transport in bacterial membrane vesicles. (1977) (14)
Vinylglycolate resistance in Escherichia coli (1975) (14)
Integrating mass spectrometry into membrane protein drug discovery. (2004) (14)
Sugar recognition by CscB and LacY. (2011) (14)
Synthesis of cyclopropane fatty acids in isolated bacterial membranes. (1973) (14)
Functional estimation of loop-helix boundaries in the lactose permease of Escherichia coli by single amino acid deletion analysis. (2001) (13)
Association between the amino- and carboxyl-terminal halves of lactose permease is specific and mediated by multiple transmembrane domains. (1996) (13)
Histidine-94 is the only important histidine residue in the melibiose permease of Escherichia coli. (1990) (13)
Functional conservation in the putative substrate binding site of the sucrose permease from Escherichia coli. (2000) (13)
In vitro folding of a membrane protein: Effect of denaturation and renaturation on substrate binding by the lactose permease of Escherichia coli (1998) (13)
Galactoside-Binding Site in LacY (2014) (13)
Purification and functional characterization of the C-terminal half of the lactose permease of Escherichia coli. (1996) (13)
Ligand-induced changes in periplasmic loops in the lactose permease of Escherichia coli. (1998) (13)
Factors influencing the accumulation of tetraphenylphosphonium cation in HeLa cells (1984) (13)
Binding of monoclonal antibody 4B1 to homologs of the lactose permease of Escherichia coli (1997) (13)
Further studies on metabolism of phosphatidic acid of isolated E. coli membrane vesicles. (1972) (13)
Asymmetric binding of membrane proteins to GroEL. (2005) (13)
Simultaneous reconstitution of Escherichia coli membrane vesicles with D-lactate and D-amino acid dehydrogenases. (1982) (12)
Proximity relationships between helices I and XI or XII in the lactose permease of Escherichia coli determined by site-directed thiol cross-linking. (1999) (12)
Site-directed mutagenesis of lysine 319 in the lactose permease of Escherichia coli. (1992) (12)
Cysteine-scanning mutagenesis of helix VI and the flanking hydrophilic domains on the lactose permease of Escherichia coli. (1996) (12)
Use of site-directed mutagenesis to study the mechanism of a membrane transport protein. (1987) (12)
Direct measurement of the electrogenic activity of o-type cytochrome oxidase from Escherichia coli reconstituted into planar lipid bilayers. (1985) (12)
Effect of lipids on the reconstitution of D-lactate oxidase in Escherichia coli membrane vesicles. (1976) (12)
Melibiose permease and alpha-galactosidase of Escherichia coli: identification by selective labeling using a T7 RNA polymerase/promoter expression system. (1990) (12)
Probing the Mechanism of a Membrane Transport Protein with Affinity Inactivators* (2003) (11)
The Life and Times of Lac Permease: Crystals Ain’t Everything, but They Certainly Do Help (2014) (11)
Transport in an Escherichia coli fatty acid auxotroph. A novel case of catabolite repression. (1975) (11)
Reconstitution of LAC carrier function in cholate-extracted membranes from Escherichia coli. (1979) (11)
Arg302 governs the pKa of Glu325 in LacY (2019) (11)
Properties of a LacY efflux mutant. (2009) (10)
Estimating loop-helix interfaces in a polytopic membrane protein by deletion analysis. (1999) (10)
Chapter 22 Membrane Vesicles, Electrochemical Ion Gradients, and Active Transport (1982) (10)
Relationship between amino acid transport and electron transport by membrane vesicles of Micrococcus denitrificans. (1974) (10)
Electrochemical ion gradients and active transport. (1980) (10)
Melibiose permease of Escherichia coli: mutation of histidine-94 alters expression and stability rather than catalytic activity. (1992) (10)
The lac permease of Escherichia coli: a prototypic energy-transducing membrane protein. (1990) (10)
Microenvironment of the binding site in the lac carrier protein. (1977) (10)
A method for rapid, continuous monitoring of solute uptake and binding. (1982) (9)
Reversible inactivation of vectorial phosphorylation by hydroxybutynoate in Escherichia coli membrane vesicles. (1975) (9)
Permease on parade: Application of site‐directed mutagenesis to ion‐gradient driven active transport (1987) (9)
Defective transport in S-adenosylmethionine synthetase mutants of Escherichia coli. (1974) (9)
The periplasmic cavity of LacY mutant Cys154→Gly: how open is open? (2013) (9)
Lac permease of Escherichia coli: on the path of the proton. (1990) (9)
Active Transport in Escherichia Coli From Membrane to Molecule (1986) (9)
Preface to volume 2 Transport processes in eukaryotic and prokaryotic organisms (1996) (9)
Glucose/Sugar Transport in Bacteria (2004) (9)
Structure/function studies on the lactose permease of Escherichia coli. (1998) (8)
Ligand-induced differences in secondary structure of the Vibrio parahaemolyticus Na+/galactose cotransporter. (2002) (8)
Information content of amino acid residues in putative helix VIII of the lac permease from Escherichia coli. (1990) (8)
Characterization of a lactose permease mutant that binds IIAGlc in the absence of ligand. (2003) (8)
An Asymmetric Conformational Change in LacY. (2017) (8)
Isolation and functional reconstitution of soluble melibiose permease from Escherichia coli. (1990) (8)
Nucleotide binding by Escherichia coli membranes and solubilized membrane proteins. (1969) (8)
The proton electrochemical gradient induces a kinetic asymmetry in the symport cycle of LacY (2019) (7)
Substitution of glutamine-60 with glutamic acid causes the lac permease of Escherichia coli to become temperature sensitive. (1986) (7)
β-Galactoside Transport in Escherichia Coli: The Ins and Outs of Lactose Permease (1992) (7)
Studies on the metabolism of ATP by isolated bacterial membranes: solubilization and phosphorylation of a protein component of the diglyceride kinase system. (1973) (7)
Engineering the lac permease for purification and crystallization (1996) (7)
Isolation and functional reconstitution of soluble melibiose permease from Escherichia coli. (1990) (6)
It's Better To Be Lucky Than Smart. (2021) (6)
The Lactose Permease of Escherichia coli An Update (1996) (6)
Oversized galactosides as a probe for conformational dynamics in LacY (2018) (6)
Thermodynamics of Nanobody Binding to Lactose Permease. (2016) (5)
Monoclonal antibodies against the membrane-bound, flavin-linked D-lactate dehydrogenase of Escherichia coli: preparation, characterization, and use in immunoaffinity chromatography. (1985) (5)
Role of Conserved Gly-Gly Pairs on the Periplasmic Side of LacY. (2016) (5)
Topology of the lac Permease Protein in the Membrane of Escherichia coli (1985) (5)
Membranes: A new era in membrane protein studies (2004) (5)
Handbook of Biological Physics (1996) (5)
The electrochemical proton gradient in Escherichia coli membrane vesicles and its relationship to active transport. (1977) (4)
Reconstitution of D-lactate-dependent transport in membrane vesicles from a D-lactate dehydrogenase mutant of Escherichia coli. (1973) (4)
Engineered occluded apo-intermediate of LacY (2018) (4)
The lac permease of Escherichia coli: a prototypic energy-transducing membrane protein. (1990) (4)
Helix packing in the C-terminal half of lactose permease (1995) (4)
Proton Electrochemical Gradients and Active Transport: The Saga of lac Permease (1985) (4)
Functional estimation of loop-helix boundaries in the lactose permease of Escherichia coli by single amino acid deletion analysis. (2001) (4)
ELECTROCHEMICAL ION GRADIENTS AND ACTIVE TRANSPORT (1980) (4)
[35] Monoclonal affinity purification of d-lactate dehydrogenase from Escherichia coli (1986) (3)
ACTIVE TRANSPORT IN ISOLATED BACTERIAL MEMBRANE VESICLES: BINDING OF β‐GALACTOSIDES TO THE LAC CARRIER PROTEIN (1975) (3)
Monoclonal affinity purification of D-lactate dehydrogenase from Escherichia coli. (1986) (3)
Structure/function studies on the lactose permease of Escherichia coli. (1998) (3)
Binding of spin-labeled galactosides to the lactose permease of Escherichia coli. (2000) (3)
Active transport in bacterial cytoplasmic membrane vesicles. (1973) (3)
TRANSPORT MECHANISMS IN ISOLATED BACTERIAL CYTOPLASMIC MEMBRANE VESICLES (1972) (2)
Site-directed mutagenesis of the lacY gene of Escherichia coli. (1985) (2)
Active transport in bacterial cytoplasmic membrane vesicles. (1973) (2)
The lactose permease of Escherichia coli: a paradigm for membrane transport proteins. (1993) (2)
In vitro folding of a membrane protein: effect of denaturation and renaturation on substrate binding by the lactose permease of Escherichia coli. (1998) (2)
Single-molecule FRET reveals sugar-induced conformational dynamics (2008) (2)
Investigation of sugar binding kinetics of the E. coli sugar/H+ symporter XylE using solid-supported membrane-based electrophysiology (2021) (2)
Crystal structure of lactose permease (2003) (2)
Preparation of monoclonal antibodies and site-directed polyclonal antibodies against the lac permease of Escherichia coli. (1986) (2)
Molecular aspects of membrane phenomena: International symposium held at the Battelle Seattle Research Center, Seattle, WA, USA, November 4-6, 1974 : [papers] (1975) (2)
Diversity in kinetics correlated with structure in nano body-stabilized LacY (2020) (2)
Mechanism of the melibiose porter in membrane vesicles of Escherichia coli. (1980) (2)
MOLECULAR ASPECTS OF ACTIVE TRANSPORT (1976) (1)
The lac permease of Escherichia coli: a prototype transport protein (1990) (1)
Discussion paper: bacterial transport mechanisms as studied in cytoplasmic membrane vesicles. (1972) (1)
Site‐directed Mutagenesis of the lac Y Gene of Escherichia coli (1985) (1)
Effect of the proton electrochemical gradient on maleimide inactivation of active transport in Escherichia coli membrane vesicles. (1981) (1)
7. Energetics and Molecular Biology of Membrane Transport (1978) (1)
Molecular biology of receptors and transporters. Bacterial and glucose transporters. Introduction. (1992) (1)
Solubilization and partial purification of amino acid-specific components of the D-lactate dehydrogenase-coupled amino acid-transport systems (E. coli-cell membranes-sephadex-detergent-solubilized-vesicles). (1972) (1)
Visualization of Membrane Proteins in GroEL (2004) (1)
Sugar and amino acid transport mechanisms in isolated bacterial membrane vesicles (1971) (1)
Oligonucleotide-directed site-specific mutagenesis of the lac permease of Escherichia coli. (1986) (1)
DISCUSSION PAPER: BACTERIAL TRANSPORT MECHANISMS AS STUDIED IN CYTOPLASMIC MEMBRANE VESICLES (1972) (1)
Kinetics of electron transfer in reaction center‐cytochrome o proteoliposomes (1986) (1)
Isolated Bacterial Cytoplasmic Membrane Vesicles: A Model System for the Study of Active Transport (1975) (0)
Title Role of Conserved Gly-Gly Pairs on the Periplasmic Side of LacY Permalink (2016) (0)
CORRECTION (2005) (0)
Sugar Free Lactose Permease at acidic pH (2006) (0)
Molecular biology of receptors and transporters. Bacterial and glucose transporters. Introduction. (1992) (0)
D-Lactate Dehydrogenase Binding inEscherichia colidid-Membrane Vesicles Reconstituted forActive Transport* (guanidine *HCI/hydroxybutynoate/vinylglycolate/dansyl-galactoside) (1974) (0)
revealed by comparative modeling Structural conservation in the major facilitator superfamily as (2004) (0)
Helix dynamics in LacY: helices II/IV (2011) (0)
crystal structure of a LacY/Nanobody complex (2016) (0)
Carriers for Nucleotide 5′-Triphosphates. Part 2. Liquid Membrane and Liposome Transport. (1993) (0)
Relationship ofaMembrane-Bound D-(-)-Lactic Dehydrogenase toAminoAcidTransport inIsolated Bacterial MembranePreparations (1970) (0)
University of Groningen Characterization of Site-Directed Mutants in the lac Permease of Escherichia coli . 2 . Glutamate-325 (2001) (0)
Kinetic Effects of Inside‐Positive Membrane Potential on the Lactose Efflux of LacY (2020) (0)
Lactose Permease: From Membrane to Molecule to Mechanism (2019) (0)
Helix packing in the lactose permease of escherichia coti as determined by use of designed metal ion binding sites and site-directed gpin labeling (1996) (0)
In Vivo and In Vitro Characterizations of Nanobodies Raised Against the Melibiose Permease melB of Salmonella Typhimurium (2021) (0)
MOLECULAR BIOLOGY AND ENERGETICS OF ACTIVE TRANSPORT (1978) (0)
Thermodynamic Insights into Conformational Dynamics of Sugar Transporters (2016) (0)
Determination oftheAbsolute NumberofEscherwhia coliMembrane Vesicles ThatCatalyze Active Transport*t (1974) (0)
In Vivo and in Vitro Characterizations of Conformational Nanobodies of Salmonella Enterica Serovar Typhimurium Melibiose Permease Melb (2023) (0)
Crystal structure of E. coli Lactose permease G46W/G262W bound to p-nitrophenyl alpha-D-galactopyranoside (alpha-NPG) (2015) (0)
All about lac permease (2006) (0)
The Lactose Permease of Escherichia coli: The Kamikaze Approach to Membrane Protein Structure and Function (2000) (0)
Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY (2013) (0)
Mass Spectrometry of Membrane Transport Proteins (2003) (0)
Sugar Free Lactose Permease at neutral pH (2006) (0)
Biochemistry lac permease of Escherichia coli containing a single histidine residue is fully functional ( lacY gene product / active transport / lactose / H + symport / electrochemnical proton gradient / site-directed mutagenesis (0)
, Escherichia coliof Structure and Mechanism of the Lactose Permease (2008) (0)
From membrane to molecule with the lac permease of Escherichia coli. (1985) (0)
Site-Directed Mutagenesis of Pro 327 in the lac Permease of Escherichia coli (2001) (0)
Lactose permease complex with thiodigalactoside and nanobody 9043 (2020) (0)
Monoclonal antibody 4B1 influences the pKa of Glu325 in lactose permease (LacY) from Escherichia coli: evidence from SEIRAS (2020) (0)
Discussion paper: bacterial transport mechanisms as studied in cytoplasmic membrane vesicles. (1972) (0)
Studies of a Biological Energy Transducer (1986) (0)
Crystal structure of E. coli lactose permease G46W,G262W bound to sugar (2014) (0)
Crystal structure of LacY in complex with an affinity inactivator (2011) (0)
Solute binding and transformation during transport. (1970) (0)
University of Groningen Site-Directed Mutagenesis of Pro 327 in the lac Permease of Escherichia coli (2017) (0)
Structural Analysis of Murine Voltage Dependent Anion Channel (VDAC) 1 (2006) (0)
Wild-type Structure of Lactose Permease (2007) (0)
The old Man and the Membrane (2011) (0)
Corrections. (2020) (0)
TheLac carrier protein inEscherichia coli (1983) (0)
Cell and molecular physiology. Modulation of membrane transport systems (1988) (0)
Escherichia coli: 11. Site-directed sulfhydryl modification (2002) (0)
Obituary: Wilhelmus Nicolaas Konings (1937-2014) (2014) (0)
Crystal structure of lactose permease with TDG (2003) (0)
Lactose Permease: Mechanism through Structures (2018) (0)
P1Lactose permease: A beautiful chemiosmotic machine (2012) (0)
Functional molecular weight of the Escherichia coli as studied by radia (target theory/proton electrochemical gradient/symport/oligomerizal (2016) (0)
Solute binding and transformation during transport. (1970) (0)

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