Ulrich Schwaneberg
German chemist
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Chemistry
Ulrich Schwaneberg's Degrees
- PhD Chemistry RWTH Aachen University
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(Suggest an Edit or Addition)According to Wikipedia, Ulrich Schwaneberg is a German chemist and protein engineer. He is the Chair of Biotechnology at RWTH Aachen University and member of the scientific board at the Leibniz Institute for Interactive Materials in Aachen. He specializes in directed evolution of proteins for material science applications and on the development of its methodologies. The latter comprise methods for diversity generation , as well as high-throughput screening systems . His work group has elucidated general design principles of enzymes by analyzing libraries that contain the full natural diversity of a hydrolase with single amino acid exchanges and developed strategies to efficiently explore the protein sequence space and discovered protein engineering principles.
Ulrich Schwaneberg's Published Works
Published Works
- Laboratory evolution of cytochrome P450 BM‐3 monooxygenase for organic cosolvents (2004) (186)
- Advances in generating functional diversity for directed protein evolution. (2009) (167)
- Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution. (2004) (166)
- Directed evolution of the fatty-acid hydroxylase P450 BM-3 into an indole-hydroxylating catalyst. (2000) (164)
- A statistical analysis of random mutagenesis methods used for directed protein evolution. (2006) (147)
- A continuous spectrophotometric assay for P450 BM-3, a fatty acid hydroxylating enzyme, and its mutant F87A. (1999) (146)
- A P450 BM-3 mutant hydroxylates alkanes, cycloalkanes, arenes and heteroarenes. (2001) (139)
- Directed Evolution of a Cytochrome P450 Monooxygenase for Alkane Oxidation (2001) (135)
- Directed Evolution Empowered Redesign of Natural Proteins for the Sustainable Production of Chemicals and Pharmaceuticals. (2018) (128)
- Making glucose oxidase fit for biofuel cell applications by directed protein evolution. (2006) (128)
- Applying metagenomics for the identification of bacterial cellulases that are stable in ionic liquids (2009) (126)
- The diversity challenge in directed protein evolution. (2006) (120)
- Protein engineering in bioelectrocatalysis. (2003) (118)
- Reengineering CelA2 cellulase for hydrolysis in aqueous solutions of deep eutectic solvents and concentrated seawater (2012) (115)
- Advances in ultrahigh-throughput screening for directed enzyme evolution. (2019) (111)
- Phosphorothioate-based ligase-independent gene cloning (PLICing): An enzyme-free and sequence-independent cloning method. (2010) (111)
- Asymmetric reduction of ketones with recombinant E. coli whole cells in neat substrates. (2011) (106)
- Arginine deiminase, a potential anti-tumor drug. (2008) (104)
- Rational evolution of a medium chain-specific cytochrome P-450 BM-3 variant. (2001) (103)
- A nanocompartment system (Synthosome) designed for biotechnological applications. (2006) (100)
- Directed evolution 2.0: improving and deciphering enzyme properties. (2015) (99)
- OmniChange: The Sequence Independent Method for Simultaneous Site-Saturation of Five Codons (2011) (93)
- Directed evolution of oxygenases: screening systems, success stories and challenges. (2007) (84)
- P450 in biotechnology: zinc driven omega-hydroxylation of p-nitrophenoxydodecanoic acid using P450 BM-3 F87A as a catalyst. (2000) (83)
- Cloning and characterization of a thermostable and halo-tolerant endoglucanase from Thermoanaerobacter tengcongensis MB4 (2010) (80)
- Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries. (2007) (78)
- Advances in protease engineering for laundry detergents. (2015) (78)
- Directed evolution of a thermophilic endoglucanase (Cel5A) into highly active Cel5A variants with an expanded temperature profile. (2011) (76)
- Directed evolution of a highly active Yersinia mollaretii phytase (2012) (75)
- Protein Engineering – An Option for Enzymatic Biofuel Cell Design (2010) (74)
- Challenges and advances in the field of self-assembled membranes. (2013) (73)
- Laboratory evolution of P450 BM3 for mediated electron transfer yielding an activity-improved and reductase-independent variant. (2007) (70)
- A hybrid ring-opening metathesis polymerization catalyst based on an engineered variant of the β-barrel protein FhuA. (2013) (68)
- Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution (2013) (68)
- To get what we aim for – progress in diversity generation methods (2013) (67)
- Regioselective o-hydroxylation of monosubstituted benzenes by P450 BM3. (2013) (67)
- Directed evolution of glucose oxidase from Aspergillus niger for ferrocenemethanol-mediated electron transfer. (2007) (66)
- A screening system for the directed evolution of epoxygenases: importance of position 184 in P450 BM3 for stereoselective styrene epoxidation. (2006) (66)
- Stereoselective epoxidation of the last double bond of polyunsaturated fatty acids by human cytochromes P450 (2010) (65)
- A rhodium complex-linked β-barrel protein as a hybrid biocatalyst for phenylacetylene polymerization. (2012) (65)
- Directed laccase evolution for improved ionic liquid resistance (2013) (64)
- Biofunctional Microgel-Based Fertilizers for Controlled Foliar Delivery of Nutrients to Plants. (2017) (63)
- A Whole Cell E. coli Display Platform for Artificial Metalloenzymes: Poly(phenylacetylene) Production with a Rhodium–Nitrobindin Metalloprotein (2018) (61)
- PTEN-inhibition by zinc ions augments interleukin-2-mediated Akt phosphorylation. (2014) (58)
- Direct oxidation of cycloalkanes to cycloalkanones with oxygen in water. (2013) (58)
- Functionalized nanocompartments (synthosomes) with a reduction-triggered release system. (2008) (57)
- P450 monooxygenase in biotechnology. I. Single-step, large-scale purification method for cytochrome P450 BM-3 by anion-exchange chromatography. (1999) (56)
- Sensitive Assay for Laboratory Evolution of Hydroxylases toward Aromatic and Heterocyclic Compounds (2005) (55)
- A roadmap to directed enzyme evolution and screening systems for biotechnological applications. (2013) (55)
- A Highly Active Biohybrid Catalyst for Olefin Metathesis in Water: Impact of a Hydrophobic Cavity in a β-Barrel Protein (2015) (54)
- An efficient transformation method for Bacillus subtilis DB104 (2012) (54)
- Cost-Effective Whole-Cell Assay for Laboratory Evolution of Hydroxylases in Escherichia coli (2001) (53)
- Computer-Aided Protein Directed Evolution: a Review of Web Servers, Databases and other Computational Tools for Protein Engineering (2012) (52)
- Structural Insight into Enantioselective Inversion of an Alcohol Dehydrogenase Reveals a "Polar Gate" in Stereorecognition of Diaryl Ketones. (2018) (50)
- Targeting microplastic particles in the void of diluted suspensions. (2019) (49)
- Engineering of the E. coli Outer Membrane Protein FhuA to overcome the Hydrophobic Mismatch in Thick Polymeric Membranes (2011) (49)
- Understanding a mechanism of organic cosolvent inactivation in heme monooxygenase P450 BM-3. (2007) (48)
- Ionic liquid and deep eutectic solvent-activated CelA2 variants generated by directed evolution (2014) (47)
- Transversion‐enriched sequence saturation mutagenesis (SeSaM‐Tv+): A random mutagenesis method with consecutive nucleotide exchanges that complements the bias of error‐prone PCR (2008) (47)
- Reengineered glucose oxidase for amperometric glucose determination in diabetes analytics. (2013) (46)
- Cellulolytic RoboLector – towards an automated high-throughput screening platform for recombinant cellulase expression (2017) (46)
- A Flow Cytometry–Based Screening System for Directed Evolution of Proteases (2011) (46)
- A fluorescent hydrogel-based flow cytometry high-throughput screening platform for hydrolytic enzymes. (2014) (44)
- Ionic liquid effects on the activity of monooxygenase P450 BM-3 (2008) (44)
- Anchor peptides: A green and versatile method for polypropylene functionalization (2017) (43)
- Towards the Evolution of Artificial Metalloenzymes-A Protein Engineer's Perspective. (2019) (43)
- Directed Evolution of a Bacterial Laccase (CueO) for Enzymatic Biofuel Cells. (2019) (43)
- Modification of the fatty acid specificity of cytochrome P450 BM-3 from Bacillus megaterium by directed evolution: a validated assay (2001) (43)
- Laboratory Evolution of P450 BM‐3 for Mediated Electron Transfer (2006) (42)
- Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails (2020) (42)
- Multi-step biocatalytic depolymerization of lignin (2017) (41)
- Extending the substrate scope of a Baeyer–Villiger monooxygenase by multiple-site mutagenesis (2014) (41)
- Hybrid ruthenium ROMP catalysts based on an engineered variant of β-barrel protein FhuA ΔCVF(tev) : effect of spacer length. (2015) (40)
- Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations (2014) (40)
- In Vitro Double Oxidation of n-Heptane with Direct Cofactor Regeneration (2013) (39)
- Exploring the Protein Stability Landscape: Bacillus subtilis Lipase A as a Model for Detergent Tolerance (2015) (39)
- Towards Understanding Directed Evolution: More than Half of All Amino Acid Positions Contribute to Ionic Liquid Resistance of Bacillus subtilis Lipase A (2015) (39)
- In vitro flow cytometry-based screening platform for cellulase engineering (2016) (38)
- Directed Evolution of P 450 BM 3 into a p‐Xylene Hydroxylase (2012) (38)
- Sortase-Mediated Surface Functionalization of Stimuli-Responsive Microgels. (2017) (38)
- Multi-site saturation by OmniChange yields a pH- and thermally improved phytase. (2014) (38)
- Electron transfer pathways in a light, oxygen, voltage (LOV) protein devoid of the photoactive cysteine (2017) (37)
- Flow Cytometer-Based High-Throughput Screening System for Accelerated Directed Evolution of P450 Monooxygenases (2012) (37)
- Photophysics of the LOV-Based Fluorescent Protein Variant iLOV-Q489K Determined by Simulation and Experiment. (2016) (36)
- Polymersome surface decoration by an EGFP fusion protein employing Cecropin A as peptide "anchor". (2012) (36)
- SeSaM‐Tv‐II Generates a Protein Sequence Space that is Unobtainable by epPCR (2011) (36)
- KnowVolution of the Polymer-Binding Peptide LCI for Improved Polypropylene Binding (2018) (36)
- Computer‐Assisted Recombination (CompassR) Teaches us How to Recombine Beneficial Substitutions from Directed Evolution Campaigns (2019) (36)
- Tunable Enzymatic Activity and Enhanced Stability of Cellulase Immobilized in Biohybrid Nanogels. (2016) (35)
- QM/MM Calculations Revealing the Resting and Catalytic States in Zinc-Dependent Medium-Chain Dehydrogenases/Reductases (2015) (34)
- Cloning, expression, and characterization of a thermostable glucoamylase from Thermoanaerobacter tengcongensis MB4 (2010) (34)
- Benzylic hydroxylation of aromatic compounds by P450 BM3 (2013) (33)
- Disulfide Bond Engineering of an Endoglucanase from Penicillium verruculosum to Improve Its Thermostability (2019) (33)
- Design of an activity and stability improved carbonyl reductase from Candida parapsilosis. (2013) (33)
- Ultrahigh-throughput screening system for directed glucose oxidase evolution in yeast cells. (2011) (33)
- Functionalized nanocompartments (Synthosomes): Limitations and prospective applications in industrial biotechnology (2006) (33)
- Mediated electron transfer with P450cin (2010) (32)
- Toward understanding the inactivation mechanism of monooxygenase P450 BM-3 by organic cosolvents: a molecular dynamics simulation study. (2006) (32)
- Expression of the Zn2+-containing hydroxynitrile lyase from flax (Linum usitatissimum) in Pichia pastoris— utilization of the recombinant enzyme for enzymatic analysis and site-directed mutagenesis (1998) (31)
- Directed evolution of polypropylene and polystyrene binding peptides (2018) (31)
- Surface charge engineering of a Bacillus gibsonii subtilisin protease (2013) (31)
- KnowVolution of a Fungal Laccase toward Alkaline pH (2019) (31)
- Directed arginine deiminase evolution for efficient inhibition of arginine-auxotrophic melanomas (2015) (31)
- Are transversion mutations better? A Mutagenesis Assistant Program analysis on P450 BM‐3 heme domain (2007) (30)
- Challenges of the genetic code for exploring sequence space in directed protein evolution (2007) (30)
- Cavity Size Engineering of a β-Barrel Protein Generates Efficient Biohybrid Catalysts for Olefin Metathesis (2018) (30)
- A Potential Antitumor Drug (Arginine Deiminase) Reengineered for Efficient Operation under Physiological Conditions (2010) (29)
- Nanocompartments with a pH release system based on an engineered OmpF channel protein (2011) (29)
- A Competitive Flow Cytometry Screening System for Directed Evolution of Therapeutic Enzyme. (2015) (29)
- Iterative key-residues interrogation of a phytase with thermostability increasing substitutions identified in directed evolution (2015) (28)
- An Enzymatic Route to α-Tocopherol Synthons: Aromatic Hydroxylation of Pseudocumene and Mesitylene with P450 BM3. (2017) (28)
- Are Directed Evolution Approaches Efficient in Exploring Nature’s Potential to Stabilize a Lipase in Organic Cosolvents? (2017) (28)
- Temperature effects on structure and dynamics of the psychrophilic protease subtilisin S41 and its thermostable mutants in solution. (2011) (28)
- Machine learning-assisted enzyme engineering. (2020) (28)
- Chemoenzymatic route to β-blockers via 3-hydroxy esters (1996) (28)
- Membrane-mimetic dendrimersomes engulf living bacteria via endocytosis. (2019) (27)
- Reengineered carbonyl reductase for reducing methyl-substituted cyclohexanones. (2013) (27)
- Who's Who? Allocation of Carbonyl Reductase Isoenzymes from Candida parapsilosis by Combining Bio‐ and Computational Chemistry (2012) (27)
- Whole-cell double oxidation of n-heptane. (2014) (27)
- Directed Evolution of Hyaluronic Acid Synthase from Pasteurella multocida towards High‐Molecular‐Weight Hyaluronic Acid (2018) (27)
- A loop engineering strategy improves laccase lcc2 activity in ionic liquid and aqueous solution (2018) (27)
- Water-Soluble Reactive Copolymers Based on Cyclic N-Vinylamides with Succinimide Side Groups for Bioconjugation with Proteins (2015) (26)
- Expression of Arginine Deiminase from Pseudomonas plecoglossicida CGMCC2039 in Escherichia coli and Its Anti-Tumor Activity (2009) (26)
- Phosphorothioate-based DNA recombination: an enzyme-free method for the combinatorial assembly of multiple DNA fragments. (2012) (26)
- Prearranged glycosides, 2. Intramolecular glycosylation of prearranged saccharides as a novel strategy for the construction of β‐L‐rhamnosidic linkages (1995) (26)
- Rhodium‐Complex‐Linked Hybrid Biocatalyst: Stereo‐Controlled Phenylacetylene Polymerization within an Engineered Protein Cavity (2014) (26)
- Directed Evolution of Subtilisin E into a Highly Active and Guanidinium Chloride‐ and Sodium Dodecylsulfate‐Tolerant Protease (2012) (26)
- How To Engineer Ionic Liquids Resistant Enzymes: Insights from Combined Molecular Dynamics and Directed Evolution Study (2019) (26)
- First steps towards a Zn/Co(III)sep-driven P450 BM3 reactor (2011) (26)
- Vanadium bromoperoxidase-coupled fluorescent assay for flow cytometry sorting of glucose oxidase gene libraries in double emulsions (2012) (25)
- Sequence saturation mutagenesis with tunable mutation frequencies. (2005) (25)
- How to Engineer Organic Solvent Resistant Enzymes: Insights from Combined Molecular Dynamics and Directed Evolution Study (2020) (25)
- Casting epPCR (cepPCR): A simple random mutagenesis method to generate high quality mutant libraries (2017) (25)
- Rapid evolution of arginine deiminase for improved anti-tumor activity (2011) (25)
- Structural and dynamic properties of cytochrome P450 BM‐3 in pure water and in a dimethylsulfoxide/water mixture (2005) (24)
- Artificial Diels–Alderase based on the transmembrane protein FhuA (2016) (24)
- A nanophosphor‐based method for selective DNA recovery in Synthosomes (2006) (24)
- Enzyme Hydration Determines Resistance in Organic Cosolvents (2020) (24)
- The role of active-site Phe87 in modulating the organic co-solvent tolerance of cytochrome P450 BM3 monooxygenase. (2012) (23)
- Grafting PNIPAAm from β-barrel shaped transmembrane nanopores. (2016) (23)
- Enzyme–Polyelectrolyte Complexes Boost the Catalytic Performance of Enzymes (2018) (23)
- Progress toward a bicarbonate-based microalgae production system. (2021) (22)
- A bifunctional dermaseptin–thanatin dipeptide functionalizes the crop surface for sustainable pest management (2019) (22)
- An electrochemical microtiter plate for parallel spectroelectrochemical measurements (2013) (22)
- MIXed plastics biodegradation and UPcycling using microbial communities: EU Horizon 2020 project MIX-UP started January 2020 (2021) (22)
- P-LinK: A method for generating multicomponent cytochrome P450 fusions with variable linker length. (2014) (22)
- Engineering Enhanced Pore Sizes Using FhuA Δ1-160 from E. coli Outer Membrane as Template. (2017) (22)
- A first continuous 4-aminoantipyrine (4-AAP)-based screening system for directed esterase evolution (2015) (21)
- Less Unfavorable Salt Bridges on the Enzyme Surface Result in More Organic Cosolvent Resistance (2021) (21)
- Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications (2011) (21)
- Sortase-Mediated High-Throughput Screening Platform for Directed Enzyme Evolution. (2018) (21)
- Matter‐tag: A universal immobilization platform for enzymes on polymers, metals, and silicon‐based materials (2020) (21)
- Mediated electron transfer with monooxygenases—Insight in interactions between reduced mediators and the co-substrate oxygen (2014) (20)
- Biocatalytic hydroxylation of n-butane with in situ cofactor regeneration at low temperature and under normal pressure (2012) (20)
- Recent Advances in Directed Phytase Evolution and Rational Phytase Engineering (2017) (20)
- Depolymerization of Laccase-Oxidized Lignin in Aqueous Alkaline Solution at 37 °C (2019) (20)
- A thermostable flavin-based fluorescent protein from Chloroflexus aggregans: a framework for ultra-high resolution structural studies. (2019) (20)
- Molecular understanding of sterically controlled compound release through an engineered channel protein (FhuA) (2010) (20)
- Protein Engineering of the Antitumor Enzyme PpADI for Improved Thermal Resistance (2014) (19)
- Alcohol dehydrogenase stabilization by additives under industrially relevant reaction conditions (2014) (19)
- MAP(2.0)3D: a sequence/structure based server for protein engineering. (2012) (19)
- Combinatorial optimization of synthetic operons for the microbial production of p-coumaryl alcohol with Escherichia coli (2015) (19)
- 2-Methyltetrahydrofuran and cyclopentylmethylether: two green solvents for efficient purification of membrane proteins like FhuA. (2013) (19)
- Loop engineering reveals the importance of active-site-decorating loops and gating residue in substrate affinity modulation of arginine deiminase (an anti-tumor enzyme). (2018) (19)
- Ultrahigh‐throughput screening system for directed polymer binding peptide evolution (2019) (19)
- Biocatalyst Immobilization by Anchor Peptides on an Additively Manufacturable Material (2019) (19)
- First Insights on Organic Cosolvent Effects on FhuA Wildtype and FhuA Δ1-159 (2012) (19)
- Reactive Copolymers Based on N-Vinyl Lactams with Pyridyl Disulfide Side Groups via RAFT Polymerization and Postmodification via Thiol–Disulfide Exchange Reaction (2016) (19)
- Rapid and Oriented Immobilization of Laccases on Electrodes via a Methionine-Rich Peptide (2021) (19)
- Direktoxidation von Cycloalkanen zu Cycloalkanonen mit Sauerstoff in Wasser (2013) (19)
- Stimuli-Responsive Poly( N-Vinyllactams) with Glycidyl Side Groups: Synthesis, Characterization, and Conjugation with Enzymes. (2019) (18)
- Rapid and Robust Coating Method to Render Polydimethylsiloxane Surfaces Cell Adhesive. (2019) (18)
- Coupling of electrochemical and optical measurements in a microtiter plate for the fast development of electro enzymatic processes with P450s (2013) (18)
- Engineered phytases for emerging biotechnological applications beyond animal feeding (2019) (18)
- Engineering and emerging applications of artificial metalloenzymes with whole cells (2021) (18)
- Critical effect of proline on thermostability of endoglucanase II from Penicillium verruculosum (2019) (18)
- Phytase-Based Phosphorus Recovery Process for 20 Distinct Press Cakes (2020) (17)
- CaLB Catalyzed Conversion of ε-Caprolactone in Aqueous Medium. Part 1: Immobilization of CaLB to Microgels (2016) (17)
- A computational protocol to predict suitable redox mediators for substitution of NAD(P)H in P450 monooxygenases (2013) (17)
- Reversible Deactivation of Enzymes by Redox-Responsive Nanogel Carriers. (2016) (17)
- Fluorescent Assay for Directed Evolution of Perhydrolases (2012) (17)
- Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance (2018) (17)
- Ionic liquid activated Bacillus subtilis lipase A variants through cooperative surface substitutions (2015) (17)
- Insight into the redox partner interaction mechanism in cytochrome P450BM-3 using molecular dynamics simulations. (2014) (16)
- Activity prediction of substrates in NADH-dependent carbonyl reductase by docking requires catalytic constraints and charge parameterization of catalytic zinc environment (2015) (16)
- Olefin metathesis catalysts embedded in β-barrel proteins: creating artificial metalloproteins for olefin metathesis (2018) (16)
- Light-induced structural changes in a short light, oxygen, voltage (LOV) protein revealed by molecular dynamics simulations—implications for the understanding of LOV photoactivation (2015) (15)
- A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels. (2015) (15)
- A hydroquinone-specific screening system for directed P450 evolution (2018) (15)
- Directed evolution of P450cin for mediated electron transfer (2017) (15)
- Selective Functionalization of Microgels with Enzymes by Sortagging. (2019) (15)
- P450 BM3 crystal structures reveal the role of the charged surface residue Lys/Arg184 in inversion of enantioselective styrene epoxidation. (2013) (15)
- KnowVolution of a GH5 Cellulase from Penicillium verruculosum to Improve Thermal Stability for Biomass Degradation (2020) (15)
- Conformational dynamics of active site loop in Escherichia coli phytase. (2010) (15)
- KnowVolution Campaign of an Aryl Sulfotransferase Increases Activity toward Cellobiose. (2018) (15)
- Residue K556‐A Light Triggerable Gatekeeper to Sterically Control Translocation in FhuA (2011) (14)
- CompassR yields highly organic solvent-tolerant enzymes through recombination of compatible substitutions. (2020) (14)
- Gerichtete Evolution ermöglicht das Design von maßgeschneiderten Proteinen zur nachhaltigen Produktion von Chemikalien und Pharmazeutika (2018) (14)
- Effects of ionic liquids on the reaction kinetics of a laccase–mediator system (2014) (14)
- A whole cell biocatalyst for double oxidation of cyclooctane (2016) (14)
- Regioselektive o‐Hydroxylierung monosubstituierter Benzole mit P450 BM3 (2013) (14)
- Protein consensus-based surface engineering (ProCoS): a computer-assisted method for directed protein evolution. (2016) (14)
- How to engineer glucose oxidase for mediated electron transfer (2018) (13)
- One‐Pot Two‐Step Chemoenzymatic Cascade for the Synthesis of a Bis‐benzofuran Derivative (2019) (13)
- Enzyme-Compatible Dynamic Nanoreactors from Electrostatically Bridged Like-Charged Surfactants and Polyelectrolytes. (2018) (13)
- MicroGelzymes: pH-Independent Immobilization of Cytochrome P450 BM3 in Microgels. (2020) (13)
- A robust protocol for directed aryl sulfotransferase evolution toward the carbohydrate building block GlcNAc (2018) (13)
- Increasing protein production by directed vector backbone evolution (2013) (13)
- The Sequence Saturation Mutagenesis (SeSaM) method. (2014) (13)
- Preparative‐Scale Production of Testosterone Metabolites by Human Liver Cytochrome P450 Enzyme 3A4 (2020) (13)
- Chemogenetic Evolution of a Peroxidase-like Artificial Metalloenzyme (2021) (12)
- Improved microscale cultivation of Pichia pastoris for clonal screening (2018) (12)
- A robust bacterial assay for high-throughput screening of human 4-hydroxyphenylpyruvate dioxygenase inhibitors (2019) (12)
- dRTP and dPTP a complementary nucleotide couple for the Sequence Saturation Mutagenesis (SeSaM) method (2012) (12)
- An engineered outer membrane pore enables an efficient oxygenation of aromatics and terpenes (2016) (12)
- Directed sortase A evolution for efficient site-specific bioconjugations in organic co-solvents. (2018) (12)
- FhuA deletion variant Δ1-159 overexpression in inclusion bodies and refolding with Polyethylene-Poly(ethylene glycol) diblock copolymer. (2011) (12)
- Chemoenzymatic cascade for stilbene production from cinnamic acid catalyzed by ferulic acid decarboxylase and an artificial metathease (2019) (12)
- Anchor Peptide-Mediated Surface Immobilization of a Grubbs-Hoveyda-Type Catalyst for Ring-Opening Metathesis Polymerization. (2019) (12)
- Biadhesive Peptides for Assembling Stainless Steel and Compound Loaded Micro-Containers. (2019) (11)
- 2-Methyl-2,4-pentanediol (MPD) boosts as detergent-substitute the performance of ß-barrel hybrid catalyst for phenylacetylene polymerization (2017) (11)
- Exploring the mineralization of hydrophobins at a liquid interface (2012) (11)
- Incorporation of a Cp*Rh(III)-dithiophosphate Cofactor with Latent Activity into a Protein Scaffold Generates a Biohybrid Catalyst Promoting C(sp2)-H Bond Functionalization. (2020) (11)
- Directed OmniChange Evolution Converts P450 BM3 into an Alkyltrimethylammonium Hydroxylase. (2018) (11)
- Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases (2016) (11)
- Investigation of Structural Determinants for the Substrate Specificity in the Zinc‐Dependent Alcohol Dehydrogenase CPCR2 from Candida parapsilosis (2015) (11)
- Unraveling the effects of amino acid substitutions enhancing lipase resistance to an ionic liquid: a molecular dynamics study. (2018) (11)
- Directed evolution of VanR biosensor specificity in yeast (2020) (11)
- Systematically Scrutinizing the Impact of Substitution Sites on Thermostability and Detergent Tolerance for Bacillus subtilis Lipase A (2020) (11)
- Conformational Dynamics of the FMN-Binding Reductase Domain of Monooxygenase P450BM-3. (2013) (11)
- Rationalizing perhydrolase activity of aryl-esterase and subtilisin Carlsberg mutants by molecular dynamics simulations of the second tetrahedral intermediate state (2010) (11)
- Rational surface engineering of an arginine deiminase (an antitumor enzyme) for increased PEGylation efficiency (2019) (10)
- What's My Substrate? Computational Function Assignment of Candida parapsilosis ADH5 by Genome Database Search, Virtual Screening, and QM/MM Calculations (2016) (10)
- PyPEF - An Integrated Framework for Data-Driven Protein Engineering (2021) (10)
- A Comparative Reengineering Study of cpADH5 through Iterative and Simultaneous Multisite Saturation Mutagenesis (2018) (10)
- Ein Durchmusterungssystem für die gelenkte Evolution von Epoxygenasen: Bedeutung der Position 184 in P 450 BM3 für die stereoselektive Epoxidierung von Styrol (2006) (10)
- A Semi-Rationally Engineered Bacterial Pyrrolysyl-tRNA Synthetase Genetically Encodes Phenyl Azide Chemistry. (2018) (10)
- Turning a Killing Mechanism into an Adhesion and Antifouling Advantage (2019) (10)
- Effects of Proline Substitutions on the Thermostable LOV Domain from Chloroflexus aggregans (2020) (10)
- Nano-thin walled micro-compartments from transmembrane protein-polymer conjugates. (2017) (9)
- Insights on activity and stability of subtilisin E towards guanidinium chloride and sodium dodecylsulfate. (2014) (9)
- A High-Throughput Screening Method to Reengineer DNA Polymerases for Random Mutagenesis (2014) (9)
- Kill&Repel Coatings: The Marriage of Antifouling and Bactericidal Properties to Mitigate and Treat Wound Infections (2021) (9)
- Chiral separation of d/l-arginine with whole cells through an engineered FhuA nanochannel. (2019) (9)
- In situ monitoring of membrane protein insertion into block copolymer vesicle membranes and their spreading via potential-assisted approach. (2019) (9)
- Sugar-Improved Enzymatic Synthesis of Biodiesel with Yarrowia lipolytica Lipase 2 (2017) (9)
- Redirecting catalysis from proteolysis to perhydrolysis in subtilisin Carlsberg. (2013) (9)
- Screening through the PLICable promoter toolbox enhances protein production in Escherichia coli (2016) (9)
- MuteinDB: the mutein database linking substrates, products and enzymatic reactions directly with genetic variants of enzymes (2012) (8)
- OmniChange: simultaneous site saturation of up to five codons. (2014) (8)
- Directed evolution of an acid Yersinia mollaretii phytase for broadened activity at neutral pH (2018) (8)
- Directed Evolution of P450 BM3 towards Functionalization of Aromatic O-Heterocycles (2019) (8)
- Tunnel engineering for modulating the substrate preference in cytochrome P450BsβHI (2021) (8)
- Reengineering of subtilisin Carlsberg for oxidative resistance (2013) (8)
- Sortase-Mediated Ligation of Purely Artificial Building Blocks (2018) (8)
- The molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins (2021) (8)
- Biohybrid catalysts for sequential one-pot reactions based on an engineered transmembrane protein (2019) (8)
- A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC. (2020) (8)
- Engineering of Laccase CueO for Improved Electron Transfer in Bioelectrocatalysis by Semi‐Rational Design (2020) (7)
- Amino acid substitutions in random mutagenesis libraries: lessons from analyzing 3000 mutations (2017) (7)
- Theoretical Model of the Protochlorophyllide Oxidoreductase from a Hierarchy of Protocols. (2018) (7)
- A p-nitrothiophenolate screening system for the directed evolution of a two-component epoxygenase (StyAB) (2008) (7)
- Aqueous ionic liquids redistribute local enzyme stability via long-range perturbation pathways (2021) (7)
- Biocatalytic microgels (μ-Gelzymes): synthesis, concepts, and emerging applications (2020) (7)
- Engineering of Highly Selective Variants of Parvibaculum lavamentivorans Alcohol Dehydrogenase (2014) (6)
- Construction of a whole-cell biohybrid catalyst using a Cp*Rh(III)-dithiophosphate complex as a precursor of a metal cofactor. (2021) (6)
- Biofunktionale Mikrogel-basierte Dünger zur kontrollierten Blattdüngung mit Nährstoffen auf Pflanzen (2017) (6)
- Critical assessment of structure-based approaches to improve protein resistance in aqueous ionic liquids by enzyme-wide saturation mutagenesis (2021) (6)
- Cyclotrimerization of phenylacetylene catalyzed by a cobalt half-sandwich complex embedded in an engineered variant of transmembrane protein FhuA. (2018) (6)
- The Mutagenesis Assistant Program. (2014) (6)
- Directed Evolution of a Cp*RhIII‐Linked Biohybrid Catalyst Based on a Screening Platform with Affinity Purification (2020) (6)
- Unraveling Binding Effects of Cobalt(II) Sepulchrate with the Monooxygenase P450 BM-3 Heme Domain Using Molecular Dynamics Simulations. (2016) (6)
- Designed Streptococcus pyogenes Sortase A Accepts Branched Amines as Nucleophiles in Sortagging. (2020) (6)
- Consensus model of a cyanobacterial light-dependent protochlorophyllide oxidoreductase in its pigment-free apo-form and photoactive ternary complex (2019) (6)
- Challenges and Advances in the Field of Self-Assembled Membranes (2013) (5)
- Endogenous Nitric Oxide-Releasing Microgel Coating Prevents Clot Formation on Oxygenator Fibers Exposed to In Vitro Blood Flow (2022) (5)
- Recombinant RNA Polymerase from Geobacillus sp. GHH01 as tool for rapid generation of metagenomic RNAs using in vitro technologies (2017) (5)
- CompassR-guided recombination unlocks design principles to stabilize lipases in ILs with minimal experimental efforts (2021) (5)
- A Photoclick‐Based High‐Throughput Screening for the Directed Evolution of Decarboxylase OleT (2020) (5)
- Understanding substrate binding and the role of gatekeeping residues in PigC access tunnels. (2021) (5)
- Can constraint network analysis guide the identification phase of KnowVolution? A case study on improved thermostability of an endo-β-glucanase (2020) (5)
- Inversion of cpADH5 Enantiopreference and Altered Chain Length Specificity for Methyl 3-Hydroxyalkanoates. (2017) (5)
- Anchor peptides promote degradation of mixed plastics for recycling. (2021) (5)
- Modulating the Coupling Efficiency of P450 BM3 by Controlling Water Diffusion through Access Tunnel Engineering (2021) (5)
- Comparison of Candida antarctica Lipase B Variants for Conversion of ε-Caprolactone in Aqueous Medium—Part 2 (2018) (5)
- Chemoenzymatic Route to β‐Blockers via 3‐Hydroxy Esters. (1996) (5)
- Engineered P450 BM3 and cpADH5 coupled cascade reaction for β-oxo fatty acid methyl ester production in whole cells. (2020) (5)
- A 96-multiplex capillary electrophoresis screening platform for product based evolution of P450 BM3 (2019) (5)
- A filter paper-based assay for laboratory evolution of hydrolases and dehydrogenases. (2006) (5)
- Correction: A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels. (2015) (5)
- Consensus model of a cyanobacterial light-dependent protochlorophyllide oxidoreductase in its pigment-free apo-form and photoactive ternary complex. (2019) (4)
- Three multi-enzyme cascade pathways for conversion of C1 to C2/C4 compounds (2022) (4)
- Enzyme mimetic microgel coating for endogenous nitric oxide mediated inhibition of platelet activation. (2021) (4)
- FhuA–Grubbs–Hoveyda Biohybrid Catalyst Embedded in a Polymer Film Enables Catalysis in Neat Substrates (2020) (4)
- Directed Evolution 2.0: Improving and Deciphering Enzyme Properties (2015) (4)
- Directed Evolution of a Bacterial Laccase (CueO) for Enzymatic Biofuel Cells (2019) (4)
- Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization (2020) (4)
- How Does Surface Charge Engineering of Bacillus subtilis Lipase A Improve Ionic Liquid Resistance? Lessons Learned from Molecular Dynamics Simulations (2022) (4)
- Application cases of biological transformation in manufacturing technology (2020) (4)
- Directed aryl sulfotransferase evolution toward improved sulfation stoichiometry on the example of catechols (2019) (4)
- Phytase blends for enhanced phosphorous mobilization of deoiled seeds (2021) (4)
- Flexibility Regulation of Loops Surrounding the Tunnel Entrance in Cytochrome P450 Enhanced Substrate Access Substantially (2022) (3)
- Reporter Immobilization Assay (REIA) for Bioconjugating Reactions. (2016) (3)
- High-Throughput Screening Assays for Lipolytic Enzymes. (2018) (3)
- Advances in directed monooxygenase evolution : from diversity generation and flow cytometry screening to tailor-made monooxygenases (2013) (3)
- An artificial ruthenium-containing β-barrel protein for alkene-alkyne coupling reaction. (2021) (3)
- Natural Product Diversification by One‐Step Biocatalysis using Human P450 3A4 (2021) (3)
- Evolution of E. coli Phytase Toward Improved Hydrolysis of Inositol Tetraphosphate (2022) (3)
- Insights on intermolecular FMN-heme domain interaction and the role of linker length in cytochrome P450cin fusion proteins (2020) (3)
- Generation of phytase chimeras with low sequence identities and improved thermal stability. (2021) (3)
- A peptide‐based coating toolbox to enable click chemistry on polymers, metals, and silicon through sortagging (2021) (3)
- Combinatorial InVitroFlow‐assisted mutagenesis (CombIMut) yields a 41‐fold improved CelA2 cellulase (2022) (3)
- Polar Substitutions on the Surface of a Lipase Substantially Improve Tolerance in Organic Solvents (2022) (3)
- Engineering Proteases for Industrial Applications (2016) (3)
- Structure and Cooperativity in Substrate-Enzyme Interactions: Perspectives on Enzyme Engineering and Inhibitor Design. (2022) (3)
- Novel technique for high throughput measurement of active monooxygenase concentration. (2017) (2)
- Auf dem Weg zur Evolution artifizieller Metalloenzyme – aus einem Protein‐Engineering‐Blickwinkel (2019) (2)
- High-Yield Synthesis of Enantiopure 1,2-Amino Alcohols from l-Phenylalanine via Linear and Divergent Enzymatic Cascades (2022) (2)
- Protein Nanopore Membranes Prepared by a Simple Langmuir-Schaefer Approach. (2021) (2)
- Fatty Acid Hydroxylations Using P450 Monooxygenases (2005) (2)
- Ternary Complex Formation and Photoactivation of a Photoenzyme Results in Altered Protein Dynamics. (2019) (2)
- A plea for the integration of Green Toxicology in sustainable bioeconomy strategies –biosurfactants and microgel-based pesticide release systems as examples (2021) (2)
- Conditioning of Feed Material Prior to Feeding: Approaches for a Sustainable Phosphorus Utilization (2022) (2)
- Cover Feature: Directed Evolution of Hyaluronic Acid Synthase from Pasteurella multocida towards High-Molecular-Weight Hyaluronic Acid (ChemBioChem 13/2018) (2018) (2)
- A robust bacterial high-throughput screening system to evaluate single nucleotide polymorphisms of human homogentisate 1,2-dioxygenase in the context of alkaptonuria (2022) (2)
- KnowVolution of prodigiosin ligase PigC towards condensation of short-chain prodiginines (2021) (2)
- Display of functional nucleic acid polymerase on Escherichia coli surface and its application in directed polymerase evolution (2020) (2)
- In Silico and Experimental ADAM17 Kinetic Modeling as Basis for Future Screening System for Modulators (2022) (2)
- Unraveling the Mechanism and Kinetics of Binding of an LCI-eGFP-Polymer for Antifouling Coatings. (2021) (2)
- Protein Engineering by Efficient Sequence Space Exploration Through Combination of Directed Evolution and Computational Design Methodologies (2021) (1)
- Loop engineering of aryl sulfotransferase B for improving catalytic performance in regioselective sulfation (2020) (1)
- High Throughput Screening Method for Engineering P450 Towards Terminal Hydroxylation of Fatty Acids (2019) (1)
- The molecular basis and enzyme engineering strategies for improvement of coupling efficiency in cytochrome P450s. (2022) (1)
- FACS Based High Throughput Screening Systems for Gene Libraries in Double Emulsions (2012) (1)
- Optimized Hemolysin Type 1 Secretion System in Escherichia coli by Directed Evolution of the Hly Enhancer Fragment and Including a Terminator Region (2022) (1)
- BioAdhere: tailor-made bioadhesives for epiretinal visual prostheses. (2022) (1)
- Anchor peptides as innovative adjuvants reduce rain wash-off, but do not impair photosynthetic activity or cause oxidative damage in apple leaves (2020) (1)
- High-throughput quantification of ochronotic pigment formation in Escherichia coli to evaluate the potency of human 4-hydroxyphenylpyruvate dioxygenase inhibitors in multi-well format (2020) (1)
- Using a bio-economic farm model to evaluate the economic potential and pesticide load reduction of the greenRelease technology (2022) (1)
- Engineering of FhuA into an ideal scaffold for metallozymes (2013) (1)
- Enzyme-Compatible Dynamic Nanoreactors from Electrostatically Bridged Like-Charged Surfactants and Polyelectrolytes (2018) (1)
- Engineering of cytochrome P450 monooxygenases for application in phenol synthesis (2014) (1)
- Reprint of: Application cases of biological transformation in manufacturing technology (2021) (1)
- CHAPTER 3:Channel Protein FhuA as a Promising Biomolecular Scaffold for Bioconjugates (2015) (1)
- Directed evolution of arginine deiminase (ADI) for anti-tumor application (2010) (1)
- Expression and Refolding of the Plant Chitinase From Drosera capensis for Applications as a Sustainable and Integrated Pest Management (2021) (1)
- Chemogenetic engineering of nitrobindin toward an artificial epoxygenase (2021) (1)
- Protein engineering of the monooxygenase P450 BM3 toward improved performance in allylic hydroxylation (2020) (1)
- Whole-cell screening of oxidative enzymes using genetically encoded sensors (2021) (1)
- Biotechnological production of food-grade polyphosphate from deoiled seeds and bran (2023) (1)
- Modulare Enzym-Kaskaden zur Synthese von Glykanepitopen (2015) (1)
- DECHEMA/VAAM-Fachgruppe „Biotransformationen“ (2013) (1)
- Untersuchung verschiedener Ein- und Dreikomponenten-P450-Systeme für die Anwendung in der Biokatalyse (2015) (1)
- Künstliche Metalloproteine für die Olefinmetathese (2018) (1)
- Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization. (2020) (1)
- Crystallographic insights into a cobalt (III) sepulchrate based alternative cofactor system of P450 BM3 monooxygenase. (2018) (1)
- Using Molecular Simulation to Guide Protein Engineering for Biocatalysis in Organic Solvents. (2021) (1)
- Fe(III)-complex mediated bacterial cell surface immobilization of eGFP and enzymes. (2021) (1)
- Rational Design Yields Molecular Insights on Leaf-Binding of Anchor Peptides. (2022) (1)
- Directed evolution of Arabidopsis thaliana carotenoid cleavage dioxygenase 1 (2011) (1)
- Structural and mechanistic insight into spectral tuning in flavin-binding fluorescent proteins (2021) (1)
- Novel polyphosphate analytics for the development of biotechnological polyphosphate production (2020) (1)
- MIXed Plastics Biodegradation and UPcycling Using Microbial Communities: The EU Horizon 2020 Project MIX-UP (2021) (0)
- Self-Sufficient In Vitro Multi-Enzyme Cascade for Efficient Synthesis of Danshensu from l-DOPA. (2022) (0)
- Neat Substrate System: Expansion of the concept to a broad range of single enzymes and enzyme cascades (2012) (0)
- Crystal structure of P450 BM3 5F5 heme domain variant complexed with styrene (dataset II) (2013) (0)
- Development of a flow cytometer-based in vitro compartmentalization screening platform for directed protein evolution (2014) (0)
- Directed subtilisin E evolution into a chaotolerant protease (2013) (0)
- Electron donor system for enzyme and its application in the biochemical implementation of substrates (2000) (0)
- Engineering a lipase for organic cosolvent resistance - How do current directed evolution approaches compete with the potential that nature offers? (2017) (0)
- Bifunctional Fusion Proteins for Plant Protection - BiFuProts - (2016) (0)
- Microwave-Assisted Biocatalysis Employing Glycosidases (2010) (0)
- Crystal structure of P450 BM3 heme domain M7 variant (2017) (0)
- Substrate thiophosphorylation by Arabidopsis mitogen-activated protein (2016) (0)
- Cover Picture: Rhodium‐Complex‐Linked Hybrid Biocatalyst: Stereo‐Controlled Phenylacetylene Polymerization within an Engineered Protein Cavity (ChemCatChem 5/2014) (2014) (0)
- Rational design yields molecular insights on leaf binding of the anchor peptide Macaque Histatin (2022) (0)
- KnowVolution: Redesigning enzymes for innovations (2017) (0)
- Directed sortase evolution for site-specific protein engineering and surface functionalization (2019) (0)
- Increasing protein production by directed vector backbone evolution (2013) (0)
- Lessons on directed evolution of hydrolases and glucose oxidase (2014) (0)
- Erratum zu „Bestimmung der Stabilität und Enantioselektivität von Lipasen“ (2018) (0)
- A hybrid model combining evolutionary probability and machine learning leverages data-driven protein engineering (2022) (0)
- Generierung von ≤ 3,2 Mio. Proteinvarianten in fokussiertem Mutageneseexperiment (2014) (0)
- Protein engineering of a therapeutic enzyme arginine deiminase (2015) (0)
- Deciphering rationales that determine the resistance of a lipase towards non-conventional media (2015) (0)
- Verfahren zur enzymatischen umsetzung von alkanen (2009) (0)
- Leistungsverbesserte Proteasevariante [Offenlegungsschrift] (2012) (0)
- Identification of stable and enantioselective lipases for biotechnological applications (2018) (0)
- Entwicklung von Oxidationskatalysatoren für die luftsauerstoffbasierte Bleiche und Untersuchung der zugrunde liegenden molekularen Mechanismen (2020) (0)
- Microscale cultivation of Pichia pastoris accelerates bioprocess development (2016) (0)
- Cover Feature: Computer‐Assisted Recombination (CompassR) Teaches us How to Recombine Beneficial Substitutions from Directed Evolution Campaigns (Chem. Eur. J. 3/2020) (2020) (0)
- Engineering of subtilisin proteases for detergent applications (2013) (0)
- Investigations on transglycosylation reactions of glycosidases and glycosynthases for the syntheses of (poly-)N-acetyllactosamine glycans (2020) (0)
- P450 BM3 F87A in complex with DMSO (2011) (0)
- Reaction and protein engineering employing a carbonyl reductase from candida parapsilosis (2013) (0)
- Optimizing Microbial Screenings Using Controlled-Release Systems (2010) (0)
- Efficient phosphate recovery from agro waste streams by enzyme, strain, and process engineering (2015) (0)
- Protein engineering for polyethylene terephthalate degradation (2016) (0)
- Chiral separation of D / L-arginine with whole cells through an engineered FhuA 3 nanochannel 4 5 (2019) (0)
- Durchflusszytometrie zum Auffinden verbesserter und neuer Enzyme (2013) (0)
- Phytoalexine und bifunktionale Fusionsproteine für den Pflanzenschutz (2016) (0)
- Recombination of Compatible Substitutions by 2GenReP and InSiReP. (2021) (0)
- From natural to artificial metalloproteins – Challenges and opportunities (2019) (0)
- Crystal structure of mutant nitrobindin M75L/H76L/Q96C/V128W/M148L/H158L (NB9) from Arabidopsis thaliana (2014) (0)
- Design of biohybrid catalysts for metathesis reactions (2015) (0)
- Evolutionary Engineering of a Cp*Rh(III) Complex-Linked Artificial Metalloenzyme with a Chimeric β-Barrel Protein Scaffold (2023) (0)
- Boosting the performance of a protease with polymers and surfactants (2019) (0)
- Crystal structure of P450 BM3 5F5K heme domain variant complexed with styrene (2013) (0)
- Directed protein evolution as a tool to innovate gene therapy for hereditary tyrosinemia type 1 (2020) (0)
- Structural insights of mediated electron transfer by P450 BM3 monooxygenase (2014) (0)
- Bestimmung der Stabilität und Enantioselektivität von Lipasen (2018) (0)
- Millionenfach beschleunigte Evolution für maβgeschneiderte Proteine (2018) (0)
- Mono-oxygenases cytochrome p450 modified. (2000) (0)
- Biofunktionalisierung von Biomaterialoberflächen mit poly-LacNAc und den Galektinen-1, -3 und -8 (2015) (0)
- Gelenkte Evolution von Epoxygenasen (2006) (0)
- Directed aryl sulfotransferase evolution toward improved sulfation stoichiometry on the example of catechols (2019) (0)
- Synthesis of neo-glycoproteins for binding studies and scavenging of Clostridium difficile toxin A - of microgels and biosensors (2021) (0)
- Insertion of Cecropin A and reconstitution of bacterial outer membrane protein FhuA variants in polymeric membranes (2011) (0)
- Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases (2016) (0)
- Supporting a novel plant protection technology by molecular dynamics simulations (2020) (0)
- NOVEL FLOW CYTOMETER-BASED PLATFORMS (2016) (0)
- Directed evolution of a subtilisin Carlsberg variant towards increased oxidative stability (2012) (0)
- Front Cover: Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization (Chem. Eur. J. 60/2020) (2020) (0)
- A Flow Cytometry-Based Ultrahigh-Throughput Screening Method for Directed Evolution of Oxidases. (2023) (0)
- Millionenfach beschleunigte Evolution für maβgeschneiderte Proteine (2018) (0)
- Bifunctional peptides (BiFuPeps) as fungicides for agriculture (2016) (0)
- An efficient transformation method for Bacillus subtilis DB104 (2012) (0)
- Engineering of prodigiosin ligase pigC towards production of short-chain prodiginines (2021) (0)
- SeSaM (Sequence Saturation Mutagenesis) : eine Methode zur Sättigungsmutagenese eines Genes (2006) (0)
- Optimization of Glucose oxidase towards oxygen independency and high mediator activity for amperometric glucose determination in diabetes analytics (2015) (0)
- Protein engineering of laccases towards higher pH (2019) (0)
- Bifunctional Fusion Proteins for Plant Protection (2016) (0)
- A fluorescent polymer shell-based enzyme screening platform for hydrolases using flow cytometry (2014) (0)
- Phytoalexins and bifunctional fusion proteins for plant protection (2016) (0)
- Directed evolution and computational analysis of ionic liquid resistant lipase variants (2016) (0)
- Ionic liquid and deep eutectic solvent-activated CelA2 variants generated by directed evolution (2014) (0)
- Plastibodies for multiplexed detection and sorting of microplastic particles in high-throughput. (2022) (0)
- Kill&Repel Coatings: The Marriage of Antifouling and Bactericidal Properties to Mitigate and Treat Wound Infections (Adv. Funct. Mater. 9/2022) (2022) (0)
- Durchflusszytometrie zum Auffinden verbesserter und neuer Enzyme (2013) (0)
- Generierung von ≤ 3,2 Mio. Proteinvarianten in fokussiertem Mutageneseexperiment (2014) (0)
- Cover Feature: One-Pot Two-Step Chemoenzymatic Cascade for the Synthesis of a Bis-benzofuran Derivative (Eur. J. Org. Chem. 37/2019) (2019) (0)
- Monitoring, characterizing, and influencing the poly(γ-glutamic acid) production by Bacillus licheniformis (2016) (0)
- Selecting of a cytochrome P450cam SeSaM library with 3-chloroindole and endosulfan - Identification of mutants that dehalogenate 3-chloroindole. (2018) (0)
- Directed Evolution of DNA Polymerases for Advancement of the SeSaM Mutagenesis Method and Biotransformations with P450 BM3 Monooxygenase (2015) (0)
- Directed arginine deiminase evolution for efficient inhibition of arginine-auxotrophic melanomas (2014) (0)
- Etablierung eines Systems aus Cysteinmutanten der Phosphoglycerat-Kinase für Entfaltungsstudien mit Einzelmolekül-FRET (2017) (0)
- Herstellung von neuartigen Monomeren auf Basis von Kohlenhydraten als Baustein für wasserlösliche Polymere (2020) (0)
- Identifizierung und Entwicklung von effizienten anionischen Tensiden basierend auf Produkten der Lignocellulose-Wertschöpfung (2019) (0)
- Mutant nitrobindin M75L/H76L/Q96C/M148L (NB4H) from Arabidopsis thaliana with cofactor MnPPIX (2021) (0)
- Engineering of Laccase CueO for Improved Electron Transfer in Bioelectrocatalysis by Semi-Rational Design. (2020) (0)
- Lipases for use in detergents and cleaning agents (2016) (0)
- DECHEMA/VAAM-Fachgruppe „Biotransformationen“ (2013) (0)
- Electron transfer pathways in a light, oxygen, voltage (LOV) protein devoid of the photoactive cysteine (2017) (0)
- Erratum zu „Bestimmung der Stabilität und Enantioselektivität von Lipasen“ (2018) (0)
- Bifunktionelle Fusionspeptide und Mikrogel-basierte Abgabesysteme für die Pflanzengesundheit (2018) (0)
- Inside Cover: A Potential Antitumor Drug (Arginine Deiminase) Reengineered for Efficient Operation under Physiological Conditions (ChemBioChem 16/2010) (2010) (0)
- Crystal structure of P450 BM3 heme domain variant complexed with Co(III)Sep (2016) (0)
- E. Molecular Design of Metalloproteins (2009) (0)
- Functional polymersomes (Synthosomes) for bioconversions and selective product recovery (2008) (0)
- Frontispiece: Biofunctional Microgel‐Based Fertilizers for Controlled Foliar Delivery of Nutrients to Plants (2017) (0)
- Crystal structure of mutant nitrobindin M75L/H76L/Q96C/M148L/H158L covalently linked with [Rh(Cp-Mal)(COD)] (NB4-Rh) from Arabidopsis thaliana (2014) (0)
- Methods and materials for manipulation of nucleic acids (2012) (0)
- Development of a Modular Biosensor System for Rapid Pathogen Detection (2018) (0)
- Surface charge engineering of a Bacillus gibsonii subtilisin protease (2012) (0)
- Protein engineering of FhuA Δ1-160 variants for controlled release of compounds (2018) (0)
- Functionalizing plant surface for sustained plant protection (2018) (0)
- Directed evolution of a highly active Yersinia mollaretii phytase (2011) (0)
- Procédé de conversion enzymatique d'alcanes (2009) (0)
- Flow cytometry based high throughput screening system for screening and improving industrially important enzymes (2009) (0)
- P450 BM3 Heme domain in complex with DMSO (2007) (0)
- Tailor-made sortase reactions (2019) (0)
- Engineered adhesion peptides for functionalization of natural surfaces, polymers, and metal alloys (2019) (0)
- Functional microgel coatings (2021) (0)
- Structure protects function - An enabler for the functionalization of component surfaces by biohybrid coatings (2022) (0)
- Electrochemical Study of B11 Glucose Oxidase from Direct Protein Evolution (2008) (0)
- Iterative key-residues interrogation of a phytase with thermostability increasing substitutions identified in directed evolution (2015) (0)
- Recombination of Single Beneficial Substitutions Obtained from Protein Engineering by Computer-Assisted Recombination (CompassR). (2022) (0)
- Engineered phytases for emerging biotechnological applications beyond animal feeding (2019) (0)
- Amino acid substitutions in random mutagenesis libraries: lessons from analyzing 3000 mutations (2017) (0)
- Extending the substrate scope of a Baeyer–Villiger monooxygenase by multiple-site mutagenesis (2013) (0)
- Tunnel Engineering for Modulating the Substrate Preference in Decarbonylase P450BsβHI (2020) (0)
- INTRAMOLECULAR GLYCOSYLATION OF PREARRANGED SACCHARIDES AS A NOVEL STRATEGY FOR THE CONSTRUCTION OF BETA -L-RHAMNOSIDIC LINKAGES (1996) (0)
- Schaltbare Proteine für interaktive Materialien (2011) (0)
- Biochemie und Molekularbiologie 2008 (2009) (0)
- Multi-step biocatalytic depolymerization of lignin (2017) (0)
- A novel metagenome-derived viral RNA polymerase and its application in a cell-free expression system for metagenome screening (2022) (0)
- Entwicklung neuartiger Bioindikatoren für die Dampfsterilisation (2017) (0)
- Enzyme Hydration: How to Retain Resistance in Ionic Liquids (2022) (0)
- Method for adapting a hydrolytic enzyme to a the hydrolytic enzyme stabilizing component (2011) (0)
- A hydroquinone-specific screening system for directed P450 evolution (2018) (0)
- A first continuous 4-aminoantipyrine (4-AAP)-based screening system for directed esterase evolution (2015) (0)
- Improved microscale cultivation of Pichia pastoris for clonal screening (2018) (0)
- New cytochrome P450 monooxygenases and their use for the oxidation of organic compounds (2000) (0)
- Directed evolution of a subtilisin Carlsberg variant towards improved perhydrolytic activity for industrial application (2012) (0)
- Bestimmung der Stabilität und Enantioselektivität von Lipasen (2018) (0)
- Directed evolution of an acid Yersinia mollaretii phytase for broadened activity at neutral pH (2018) (0)
- Optimization of cellulase CelA2 with improved performance in high ionic strength for the production of biofuels (2013) (0)
- Preparative Production of Functionalized (N- and O-Heterocyclic) Polycyclic Aromatic Hydrocarbons by Human Cytochrome P450 3A4 in a Bioreactor (2022) (0)
- Reactive stimuli-responsive poly(vinyl lactam) copolymers for design of nanogels based protein and drug carriers (2016) (0)
- A Screening Method for P450 BM3 Mutant Libraries Using Multiplexed Capillary Electrophoresis for Detection of Enzymatically Converted Compounds. (2022) (0)
- Wie Enzym-Engineering das Waschen optimiert (2016) (0)
- Insights into the structural basis for activity and selectivity of P450 BM3 monooxygenase (2013) (0)
- Multi-enzymatic cascade reactions for oxyfunctionalization of alkanes employing P450 BM3 monooxygenases (2014) (0)
- Activation of a Photoenzyme Results in Modified Structure and Dynamics (2020) (0)
- Cellulolytic RoboLector – towards an automated high-throughput screening platform for recombinant cellulase expression (2017) (0)
- Biohybrid catalysts for alkene metathesis (2017) (0)
- Directed evolution of polymerases and its application in sequence saturation mutagenesis (2021) (0)
- Coupled reaction of glycosyltransferase and sucrose synthase for high-yielding and cost-effective synthesis of rosin (2023) (0)
- Front Cover: Engineering of Laccase CueO for Improved Electron Transfer in Bioelectrocatalysis by Semi‐Rational Design (Chem. Eur. J. 22/2020) (2020) (0)
- Cover Feature: Directed Evolution of a Cp*Rh III ‐Linked Biohybrid Catalyst Based on a Screening Platform with Affinity Purification (ChemBioChem 4/2021) (2021) (0)
- Leaf surface-immobilized bifunctional fusion proteins provide improved crop protection (2017) (0)
- Potential applications of laccase-mediator-systems for biorefinery purposes (2016) (0)
- Optimizing the phosphorus cycle in the sugar beet production process by phytase supplement (2017) (0)
- Development of high-throughput optical measuring systems for P450 monooxygenases (2018) (0)
- Benzylic Hydroxylation of Aromatic Compounds by P450 BM3. (2014) (0)
- Crystal structure of P450 BM3 5F5 heme domain variant (2013) (0)
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