Virginijus Šikšnys
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Lithuanian biochemist
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Biology
Virginijus Šikšnys's Degrees
- PhD Biochemistry Vilnius University
Why Is Virginijus Šikšnys Influential?
(Suggest an Edit or Addition)According to Wikipedia, Virginijus Šikšnys is a Lithuanian biochemist and a professor at Vilnius University. He is a chief scientist at the Vilnius University Institute of Biotechnology. Biography Šikšnys studied organic chemistry at Vilnius University, receiving his Masters in 1978, then moved to Lomonosov Moscow State University, where he studied enzyme kinetics and received Candidate of Sciences degree in 1983. From 1982 till 1993 he worked at the Institute of Applied Enzymology in Vilnius. In 1993 he was a visiting scientist in Robert Huber’s laboratory at the Max-Planck-Institut für Biochemie, Martinsried, Germany. Since 1995 Šikšnys is the chief scientist and head of the Department of Protein-DNA Interactions at the Vilnius University Institute of Biotechnology, since 2006 – professor at Vilnius University and a member of the Lithuanian Academy of Sciences, since 2007 – chair of the Institute of Biotechnology Council.
Virginijus Šikšnys's Published Works
Number of citations in a given year to any of this author's works
Total number of citations to an author for the works they published in a given year. This highlights publication of the most important work(s) by the author
Published Works
- Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria (2012) (2147)
- Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants (2019) (899)
- The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli (2011) (750)
- Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes (2014) (373)
- Cas3 is a single‐stranded DNA nuclease and ATP‐dependent helicase in the CRISPR/Cas immune system (2011) (371)
- A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems (2017) (297)
- Programmable RNA shredding by the type III-A CRISPR-Cas system of Streptococcus thermophilus. (2014) (228)
- In vitro reconstitution of Cascade‐mediated CRISPR immunity in Streptococcus thermophilus (2013) (228)
- crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus (2013) (192)
- Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome. (2016) (186)
- Spatiotemporal Control of Type III-A CRISPR-Cas Immunity: Coupling DNA Degradation with the Target RNA Recognition. (2016) (181)
- Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements (2015) (163)
- Structure of the tetrameric restriction endonuclease NgoMIV in complex with cleaved DNA (2000) (161)
- Directional R-Loop Formation by the CRISPR-Cas Surveillance Complex Cascade Provides Efficient Off-Target Site Rejection. (2015) (136)
- Type III CRISPR-Cas Immunity: Major Differences Brushed Aside. (2017) (103)
- Molecular mechanisms of CRISPR-mediated microbial immunity (2013) (103)
- Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15 A resolution. (1996) (100)
- Novel Subtype of Type IIs Restriction Enzymes (2000) (98)
- PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage (2020) (96)
- Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific nuclease. (2005) (95)
- The Cfr10I restriction enzyme is functional as a tetramer. (1999) (91)
- Single-molecule dynamics of the DNA-EcoRII protein complexes revealed with high-speed atomic force microscopy. (2009) (80)
- DNA cleavage reactions by type II restriction enzymes that require two copies of their recognition sites. (2001) (78)
- The metal-independent type IIs restriction enzyme BfiI is a dimer that binds two DNA sites but has only one catalytic centre. (2003) (70)
- Crystal structure of MunI restriction endonuclease in complex with cognate DNA at 1.7 Å resolution (1999) (69)
- Lactase non-persistence is directed by DNA variation-dependent epigenetic aging (2016) (67)
- A catalogue of biochemically diverse CRISPR-Cas9 orthologs (2020) (67)
- Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequence. (2002) (62)
- CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement (2019) (62)
- How the BfiI restriction enzyme uses one active site to cut two DNA strands (2003) (60)
- Five‐stranded β‐sheet sandwiched with two α‐helices: A structural link between restriction endonucleases EcoRI and EcoRV (1994) (60)
- Nucleotide flips determine the specificity of the Ecl18kI restriction endonuclease (2006) (60)
- RNA-dependent DNA endonuclease Cas9 of the CRISPR system: Holy Grail of genome editing? (2013) (59)
- BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site (2018) (58)
- Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease (2021) (51)
- Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants (2019) (50)
- The recognition domain of the methyl-specific endonuclease McrBC flips out 5-methylcytosine (2012) (49)
- Structure-based redesign of the catalytic/metal binding site of Cfr10I restriction endonuclease reveals importance of spatial rather than sequence conservation of active centre residues. (1998) (47)
- DNA binding specificity of MunI restriction endonuclease is controlled by pH and calcium ions: involvement of active site carboxylate residues. (1997) (46)
- Alternative arrangements of catalytic residues at the active sites of restriction enzymes (2002) (45)
- Conversion of the tetrameric restriction endonuclease Bse634I into a dimer: oligomeric structure-stability-function correlations. (2005) (44)
- Restriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically (2007) (44)
- Evolutionary and functional classification of the CARF domain superfamily, key sensors in prokaryotic antivirus defense. (2020) (43)
- Generation of the BfiI restriction endonuclease from the fusion of a DNA recognition domain to a non-specific nuclease from the phospholipase D superfamily. (2004) (43)
- Direct visualization of the EcoRII-DNA triple synaptic complex by atomic force microscopy. (2007) (43)
- Use of specific oligonucleotide duplexes to stimulate cleavage of refractory DNA sites by restriction endonucleases. (1993) (40)
- Central base pair flipping and discrimination by PspGI (2008) (38)
- Nucleotide flipping by restriction enzymes analyzed by 2-aminopurine steady-state fluorescence (2007) (37)
- Structural mechanisms for the 5′-CCWGG sequence recognition by the N- and C-terminal domains of EcoRII (2009) (36)
- Monomeric restriction endonuclease BcnI in the apo form and in an asymmetric complex with target DNA. (2007) (35)
- Biochemical and mutational analysis of EcoRII functional domains reveals evolutionary links between restriction enzymes (2006) (34)
- Simultaneous binding of three recognition sites is necessary for a concerted plasmid DNA cleavage by EcoRII restriction endonuclease. (2006) (34)
- CAATTG-specific restriction-modification munI genes from Mycoplasma: sequence similarities between R.MunI and R.EcoRI. (1994) (33)
- Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor (2021) (31)
- Site-directed mutagenesis of putative active site residues of MunI restriction endonuclease: replacement of catalytically essential carboxylate residues triggers DNA binding specificity. (1997) (30)
- Plasmid DNA cleavage by MunI restriction enzyme: single-turnover and steady-state kinetic analysis. (1999) (30)
- Evidence for substrate-assisted catalysis in the DNA cleavage of several restriction endonucleases. (1995) (30)
- Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System (2019) (28)
- Tetrameric restriction enzymes: expansion to the GIY-YIG nuclease family (2007) (28)
- DnaQ exonuclease‐like domain of Cas2 promotes spacer integration in a type I‐E CRISPR‐Cas system (2018) (28)
- Multiple phage resistance systems inhibit infection via SIR2-dependent NAD+ depletion (2021) (28)
- Targeted gene editing by transfection of in vitro reconstituted Streptococcus thermophilus Cas9 nuclease complex (2015) (27)
- DNA synapsis through transient tetramerization triggers cleavage by Ecl18kI restriction enzyme (2010) (26)
- Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells (2021) (26)
- Different enzymes with similar structures involved in Mg2+-mediated polynucleotidyl transfer (1995) (26)
- A novel mechanism for the scission of double-stranded DNA: BfiI cuts both 3′–5′ and 5′–3′ strands by rotating a single active site (2010) (26)
- Site-specific DNA transesterification catalyzed by a restriction enzyme (2007) (24)
- Programmable DNA cleavage in vitro by Cas9. (2013) (24)
- The crystal structure of the rare-cutting restriction enzyme SdaI reveals unexpected domain architecture. (2006) (24)
- Harnessing the natural diversity and in vitro evolution of Cas9 to expand the genome editing toolbox. (2017) (24)
- Structural basis of DNA target recognition by the B3 domain of Arabidopsis epigenome reader VAL1 (2018) (23)
- Structural insight into the specificity of the B3 DNA-binding domains provided by the co-crystal structure of the C-terminal fragment of BfiI restriction enzyme (2014) (22)
- Genetic Dissection of the Type III-A CRISPR-Cas System Csm Complex Reveals Roles of Individual Subunits. (2019) (22)
- Methods for decoding Cas9 protospacer adjacent motif (PAM) sequences: A brief overview. (2017) (22)
- Structure and Function of the Tetrameric Restriction Enzymes (2004) (21)
- Target site cleavage by the monomeric restriction enzyme BcnI requires translocation to a random DNA sequence and a switch in enzyme orientation (2011) (20)
- Specific DNA recognition by the type II restriction endonuclease MunI: the effect of pH. (2001) (20)
- Time-resolved fluorescence studies of nucleotide flipping by restriction enzymes (2009) (20)
- PAM recognition by miniature CRISPR-Cas14 triggers programmable double-stranded DNA cleavage (2019) (19)
- Five-stranded beta-sheet sandwiched with two alpha-helices: a structural link between restriction endonucleases EcoRI and EcoRV. (1994) (19)
- Short prokaryotic Argonautes provide defence against incoming mobile genetic elements through NAD+ depletion (2021) (18)
- Allosteric communication network in the tetrameric restriction endonuclease Bse634I. (2006) (17)
- Type III-A CRISPR-associated protein Csm6 degrades cyclic hexa-adenylate activator using both CARF and HEPN domains (2020) (17)
- 5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage (2020) (16)
- Activity and structure of EcoKMcrA (2018) (16)
- DNA recognition by Arabidopsis transcription factors ABI3 and NGA1 (2018) (16)
- Oligomeric structure diversity within the GIY-YIG nuclease family. (2009) (16)
- Sequence similarity among type-II restriction endonucleases, related by their recognized 6-bp target and tetranucleotide-overhang cleavage. (1995) (16)
- Rewiring Cas9 to Target New PAM Sequences. (2016) (15)
- Molecular scissors under light control (2010) (15)
- How PspGI, catalytic domain of EcoRII and Ecl18kI acquire specificities for different DNA targets (2008) (14)
- Photocaged variants of the MunI and PvuII restriction enzymes. (2011) (14)
- Ca2+-ions stimulate DNA binding specificity of Cfr10I restriction enzyme. (1998) (13)
- Degenerate sequence recognition by the monomeric restriction enzyme: single mutation converts BcnI into a strand-specific nicking endonuclease (2011) (12)
- The recognition domain of the BpuJI restriction endonuclease in complex with cognate DNA at 1.3-A resolution. (2008) (12)
- Targeted RNA Knockdown by a Type III CRISPR-Cas Complex in Zebrafish (2020) (11)
- The dynamics of the monomeric restriction endonuclease BcnI during its interaction with DNA (2017) (11)
- HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation. (2020) (11)
- A pipeline for characterization of novel Cas9 orthologs. (2019) (11)
- Catalytic and binding properties of restriction endonuclease Cfr9I. (1993) (11)
- Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA (2016) (10)
- Structure-guided sequence specificity engineering of the modification-dependent restriction endonuclease LpnPI (2015) (10)
- Catalytic activity control of restriction endonuclease--triplex forming oligonucleotide conjugates. (2012) (10)
- 5’ modifications to CRISPR Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage (2020) (10)
- Decision-Making in Cascade Complexes Harboring crRNAs of Altered Length (2019) (9)
- CRISPR adaptation from a structural perspective. (2020) (9)
- A novel mechanism for the scission of double-stranded DNA : BfiI cuts both 3 0 – 5 0 and 5 0 – 3 0 strands by rotating a single active site (2009) (9)
- DNA cleavage by CgII and NgoAVII requires interaction between N- and R-proteins and extensive nucleotide hydrolysis (2014) (9)
- Crystal structure of the R-protein of the multisubunit ATP-dependent restriction endonuclease NgoAVII (2014) (8)
- Restriction endonucleases that resemble a component of the bacterial DNA repair machinery (2007) (8)
- Restriction endonuclease BpuJI specific for the 5′-CCCGT sequence is related to the archaeal Holliday junction resolvase family (2007) (8)
- Cas3 nuclease-helicase activity assays. (2015) (7)
- Functional significance of protein assemblies predicted by the crystal structure of the restriction endonuclease BsaWI (2015) (7)
- Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease (2012) (7)
- Restriction enzyme Ecl18kI-induced DNA looping dynamics by single-molecule FRET. (2014) (6)
- UbaLAI is a monomeric Type IIE restriction enzyme (2017) (6)
- Role of the reactive cysteine residue in restriction endonuclease Cfr9I. (1992) (4)
- Targeted RNA knockdown by crRNA guided Csm in zebrafish (2017) (4)
- The link between restriction endonuclease fidelity and oligomeric state: A study with Bse634I (2012) (3)
- A quantitative model for the dynamics of target recognition and off-target rejection by the CRISPR-Cas Cascade complex (2022) (3)
- The H-subunit of the restriction endonuclease CglI contains a prototype DEAD-Z1 helicase-like motor (2018) (3)
- Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family (2018) (3)
- Template-directed addition of nucleosides to DNA by the BfiI restriction enzyme (2008) (3)
- Applications of the Versatile CRISPR-Cas Systems (2013) (3)
- A new family of CRISPR‐type V nucleases with C‐rich PAM recognition (2022) (2)
- Genetic and epigenetic factors in lactose intolerance: lessons for aging diseases (2017) (2)
- Biochemically diverse CRISPR-Cas9 orthologs (2020) (2)
- CgII cleaves DNA using a mechanism distinct from other ATP-dependent restriction endonucleases (2017) (2)
- An Engineered SS Bridge Blocks the Conformational Change Required for the Nuclease Activity of BfiI. (2015) (2)
- DNA cleavage by CgII and NgoAVII requires interaction between N- and R-proteins and extensive nucleotide hydrolysis (2015) (1)
- CRYSTAL STRUCTURE OF MUNI RESTRICTION ENDONUCLEASE IN COMPLEX WITH COGNATE DNA (2000) (1)
- Mis-annotation of TnpB: case of TaRGET-ABE (2023) (1)
- TnpB structure reveals minimal functional core of Cas12 nuclease family (2023) (1)
- Dynamics of R-loop formation by CRISPR-Cas12f1 effector complexes (2022) (0)
- Crystal structure of restriction endonuclease AgeI (2016) (0)
- Crystal structure of pre-specific restriction endonuclease BsaWI-DNA complex (2015) (0)
- Cas1-Cas2-Csn2-DNA dimer complex from the Type II-A CRISPR-Cas system (2019) (0)
- Crystal structure of specific restriction endonuclease AgeI-DNA complex (2016) (0)
- Restriction Endonuclease BCNI-Cognate DNA Substrate Complex (2007) (0)
- The generation of animal models to design novel gene correction approaches for Pompe disease (2008) (0)
- A comparison of palindrome and pseudopalindrome cutters (2006) (0)
- Crystal structure of mono-AMPylated HEPN(R46E) toxin in complex with MNT antitoxin (2020) (0)
- Cas1-Cas2-Csn2-DNA complex from the Type II-A CRISPR-Cas system (2019) (0)
- Crystal structure of UbaLAI restriction endonuclease B3 domain domain (mutant L24M L53M L95M) with cognate DNA (2017) (0)
- Revealing Restriction Enzyme Dynamics With Fast-scan Afm (2009) (0)
- Molecular mechanisms of CRISPR-mediated microbial immunity (2013) (0)
- Energy landscapes of CRISPR/Cas R-loop formation from ultrafast twist measurements (2022) (0)
- Protein diffusion on DNA (2015) (0)
- Crystal structure of HEPN(R102A) toxin (2020) (0)
- Structure of SdaI restriction endonuclease (2006) (0)
- Dissecting the Target Recognition by CRISPR/Cas Effector Complexes with Ultrafast Twist Measurements (2021) (0)
- Dynamic DNA Target Proofreading in a CRISPR-Cas System (2015) (0)
- SURVEY AND SUMMARY Evolutionary and functional classification of the CARF domain superfamily, key sensors in prokaryotic antivirus defense (2020) (0)
- DNA recognition by restriction endonuclease AgeI (2011) (0)
- How do restriction enzymes acquire specificities for different target sites? [Abstract] (2008) (0)
- Dynamic interplay between target search and recognition for the Cascade surveillance complex of type I-E CRISPR-Cas systems (2022) (0)
- Correlated Single Molecule Twist and Fluorescence Measurements on CRISPR/Cas Systems (2018) (0)
- DNA binding domain of restriction endonuclease bound to DNA (2012) (0)
- Segment of the Cas1-Cas2-Csn2-DNA filament complex from the Type II-A CRISPR-Cas system (2019) (0)
- Investigating and Modelling the Target Recognition Dynamics of the CRISPR-Cas Surveillance Complex Cascade (2018) (0)
- Metal-depleted Ecl18kI in complex with uncleaved, modified DNA (2006) (0)
- Restriction Endonuclease BCNI in the Absence of DNA (2007) (0)
- Crystal structure of R-protein of NgoAVII restriction endonuclease (2014) (0)
- The energy landscape for R-loop formation by the CRISPR-Cas Cascade complex (2023) (0)
- Restriction endonuclease MvaI-cognate DNA substrate complex (2007) (0)
- Crystal structure of restriction endonuclease EcoRII N-terminal effector-binding domain in complex with cognate DNA (2009) (0)
- Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements (2015) (0)
- DNA flipping by restriction endonucleases (2009) (0)
- Genetic Characteristics of Lithuanian and Latvian Patients with Inflammatory Bowel Disease (2011) (0)
- Crystal structure of E.coli Cas1-Cas2 complex (2014) (0)
- Restriction endonuclease PspGI-substrate DNA complex (2008) (0)
- CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement (2019) (0)
- Crystal structure of restriction endonuclease EcoRII catalytic C-terminal domain in complex with cognate DNA (2009) (0)
- NotI is not boring. (2008) (0)
- DNA recognition domain of the cytosine modification-dependent restriction endonuclease LpnPI (2015) (0)
- Alternative binding mode of restriction endonuclease BcnI to cognate DNA (2010) (0)
- Crystal structure of Type IIP restriction endonuclease PfoI (2018) (0)
- Characterization of: Wed-186streptococcus thermophilus: Wed-186csm complex mediated Crispr interference in vitro: Wed-186 (2014) (0)
- RESTRICTION ENDONUCLEASE BcnI (WILD TYPE)-COGNATE DNA SUBSTRATE COMPLEX (2007) (0)
- Harnessing biodiversity of Cas9 to expand genome editing toolbox (2018) (0)
- Modification dependent EcoKMcrA restriction endonuclease (2018) (0)
- Response regulator PorX coordinates oligonucleotide signalling and gene expression to control the secretion of virulence factors (2022) (0)
- Unexpected domain architecture of Type IIP restriction endonuclease SdaI (2007) (0)
- Crystal structure of Type IIP restriction endonuclease PfoI with cognate DNA (2018) (0)
- TETRAMERIC RESTRICTION ENDONUCLEASE NGOMIV IN COMPLEX WITH CLEAVED DNA (2001) (0)
- Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease (2021) (0)
- Bse634I restriction endonuclease (2002) (0)
- Crystal structure of Type IIF restriction endonuclease NgoMIV with cognate uncleaved DNA (2011) (0)
- Crystal STRUCTURE OF RESTRICTION ENDONUCLEASE BFII C-TERMINAL RECOGNITION DOMAIN IN COMPLEX WITH COGNATE DNA (2014) (0)
- Crystal structure of HEPN(H107A-Y109F) toxin in complex with MNT antitoxin (2020) (0)
- From Restriction Enzymes to CRISPR: An Interview with Virginijus Siksnys. (2018) (0)
- Crystal structure of R.NgoAVII restriction endonuclease B3 domain with cognate DNA (2014) (0)
- Metal-depleted Ecl18kI in complex with uncleaved DNA (2006) (0)
- Structure of the BfiI restriction endonuclease (2005) (0)
- Crystal structure of Type IIP restriction endonuclease Kpn2I (2018) (0)
- Rebuilt and re-refined PDB entry 5yep: tri-AMPylated Shewanella oneidensis HEPN toxin in complex with MNT antitoxin (2020) (0)
- Crystal structure of restriction endonuclease BpuJI recognition domain in complex with cognate DNA (2008) (0)
- Double targeting restriction endonucleases: towards correction of the acid-α-glucosidase gene in Pompe disease (2019) (0)
- Crystal structure of di-AMPylated HEPN(R102A) toxin (2020) (0)
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