Huimin Zhao
#28,181
Most Influential Person Now
American chemist
Huimin Zhao's AcademicInfluence.com Rankings
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Chemistry
Huimin Zhao's Degrees
- PhD Chemistry California Institute of Technology
- Bachelors Chemistry Peking University
Why Is Huimin Zhao Influential?
(Suggest an Edit or Addition)According to Wikipedia, Huimin Zhao is the Steven L. Miller Chair Professor of Chemical and Biomolecular Engineering at the University of Illinois, Urbana-Champaign, as well as the leader of the Biosystems Design research theme in the Carl R. Woese Institute for Genomic Biology. His research focuses on directed evolution, metabolic engineering, bioinformatics and high throughput technologies.
Huimin Zhao's Published Works
Number of citations in a given year to any of this author's works
Total number of citations to an author for the works they published in a given year. This highlights publication of the most important work(s) by the author
Published Works
- Chromatin architecture reorganization during stem cell differentiation (2015) (1280)
- Molecular evolution by staggered extension process (StEP) in vitro recombination (1998) (721)
- DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways (2008) (629)
- High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System (2014) (425)
- Recent developments in pyridine nucleotide regeneration. (2003) (332)
- Regeneration of cofactors for use in biocatalysis. (2003) (318)
- Homology-integrated CRISPR-Cas (HI-CRISPR) system for one-step multigene disruption in Saccharomyces cerevisiae. (2015) (300)
- A Rewritable, Random-Access DNA-Based Storage System (2015) (284)
- Yeast Surface Display of Trifunctional Minicellulosomes for Simultaneous Saccharification and Fermentation of Cellulose to Ethanol (2009) (235)
- Customized optimization of metabolic pathways by combinatorial transcriptional engineering (2012) (232)
- Selective Elimination of Mitochondrial Mutations in the Germline by Genome Editing (2015) (225)
- A highly sensitive selection method for directed evolution of homing endonucleases (2005) (221)
- Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering. (2009) (219)
- Transcription activator‐like effector nucleases (TALENs): A highly efficient and versatile tool for genome editing (2013) (218)
- Engineering microbial factories for synthesis of value-added products (2011) (215)
- Improving and repurposing biocatalysts via directed evolution. (2015) (215)
- CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters (2017) (204)
- Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system (2017) (202)
- Directed evolution of enzymes and biosynthetic pathways. (2006) (198)
- Recent advances in metabolic engineering of Saccharomyces cerevisiae: New tools and their applications. (2018) (185)
- Activation and Characterization of a Cryptic Polycyclic Tetramate Macrolactam Biosynthetic Gene Cluster (2013) (182)
- Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains. (2014) (177)
- CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973 (2016) (167)
- A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae. (2016) (162)
- Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae (2012) (159)
- Directed evolution of enzymes and pathways for industrial biocatalysis. (2002) (157)
- Recent advances in the bioremediation of persistent organic pollutants via biomolecular engineering (2005) (156)
- Recent advances in DNA assembly technologies. (2014) (155)
- DNA-Based Storage: Trends and Methods (2015) (146)
- Building a global alliance of biofoundries (2019) (144)
- Relaxing the nicotinamide cofactor specificity of phosphite dehydrogenase by rational design. (2003) (144)
- Optimized TAL effector nucleases (TALENs) for use in treatment of sickle cell disease. (2012) (143)
- Automated multiplex genome-scale engineering in yeast (2017) (139)
- Efficient regeneration of NADPH using an engineered phosphite dehydrogenase (2007) (139)
- Biosynthesis of phloroglucinol. (2005) (135)
- Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold. (2013) (135)
- Biocatalyst development by directed evolution. (2012) (134)
- Directed Evolution of a Thermostable Phosphite Dehydrogenase for NAD(P)H Regeneration (2005) (134)
- High Throughput Screening and Selection Methods for Directed Enzyme Evolution (2014) (132)
- Engineered biosynthesis of natural products in heterologous hosts. (2015) (131)
- Seamless correction of the sickle cell disease mutation of the HBB gene in human induced pluripotent stem cells using TALENs. (2014) (129)
- Biocatalysis for the synthesis of pharmaceuticals and pharmaceutical intermediates. (2017) (128)
- Directed Evolution: Past, Present and Future. (2013) (121)
- Protein engineering in designing tailored enzymes and microorganisms for biofuels production. (2009) (119)
- In vitro reconstitution and crystal structure of p-aminobenzoate N-oxygenase (AurF) involved in aureothin biosynthesis (2008) (119)
- Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision (2018) (118)
- Systematic Identification of a Panel of Strong Constitutive Promoters from Streptomyces albus. (2015) (117)
- Engineering biological systems using automated biofoundries. (2017) (117)
- Development of a Synthetic Malonyl-CoA Sensor in Saccharomyces cerevisiae for Intracellular Metabolite Monitoring and Genetic Screening. (2015) (117)
- Recent advances in natural product discovery. (2014) (115)
- Recent advances in biocatalysis by directed enzyme evolution. (2006) (110)
- Discovery and characterization of novel d-xylose-specific transporters from Neurospora crassa and Pichia stipitis. (2010) (105)
- Directed evolution as a powerful synthetic biology tool. (2013) (101)
- Engineering microbial hosts for production of bacterial natural products. (2016) (100)
- Directed evolution of the nonribosomal peptide synthetase AdmK generates new andrimid derivatives in vivo. (2011) (100)
- New insight into the mechanism of methyl transfer during the biosynthesis of fosfomycin. (2007) (98)
- Directed evolution of specific receptor-ligand pairs for use in the creation of gene switches. (2005) (98)
- Heterologous production of fosfomycin and identification of the minimal biosynthetic gene cluster. (2006) (97)
- Metabolic engineering of Saccharomyces cerevisiae to improve 1-hexadecanol production. (2015) (96)
- Metabolic engineering of a Saccharomyces cerevisiae strain capable of simultaneously utilizing glucose and galactose to produce enantiopure (2R,3R)-butanediol. (2014) (91)
- Directed evolution: an evolving and enabling synthetic biology tool. (2012) (90)
- Heterologous Expression, Purification, and Characterization of a Highly Active Xylose Reductase from Neurospora crassa (2005) (87)
- Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler. (2011) (84)
- Breaking the silence: new strategies for discovering novel natural products. (2017) (83)
- Comparative Biochemical Characterization of Three Exolytic Oligoalginate Lyases from Vibrio splendidus Reveals Complementary Substrate Scope, Temperature, and pH Adaptations (2014) (82)
- New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters. (2016) (81)
- Combinatorial Design of a Highly Efficient Xylose-Utilizing Pathway in Saccharomyces cerevisiae for the Production of Cellulosic Biofuels (2012) (80)
- Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping (2019) (79)
- Reversal of the β-oxidation cycle in Saccharomyces cerevisiae for production of fuels and chemicals. (2015) (77)
- Utilizing an endogenous pathway for 1-butanol production in Saccharomyces cerevisiae. (2014) (76)
- Industrial biotechnology: Tools and applications (2009) (76)
- Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae. (2010) (76)
- Direct Conversion of Xylan to Ethanol by Recombinant Saccharomyces cerevisiae Strains Displaying an Engineered Minihemicellulosome (2012) (75)
- In vitro 'sexual' evolution through the PCR-based staggered extension process (StEP) (2006) (74)
- Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution. (2010) (74)
- Microbial synthesis of triacetic acid lactone (2006) (74)
- Promoter-proximal CTCF-binding promotes long-range-enhancer dependent gene activation (2021) (73)
- RNAi-assisted genome evolution in Saccharomyces cerevisiae for complex phenotype engineering. (2015) (70)
- Metabolic Engineering of Oleaginous Yeasts for Production of Fuels and Chemicals (2017) (70)
- Cloning, expression, and biochemical characterization of Streptomyces rubellomurinus genes required for biosynthesis of antimalarial compound FR900098. (2008) (69)
- Reconstitution and Characterization of Aminopyrrolnitrin Oxygenase, a Rieske N-Oxygenase That Catalyzes Unusual Arylamine Oxidation* (2005) (68)
- Programmable DNA-Guided Artificial Restriction Enzymes. (2017) (67)
- Recent developments in the application of P450 based biocatalysts. (2018) (67)
- Exploiting Issatchenkia orientalis SD108 for succinic acid production (2014) (66)
- Recent advances in combinatorial biosynthesis for drug discovery (2015) (66)
- Direct observation of TALE protein dynamics reveals a two-state search mechanism (2015) (65)
- Using natural products for drug discovery: the impact of the genomics era (2017) (65)
- Towards a fully automated algorithm driven platform for biosystems design (2019) (64)
- Protein design for pathway engineering. (2014) (64)
- Activation of silent biosynthetic gene clusters using transcription factor decoys (2018) (63)
- Selective reduction of xylose to xylitol from a mixture of hemicellulosic sugars. (2010) (63)
- Alginate Lyases from Alginate-Degrading Vibrio splendidus 12B01 Are Endolytic (2015) (62)
- Insights into Cell-Free Conversion of CO2 to Chemicals by a Multienzyme Cascade Reaction (2018) (60)
- Genome-Scale Consequences of Cofactor Balancing in Engineered Pentose Utilization Pathways in Saccharomyces cerevisiae (2011) (59)
- Characterization of the Substrate Specificity of PhlD, a Type III Polyketide Synthase from Pseudomonas fluorescens* (2006) (57)
- Design and engineering of intracellular‐metabolite‐sensing/regulation gene circuits in Saccharomyces cerevisiae (2016) (57)
- DNA assembly techniques for next-generation combinatorial biosynthesis of natural products (2014) (56)
- Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae (2016) (55)
- Investigating xylose metabolism in recombinant Saccharomyces cerevisiae via 13C metabolic flux analysis (2013) (55)
- Evolution in Reverse: Engineering a D‐Xylose‐Specific Xylose Reductase (2008) (55)
- Synthetic biology: putting synthesis into biology (2010) (55)
- Recent advances in targeted genome engineering in mammalian systems (2012) (54)
- DNA punch cards for storing data on native DNA sequences via enzymatic nicking (2020) (53)
- Construction of plasmids with tunable copy numbers in Saccharomyces cerevisiae and their applications in pathway optimization and multiplex genome integration (2016) (53)
- A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis (2021) (53)
- Further improvement of phosphite dehydrogenase thermostability by saturation mutagenesis (2008) (53)
- Engineered Pentafunctional Minicellulosome for Simultaneous Saccharification and Ethanol Fermentation in Saccharomyces cerevisiae (2014) (53)
- Biosystems Design by Machine Learning. (2020) (52)
- Directed Evolution of Human Estrogen Receptor Variants with Significantly Enhanced Androgen Specificity and Affinity* (2004) (52)
- Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins (2013) (51)
- Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA (2014) (48)
- A New Class of Arylamine Oxygenases: Evidence that p‐Aminobenzoate N‐Oxygenase (AurF) is a Di‐iron Enzyme and Further Mechanistic Studies (2006) (48)
- Rapid creation of a novel protein function by in vitro coevolution. (2005) (48)
- Aryl-aldehyde formation in fungal polyketides: discovery and characterization of a distinct biosynthetic mechanism. (2014) (48)
- Directed evolution of xylose specific transporters to facilitate glucose‐xylose co‐utilization (2015) (47)
- In vivo biosensors: mechanisms, development, and applications (2018) (47)
- Rational pathway engineering of type I fatty acid synthase allows the biosynthesis of triacetic acid lactone from D-glucose in vivo. (2004) (46)
- Improving the thermostability of lipase Lip2 from Yarrowia lipolytica. (2013) (46)
- Directed evolution of soluble single-chain human class II MHC molecules. (2004) (46)
- Synthetic biology advances and applications in the biotechnology industry: a perspective (2018) (46)
- Metabolic engineering of Saccharomyces cerevisiae to produce 1-hexadecanol from xylose (2016) (46)
- Profiling of Microbial Colonies for High-Throughput Engineering of Multistep Enzymatic Reactions via Optically Guided Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (2017) (45)
- Engineered CRISPR/Cas9 system for multiplex genome engineering of polyploid industrial yeast strains. (2018) (45)
- A brief overview of synthetic biology research programs and roadmap studies in the United States (2016) (44)
- Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System (2018) (43)
- Directed evolution of a cellodextrin transporter for improved biofuel production under anaerobic conditions in Saccharomyces cerevisiae (2014) (42)
- Integrating biocatalysis with chemocatalysis for selective transformations. (2020) (42)
- Biosynthesis of 2-Hydroxyethylphosphonate, an Unexpected Intermediate Common to Multiple Phosphonate Biosynthetic Pathways* (2008) (41)
- TALE proteins search DNA using a rotationally decoupled mechanism. (2016) (41)
- Production of Adipic Acid from Sugar Beet Residue by Combined Biological and Chemical Catalysis (2015) (41)
- Recent advances in biosynthesis of fatty acids derived products in Saccharomyces cerevisiae via enhanced supply of precursor metabolites (2015) (40)
- A widespread pathway for substitution of adenine by diaminopurine in phage genomes (2021) (40)
- Directed evolution of novel protein functions. (2007) (40)
- TALEN outperforms Cas9 in editing heterochromatin target sites (2021) (39)
- Optimizing a biocatalyst for improved NAD(P)H regeneration: directed evolution of phosphite dehydrogenase. (2006) (39)
- Engineering of Enzymes for Selective Catalysis (2010) (39)
- FairyTALE: a high-throughput TAL effector synthesis platform. (2014) (39)
- CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci (2017) (38)
- Directed evolution of a highly efficient cellobiose utilizing pathway in an industrial Saccharomyces cerevisiae strain (2013) (38)
- In situ assembly of antifouling/bacterial silver nanoparticle-hydrogel composites with controlled particle release and matrix softening. (2015) (37)
- Discovery and engineering of a 1-butanol biosensor in Saccharomyces cerevisiae. (2017) (36)
- Directed Evolution of a Fluorinase for Improved Fluorination Efficiency with a Non-native Substrate. (2016) (36)
- Auroramycin: A Potent Antibiotic from Streptomyces roseosporus by CRISPR‐Cas9 Activation (2018) (36)
- Directed evolution of aniline dioxygenase for enhanced bioremediation of aromatic amines (2009) (35)
- A New Era of Genome Integration-Simply Cut and Paste! (2017) (35)
- Fully Automated One-Step Synthesis of Single-Transcript TALEN Pairs Using a Biological Foundry. (2017) (35)
- Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method (2017) (35)
- Mechanistic studies on the conversion of arylamines into arylnitro compounds by aminopyrrolnitrin oxygenase: identification of intermediates and kinetic studies. (2006) (35)
- A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data (2019) (34)
- CmlI is an N-oxygenase in the biosynthesis of chloramphenicol. (2012) (34)
- ECNet is an evolutionary context-integrated deep learning framework for protein engineering (2021) (34)
- Rapid prototyping of microbial cell factories via genome-scale engineering. (2015) (34)
- Directed evolution of homing endonuclease I-SceI with altered sequence specificity. (2009) (34)
- Genome-wide identification of natural RNA aptamers in prokaryotes and eukaryotes (2018) (33)
- Direct cloning of large genomic sequences (2012) (33)
- Orthogonal Genetic Regulation in Human Cells Using Chemically Induced CRISPR/Cas9 Activators. (2017) (33)
- Distinct structural elements dictate the specificity of the type III pentaketide synthase from Neurospora crassa. (2008) (33)
- Characterization and Engineering of the Adenylation Domain of a NRPS-Like Protein: A Potential Biocatalyst for Aldehyde Generation (2014) (33)
- DNA assembler: a synthetic biology tool for characterizing and engineering natural product gene clusters. (2012) (33)
- Coordinated induction of multi-gene pathways in Saccharomyces cerevisiae (2012) (32)
- Manual of Industrial Microbiology and Biotechnology, Third Edition (2010) (32)
- Genome-wide RNAi screen reveals the E3 SUMO-protein ligase gene SIZ1 as a novel determinant of furfural tolerance in Saccharomyces cerevisiae (2014) (32)
- Deciphering the late biosynthetic steps of antimalarial compound FR-900098. (2010) (32)
- Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides. (2019) (31)
- Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole (2018) (31)
- Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria (2019) (31)
- Cloning and characterization of a type III polyketide synthase from Aspergillus niger. (2011) (30)
- Construction and engineering of large biochemical pathways via DNA assembler. (2013) (30)
- Orthogonal Fatty Acid Biosynthetic Pathway Improves Fatty Acid Ethyl Ester Production in Saccharomyces cerevisiae. (2015) (30)
- Rapid identification of CD4+ T-cell epitopes using yeast displaying pathogen-derived peptide library. (2008) (29)
- Crystal structures of phosphite dehydrogenase provide insights into nicotinamide cofactor regeneration. (2012) (29)
- Investigating host dependence of xylose utilization in recombinant Saccharomyces cerevisiae strains using RNA-seq analysis (2013) (29)
- Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity. (2008) (29)
- A plug‐and‐play pathway refactoring workflow for natural product research in Escherichia coli and Saccharomyces cerevisiae (2017) (29)
- Metabolic engineering of threonine catabolism enables Saccharomyces cerevisiae to produce propionate under aerobic conditions (2022) (28)
- Synthetic Biology for Therapeutic Applications (2014) (28)
- Computational Tools for Discovering and Engineering Natural Product Biosynthetic Pathways (2019) (28)
- Identification and Characterization of the Flavin:NADH Reductase (PrnF) Involved in a Novel Two-Component Arylamine Oxygenase (2007) (28)
- Strategy for the Directed Evolution of a Peptide Ligase (1996) (28)
- Expanding the boundary of biocatalysis: design and optimization of in vitro tandem catalytic reactions for biochemical production (2018) (28)
- Role of Conserved Glycine in Zinc-dependent Medium Chain Dehydrogenase/Reductase Superfamily* (2012) (28)
- Manipulating Natural Product Biosynthetic Pathways via DNA Assembler (2014) (28)
- Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces (2020) (27)
- Mutagenic inverted repeat assisted genome engineering (MIRAGE) (2008) (27)
- Use of genome-editing tools to treat sickle cell disease (2016) (27)
- Emerging molecular biology tools and strategies for engineering natural product biosynthesis (2019) (27)
- Directed Evolution of Mesophilic Enzymes into Their Thermophilic Counterparts (1999) (27)
- Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging (2016) (25)
- Mechanistic investigation of a highly active phosphite dehydrogenase mutant and its application for NADPH regeneration (2005) (25)
- Directed evolution of phloroglucinol synthase PhlD with increased stability for phloroglucinol production (2013) (25)
- DNA assembler method for construction of zeaxanthin-producing strains of Saccharomyces cerevisiae. (2012) (25)
- Production of long chain alcohols and alkanes upon coexpression of an acyl-ACP reductase and aldehyde-deformylating oxygenase with a bacterial type-I fatty acid synthase in E. coli. (2015) (24)
- An efficient gene knock-in strategy using 5’-modified dsDNA donors with short homology arms (2019) (24)
- Directed Evolution Tools in Bioproduct and Bioprocess Development (2007) (24)
- Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis (2019) (24)
- Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination (2021) (23)
- A genetic toolbox for metabolic engineering of Issatchenkia orientalis. (2020) (23)
- Rapid Discovery of Glycocins through Pathway Refactoring in Escherichia coli. (2018) (23)
- Characterization of the N-oxygenase AurF from Streptomyces thioletus. (2014) (23)
- Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis. (2010) (23)
- Investigating glucose and xylose metabolism in Saccharomyces cerevisiae and Scheffersomyces stipitis via 13C metabolic flux analysis (2013) (22)
- Cloning and characterization of a galactitol 2-dehydrogenase from Rhizobium legumenosarum and its application in D-tagatose production. (2014) (21)
- A new biosensor for stilbenes and a cannabinoid enabled by genome mining of a transcriptional regulator. (2020) (21)
- Unraveling the iterative type I polyketide synthases hidden in Streptomyces (2020) (21)
- A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis. (2017) (21)
- Building biological foundries for next-generation synthetic biology (2015) (21)
- The Botrytis cinerea type III polyketide synthase shows unprecedented high catalytic efficiency toward long chain acyl-CoAs. (2012) (21)
- Recent advances in domesticating non‐model microorganisms (2020) (20)
- Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface. (2018) (20)
- Combinatorial pathway engineering for optimized production of the anti‐malarial FR900098 (2016) (20)
- Biomolecular Engineering (2005) (20)
- Rapid Screening of Lanthipeptide Analogs via In-Colony Removal of Leader Peptides in Escherichia coli. (2018) (20)
- Structure and engineering of L-arabinitol 4-dehydrogenase from Neurospora crassa. (2010) (20)
- Cloning, characterization, and mutational analysis of a highly active and stable l-arabinitol 4-dehydrogenase from Neurospora crassa (2007) (20)
- Accelerated genome engineering through multiplexing (2016) (20)
- Rapid and efficient galactose fermentation by engineered Saccharomyces cerevisiae. (2016) (20)
- Probing the Substrate Specificity of Aminopyrrolnitrin Oxygenase (PrnD) by Mutational Analysis (2006) (19)
- A mass spectrometry‐based high‐throughput screening method for engineering fatty acid synthases with improved production of medium‐chain fatty acids (2020) (19)
- Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis (2007) (19)
- SunnyTALEN: A second‐generation TALEN system for human genome editing (2014) (19)
- Crystal Structure and Computational Analyses Provide Insights into the Catalytic Mechanism of 2,4-Diacetylphloroglucinol Hydrolase PhlG from Pseudomonas fluorescens* (2009) (18)
- Two radical-dependent mechanisms for anaerobic degradation of the globally abundant organosulfur compound dihydroxypropanesulfonate (2020) (18)
- Directed Enzyme Evolution and High-Throughput Screening (2009) (18)
- Targeting Specificity of the CRISPR/Cas9 System. (2017) (18)
- Regeneration of Cofactors for Enzyme Biocatalysis (2004) (18)
- Engineering reprogrammable RNA-binding proteins for study and manipulation of the transcriptome. (2015) (18)
- Regulatory RNA-assisted genome engineering in microorganisms. (2015) (17)
- Cell surface display of functional human MHC class II proteins: yeast display versus insect cell display. (2011) (17)
- Directed evolution of orthogonal ligand specificity in a single scaffold. (2009) (17)
- A single-chain TALEN architecture for genome engineering. (2014) (17)
- Engineering oleaginous yeast Rhodotorula toruloides for overproduction of fatty acid ethyl esters (2021) (17)
- Identification of an important motif that controls the activity and specificity of sugar transporters (2016) (17)
- Unlocking nature's biosynthetic potential by directed genome evolution. (2020) (17)
- A new fluorescence complementation biosensor for detection of estrogenic compounds (2011) (16)
- A transaldolase-dependent sulfoglycolysis pathway in Bacillus megaterium DSM 1804. (2020) (16)
- Functional Reconstitution of a Pyruvate Dehydrogenase in the Cytosol of Saccharomyces cerevisiae through Lipoylation Machinery Engineering. (2016) (16)
- Discovery and Characterization of a Class IV Lanthipeptide with a Non-overlapping Ring Pattern. (2020) (16)
- Highly Efficient Single-Pot Scarless Golden Gate Assembly. (2019) (15)
- Characterization of Cas proteins for CRISPR‐Cas editing in streptomycetes (2019) (15)
- A highly efficient and sensitive screening method for trans-activation activity of estrogen receptors. (2003) (15)
- Challenges and opportunities in synthetic biology for chemical engineers. (2013) (14)
- High‐throughput Screening Methods Developed for Oxidoreductases (2006) (14)
- Outrunning Nature: Directed Evolution of Superior Biocatalysts (2004) (14)
- Flexible and Versatile Strategy for the Construction of Large Biochemical Pathways. (2016) (14)
- Staggered extension process in vitro DNA recombination. (2004) (13)
- RNAi assisted genome evolution unveils yeast mutants with improved xylose utilization (2018) (13)
- Investigation of the Role of Arg301 Identified in the X-ray Structure of Phosphite Dehydrogenase (2012) (13)
- Engineering Of Therapeutic Proteins (2009) (13)
- Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition (2006) (12)
- Investigation of the functional role of aldose 1-epimerase in engineered cellobiose utilization. (2013) (12)
- Family shuffling with single-stranded DNA. (2003) (12)
- Standardization for natural product synthetic biology. (2016) (11)
- Directed evolution of estrogen receptor proteins with altered ligand-binding specificities. (2009) (11)
- Genome-scale metabolic reconstruction of the non-model yeast Issatchenkia orientalis SD108 and its application to organic acids production (2020) (11)
- CTCF Promotes Long-range Enhancer-promoter Interactions and Lineage-specific Gene Expression in Mammalian Cells (2020) (11)
- Biochemical Characterization of an l-Xylulose Reductase from Neurospora crassa (2007) (11)
- Evolutionary Methods for Protein Engineering (2004) (11)
- A pH-indicator-based screen for hydrolytic haloalkane dehalogenase. (2003) (10)
- Structure-guided design and biosynthesis of a novel FR-900098 analogue as a potent Plasmodium falciparum 1-deoxy-D-xylulose-5-phosphate reductoisomerase (Dxr) inhibitor. (2015) (10)
- Mechanistically Diverse Pathways for Sulfoquinovose Degradation in Bacteria (2021) (10)
- Pathway Design, Engineering, and Optimization. (2016) (10)
- Chemical biotechnology: microbial solutions to global change. Editorial overview. (2008) (10)
- Recent advances in biocatalyst development in the pharmaceutical industry (2013) (10)
- Cloning, characterization, and engineering of fungal L-arabinitol dehydrogenases (2010) (10)
- Metabolic engineering of Rhodotorula toruloides IFO0880 improves C16 and C18 fatty alcohol production from synthetic media (2022) (9)
- Evolutionary context-integrated deep sequence modeling for protein engineering (2020) (9)
- Inducible Control of mRNA Transport Using Reprogrammable RNA-Binding Proteins (2017) (9)
- Creating new specific ligand-receptor pairs for transgene regulation. (2005) (9)
- Identification of novel metabolic engineering targets for S-adenosyl-L-Methionine production in Saccharomyces cerevisiae via genome-scale engineering. (2021) (9)
- New N-Acetyltransferase Fold in the Structure and Mechanism of the Phosphonate Biosynthetic Enzyme FrbF* (2011) (8)
- Customized optimization of metabolic pathways by combinatorial transcriptional engineering. (2013) (8)
- Cloning and characterization of a panel of mitochondrial targeting sequences for compartmentalization engineering in Saccharomyces cerevisiae (2021) (8)
- DNA Shuffling Method for Generating Estrogen Receptor α and β Chimeras in Yeast (2003) (8)
- DNA Punch Cards: Encoding Data on Native DNA Sequences via Nicking (2019) (8)
- An extended bacterial reductive pyrimidine degradation pathway that enables nitrogen release from β-alanine (2019) (8)
- Biocatalysis for Drug Discovery and Development (2016) (8)
- Industrial biotechnology: Tools and applications. (2015) (8)
- Drug Discovery and Development via Synthetic Biology (2013) (8)
- Mechanistic studies on the flavin:NADH reductase (PrnF) from Pseudomonas fluorescens involved in arylamine oxygenation. (2012) (8)
- Construction and screening of an antigen-derived peptide library displayed on yeast cell surface for CD4+ T cell epitope identification. (2013) (8)
- Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca. (2019) (7)
- Further biochemical studies on aminopyrrolnitrin oxygenase (PrnD). (2011) (7)
- PlasmidMaker is a versatile, automated, and high throughput end-to-end platform for plasmid construction (2022) (7)
- Visualizing Spatiotemporal Dynamics of Intercellular Mechanotransmission upon Wounding. (2018) (7)
- Characterization and application of the Fe(II) and α-ketoglutarate dependent hydroxylase FrbJ. (2011) (7)
- Orthogonal control of endogenous gene expression in mammalian cells using synthetic ligands (2013) (7)
- Delta Integration CRISPR-Cas (Di-CRISPR) in Saccharomyces cerevisiae. (2019) (6)
- A type III polyketide synthase from Rhizobium etli condenses malonyl CoAs to a heptaketide pyrone with unusually high catalytic efficiency. (2012) (6)
- Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense. (2019) (6)
- Synthetic biology : tools and applications (2013) (6)
- Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes (2019) (6)
- Development of Host-Orthogonal Genetic Systems for Synthetic Biology. (2021) (6)
- Divalent cations promote TALE DNA-binding specificity (2019) (6)
- A Pathway for Degradation of Uracil to Acetyl Coenzyme A in Bacillus megaterium (2020) (6)
- Biosynthetic engineering of the antifungal, anti-MRSA auroramycin (2019) (6)
- Expanding the Potential of Mammalian Genome Engineering via Targeted DNA Integration. (2021) (6)
- Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense (2019) (5)
- Precise Regulation of Cas9-Mediated Genome Engineering by Anti-CRISPR-Based Inducible CRISPR Controllers. (2021) (5)
- Combinatorial Design of a Highly Efficient Xylose Utilizing Pathway for Cellulosic (2012) (5)
- Strain Development by Whole-Cell Directed Evolution (2017) (5)
- Identification and Characterization of Citrus Peel Uronic Acid Oxidase (2020) (4)
- The Glycyl Radical Enzyme Arylacetate Decarboxylase from Olsenella scatoligenes (2021) (4)
- In vitro DNA recombination by random priming. (2003) (4)
- Publisher Correction: An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms (2020) (4)
- Characterization of the flavin monooxygenase involved in biosynthesis of the antimalarial FR-900098. (2019) (4)
- Author Correction: Building a global alliance of biofoundries (2019) (4)
- Improving protein functions by directed evolution (2009) (4)
- A scalable platform to discover antimicrobials of ribosomal origin (2022) (4)
- Industrial Applications of Enzymes as Catalysts (2010) (4)
- Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering (2011) (4)
- Targeting Speci fi city of the CRISPR / Cas 9 System (2017) (3)
- A gene cluster for taurine sulfur assimilation in an anaerobic human gut bacterium. (2019) (3)
- Mutagenic Inverted Repeats Assisted Genome Engineering (MIRAGE) in Saccharomyces cerevisiae: deletion of gal7. (2012) (3)
- A Pathway for Isethionate Dissimilation in Bacillus krulwichiae (2019) (3)
- Challenges and opportunities in C1-based biomanufacturing. (2022) (2)
- DNA shuffling method for generating estrogen receptor alpha and beta chimeras in yeast. (2003) (2)
- Proteome capacity constraints favor respiratory ATP generation (2022) (2)
- High‐Throughput Mass Spectrometry Complements Protein Engineering (2021) (2)
- Pathway engineering as an enabling synthetic biology tool (2013) (2)
- Two-Color Imaging of Non-repetitive Endogenous Loci in Human Cells. (2020) (2)
- Directed evolution of homing endonuclease with novel DNA sequence specificity (2005) (2)
- Directed Evolution: Novel and Improved Enzymes (2008) (2)
- Engineering Oleaginous Yeast Rhodosporidium Toruloides for Overproduction of Fatty Acid Ethyl Esters (2020) (2)
- Establishing carbon dioxide-based third-generation biorefinery for a sustainable low-carbon economy (2020) (2)
- RNAi-Assisted Genome Evolution (RAGE) in Saccharomyces cerevisiae. (2016) (2)
- Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion. (2018) (2)
- Directed Evolution of Replication-Competent Double-Stranded DNA Bacteriophage toward New Host Specificity. (2022) (2)
- Synthetic Biology – Metabolic Engineering (2018) (2)
- Applying Advanced DNA Assembly Methods to Generate Pathway Libraries (2018) (2)
- Nitrous Oxide Induced Subacute Combined Degeneration (2019) (2)
- Ferroic Materials for Smart Systems: From Fundamentals to Device Applications (2020) (2)
- Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system (2017) (1)
- Structural and Biochemical Investigation of UTP Cyclohydrolase (2021) (1)
- Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification. (2019) (1)
- Bioprocess for triacetic acid lactone (2004) (1)
- Biosynthetic engineering of the antifungal, anti-MRSA auroramycin (2020) (1)
- Engineered Biosynthesis of Natural Products in Heterologous Hosts (2015) (1)
- Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA (2014) (1)
- Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria (2019) (1)
- Drug Discovery and Development by Combinatorial Biosynthesis (2011) (1)
- Enzyme design by directed evolution (1998) (1)
- Profiling of d‐alanine production by the microbial isolates of rat gut microbiota (2021) (1)
- Engineering robust microorganisms for organic acid production (2021) (1)
- Issatchenkia orientalis SD 108 for succinic acid production (2016) (1)
- High‐throughput Screening Methods for Oxidoreductases (2008) (1)
- Building a global alliance of biofoundries (vol 10, 2040, 2019) (2019) (1)
- Editorial overview: Opportunities and challenges in synthetic biology. (2015) (1)
- A two-plasmid bacterial selection system for characterization and engineering of homing endonucleases. (2014) (1)
- Editorial: Synthetic biology and bioinformatics. (2016) (1)
- Comparative study of two Saccharomyces cerevisiae strains with kinetic models at genome-scale. (2023) (1)
- Can Deep Learning Solve the Cas9 Dilemma? (2021) (1)
- Combined and Iterative Use of Computational Design and Directed Evolution for Protein-Ligand Binding Design. (2016) (1)
- SynV and SynX: A story more than DNA synthesis (2017) (1)
- Building a global alliance of biofoundries (2019) (1)
- An Arylamine N-oxygenase involved in the biosynthesis of aromatic nitro compounds (2008) (0)
- Combinatorial optimization of a highly efficient xylose utilization pathway in saccharomyces cerevisiae (2011) (0)
- Pro fi ling of Microbial Colonies for High-Throughput Engineering of Multistep Enzymatic Reactions via Optically Guided Matrix-Assisted Laser Desorption / Ionization Mass Spectrometry (2017) (0)
- Genome-wide identification of natural RNA aptamers in prokaryotes and eukaryotes (2018) (0)
- Genome mining unveils a class of ribosomal peptides with two amino termini (2023) (0)
- Engineering chimeric RNA-binding proteins: Modular design, novel function (2014) (0)
- Directed evolution of phloroglucinol synthase PhlD with increased stability for phloroglucinol production (2013) (0)
- A diverse family of Type III polyketide synthases in Eucalyptus species. (2010) (0)
- Author ' s personal copy Biocatalyst development by directed evolution (2012) (0)
- Directed evolution of a phloroglucinol producing Type III polyketide synthase (2008) (0)
- Building biological foundries for next-generation synthetic biology (2015) (0)
- Microbial synthesis of phloroglucinol and xylitol (2008) (0)
- Author Correction: Building a global alliance of biofoundries (2019) (0)
- Systematic approaches to the protein engineering of highly specific receptor-ligand pairs (2005) (0)
- Programmable DNA-guided artificial restriction enzymes: Discovery, engineering, and applications (2017) (0)
- Minihemicellulosome Strains Displaying an Engineered Recombinant Saccharomyces cerevisiae Direct Conversion of Xylan to Ethanol (2012) (0)
- Direct observation of transcription activator-like effector (TALE) protein dynamics (2014) (0)
- Program Availability [5] Staggered Extension Process in Vitro Dna Recombination (2004) (0)
- Cellulose to Ethanol Saccharification and Fermentation of Minicellulosomes for Simultaneous Yeast Surface Display of Trifunctional (2014) (0)
- Directed evolution of homing endonuclease with altered DNA sequence specificity (2005) (0)
- Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole (2018) (0)
- [Illinois] Challenges and Opportunities in Synthetic Biology (2014) (0)
- Natural Product Discovery Top-Down Approaches Bottom-Up Approaches (2014) (0)
- Direct Observation of TALE Protein Search Dynamics along DNA (2015) (0)
- Editorial overview: Energy biotechnology. (2021) (0)
- 1 CRISPR-Cas 9 strategy for activation of silent Streptomyces biosynthetic gene clusters 1 (2017) (0)
- SPOT system protocol v2 (2021) (0)
- Phase-dependent Resonance Fluorescence Spectra For Two-level Atoms (1997) (0)
- Challenges and Opportunities in Synthetic Biology (2014) (0)
- Heterologous production of spectinabilin and analysis of the biosynthesis gene cluster (2008) (0)
- Pathway Engineering via Synthetic Biology (2012) (0)
- A ferredoxin-dependent dihydropyrimidine dehydrogenase in Clostridium chromiireducens (2020) (0)
- SPOT1 revised protocol v1 (2020) (0)
- Biochemical Investigation of 3‐Sulfopropionaldehyde Reductase HpfD (2021) (0)
- TriGORank: A Gene Ontology Enriched Learning-to-Rank Framework for Trigenic Fitness Prediction (2021) (0)
- Dow considers enzymatic dehalogenation (1999) (0)
- Tunnel engineering enables multifaceted improvements in halogenase (2022) (0)
- Expanding The Boundaries Of Biocatalysis (2016) (0)
- Directed evolution of artificial enzymes with new-to-nature functions. (2023) (0)
- Synthetic Biology : Challenges and Opportunities (2013) (0)
- Directed evolution of proteins for biomedical application (2005) (0)
- Discovery of Novel Natural Products via Synthetic Biology (2015) (0)
- Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB) (2015) (0)
- SPOT2 revised protocol v1 (2020) (0)
- Artificial Intelligence and Machine Learning for Bioenergy Research: Opportunities and Challenges (2023) (0)
- CrystalStructureandComputationalAnalysesProvideInsights intotheCatalyticMechanismof2,4-Diacetylphloroglucinol HydrolasePhlGfromPseudomonasfluorescens * (2010) (0)
- DNA punch cards for storing data on native DNA sequences via enzymatic nicking (2020) (0)
- Recombinaison de sequences de polynucleotides a l'aide de sequences d'amorces aleatoires ou definies (1998) (0)
- Engineering saccharomyces cerevisiae for biofuel production from lignocellulosic biomass (2011) (0)
- Enabling medium-chain fatty acid production in yeast via high-throughput MALDI MS- based enzyme engineering (2019) (0)
- A synthetic device for intracellular mRNA transport and local protein translation in eukaryotes (2014) (0)
- Phosphonate Biosynthetic Enzyme FrbF Structure and Mechanism of the-Acetyltransferase Fold in the N New Protein Structure and Folding : (2011) (0)
- Deciphering the late steps in FR900098 biosynthesis (2008) (0)
- Development of a phosphite dehydrogenase-based nicotinamide cofactor regeneration system (2005) (0)
- Re-writable DNA-based digital storage with random access (2016) (0)
- 3 Directed Enzyme Evolution and High-Throughput Screening (2009) (0)
- Protein engineering of gene switches and scissors for human gene therapy (2008) (0)
- Design and application of a kinetic model of lipid metabolism in Saccharomyces cerevisiae. (2022) (0)
- Xylose reductase hautement active provenant de neurospora crassa (2005) (0)
- CharacterizationoftheSubstrateSpecificityofPhlD,aTypeIII Polyketide Synthase fromPseudomonas fluorescens *□ (2006) (0)
- Engineering a fungal L-Arabinose pathway towards the utilization of pentose sugars for production of xylitol and ethanol (2008) (0)
- A step through the looking glass (2016) (0)
- Host‐Orthogonal Genetic Systems: Development of Host‐Orthogonal Genetic Systems for Synthetic Biology (Adv. Biology 3/2021) (2021) (0)
- Mining and application of constitutive promoters from Rhodosporidium toruloides (2023) (0)
- © 2010 Jing Du DISCOVERY AND CHARACTERIZATION OF PENTOSE-SPECIFIC TRANSPORTERS IN SACCHAROMYCES CEREVISIAE BY JING DU THESIS (2010) (0)
- A step through the looking glass (2016) (0)
- SynV and SynX: A story more than DNA synthesis (2017) (0)
- [Analytical method and comparison for static and dynamic headspace gas chromatography of anisole in water]. (2013) (0)
- in Saccharomyces cerevisiae Saccharification and Ethanol Fermentation Minicellulosome for Simultaneous Engineered Pentafunctional (2014) (0)
- Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution. (2020) (0)
- Anaerobic Hydroxyproline Degradation Involving C-N Cleavage by a Glycyl Radical Enzyme. (2022) (0)
- High-Throughput Screening or Selection Methods for Evolutionary Enzyme Engineering (2016) (0)
- Strain Improvement via Evolutionary Engineering (2012) (0)
- Advances in Succinic Acid (2019) (0)
- Lineage-specific control of promoter-enhancer interactions by CTCF during neural cell differentiation (2019) (0)
- Use of genome-editing tools to treat sickle cell disease (2016) (0)
- ' s personal copy Protein design for pathway engineering (2014) (0)
- para‐Aminobenzoate N‐Oxygenase (2009) (0)
- Engineered minicellulosomes for consolidated bioprocessing (2016) (0)
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